(Linear) (Six-base) MAPSORT of: KIEE4543.seq Check: 5339 from: 1 to: 1881 KIEE4543.orf With 182 enzymes: SgfI * March 22, 2012 18:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 903 911 1881 Size: 903 8 970 Fragments arranged by size: 970 903 8 AccI GT'mk_AC Cuts at: 0 1504 1881 Size: 1504 377 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 302 667 818 849 1726 1881 Size: 302 365 151 31 877 155 Fragments arranged by size: 877 365 302 155 151 31 AflIII A'CryG_T Cuts at: 0 962 1881 Size: 962 919 AvaI C'yCGr_G Cuts at: 0 1232 1881 Size: 1232 649 BanI G'GyrC_C Cuts at: 0 337 614 1881 Size: 337 277 1267 Fragments arranged by size: 1267 337 277 BanII G_rGCy'C Cuts at: 0 41 806 1167 1881 Size: 41 765 361 714 Fragments arranged by size: 765 714 361 41 BbsI GAAGACnn'nnnn_ Cuts at: 0 1714 1811 1881 Size: 1714 97 70 Fragments arranged by size: 1714 97 70 BbvCI CC'TCA_GC Cuts at: 0 603 638 811 1881 Size: 603 35 173 1070 Fragments arranged by size: 1070 603 173 35 BciVI GTATCCnnnnn_n' Cuts at: 0 73 1881 Size: 73 1808 BclI T'GATC_A Cuts at: 0 497 1881 Size: 497 1384 BfrBI ATG'CAT Cuts at: 0 1007 1881 Size: 1007 874 BglII A'GATC_T Cuts at: 0 1443 1881 Size: 1443 438 BmrI ACTGGGnnnn_n' Cuts at: 0 1167 1357 1578 1881 Size: 1167 190 221 303 Fragments arranged by size: 1167 303 221 190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 1881 Size: 793 1088 Bpu10I CC'TnA_GC Cuts at: 0 603 638 811 1881 Size: 603 35 173 1070 Fragments arranged by size: 1070 603 173 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 928 1390 1881 Size: 928 462 491 Fragments arranged by size: 928 491 462 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1772 1802 1881 Size: 1772 30 79 Fragments arranged by size: 1772 79 30 BseYI C'CCAG_C Cuts at: 0 1029 1881 Size: 1029 852 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1114 1881 Size: 1114 767 BsiHKAI G_wGCw'C Cuts at: 0 41 806 1881 Size: 41 765 1075 Fragments arranged by size: 1075 765 41 BsmI GAATG_Cn' Cuts at: 0 1009 1881 Size: 1009 872 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1171 1881 Size: 1171 710 Bsp1286I G_dGCh'C Cuts at: 0 41 806 1167 1881 Size: 41 765 361 714 Fragments arranged by size: 765 714 361 41 BspHI T'CATG_A Cuts at: 0 1835 1881 Size: 1835 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 903 911 1881 Size: 903 8 970 Fragments arranged by size: 970 903 8 BsrBI CCG'CTC Cuts at: 0 1191 1881 Size: 1191 690 BsrDI GCAATG_nn' Cuts at: 0 81 1881 Size: 81 1800 BsrFI r'CCGG_y Cuts at: 0 1037 1881 Size: 1037 844 BsrGI T'GTAC_A Cuts at: 0 1247 1881 Size: 1247 634 BstAPI GCAn_nnn'nTGC Cuts at: 0 1753 1881 Size: 1753 128 BstXI CCAn_nnnn'nTGG Cuts at: 0 1764 1881 Size: 1764 117 BstYI r'GATC_y Cuts at: 0 1443 1881 Size: 1443 438 BstZ17I GTA'TAC Cuts at: 0 1505 1881 Size: 1505 376 BtgI C'CryG_G Cuts at: 0 234 1881 Size: 234 1647 ClaI AT'CG_AT Cuts at: 0 1667 1881 Size: 1667 214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1044 1079 1881 Size: 1044 35 802 Fragments arranged by size: 1044 802 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1561 1881 Size: 1561 320 EaeI y'GGCC_r Cuts at: 0 2 1536 1881 Size: 2 1534 345 Fragments arranged by size: 1534 345 2 EarI CTCTTCn'nnn_ Cuts at: 0 794 1181 1867 1881 Size: 794 387 686 14 Fragments arranged by size: 794 686 387 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1036 1881 Size: 1036 845 EcoICRI GAG'CTC Cuts at: 0 39 804 1881 Size: 39 765 1077 Fragments arranged by size: 1077 765 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 302 667 793 818 849 1726 1881 Size: 302 365 126 25 31 877 155 Fragments arranged by size: 877 365 302 155 126 31 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 649 1881 Size: 649 1232 EcoO109I rG'GnC_Cy Cuts at: 0 642 1626 1881 Size: 642 984 255 Fragments arranged by size: 984 642 255 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 390 1104 1881 Size: 390 714 777 Fragments arranged by size: 777 714 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1430 1462 1701 1733 1881 Size: 1430 32 239 32 148 Fragments arranged by size: 1430 239 148 32 32 HincII GTy'rAC Cuts at: 0 381 627 1227 1311 1566 1881 Size: 381 246 600 84 255 315 Fragments arranged by size: 600 381 315 255 246 84 HindIII A'AGCT_T Cuts at: 0 47 890 1366 1881 Size: 47 843 476 515 Fragments arranged by size: 843 515 476 47 HpaI GTT'AAC Cuts at: 0 1227 1881 Size: 1227 654 MfeI C'AATT_G Cuts at: 0 521 1881 Size: 521 1360 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 450 1701 1881 Size: 450 1251 180 Fragments arranged by size: 1251 450 180 MscI TGG'CCA Cuts at: 0 4 1538 1881 Size: 4 1534 343 Fragments arranged by size: 1534 343 4 MslI CAynn'nnrTG Cuts at: 0 433 1762 1840 1881 Size: 433 1329 78 41 Fragments arranged by size: 1329 433 78 41 NaeI GCC'GGC Cuts at: 0 1039 1881 Size: 1039 842 NcoI C'CATG_G Cuts at: 0 234 1881 Size: 234 1647 NgoMIV G'CCGG_C Cuts at: 0 1037 1881 Size: 1037 844 NsiI A_TGCA'T Cuts at: 0 1009 1881 Size: 1009 872 NspI r_CATG'y Cuts at: 0 427 966 1011 1881 Size: 427 539 45 870 Fragments arranged by size: 870 539 427 45 PasI CC'CwG_GG Cuts at: 0 1218 1881 Size: 1218 663 PciI A'CATG_T Cuts at: 0 962 1881 Size: 962 919 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 586 618 1881 Size: 586 32 1263 Fragments arranged by size: 1263 586 32 PpuMI rG'GwC_Cy Cuts at: 0 642 1626 1881 Size: 642 984 255 Fragments arranged by size: 984 642 255 PspXI vC'TCGA_Gb Cuts at: 0 1232 1881 Size: 1232 649 PstI C_TGCA'G Cuts at: 0 1016 1881 Size: 1016 865 SacI G_AGCT'C Cuts at: 0 41 806 1881 Size: 41 765 1075 Fragments arranged by size: 1075 765 41 SapI GCTCTTCn'nnn_ Cuts at: 0 794 1181 1881 Size: 794 387 700 Fragments arranged by size: 794 700 387 ScaI AGT'ACT Cuts at: 0 870 1881 Size: 870 1011 SexAI A'CCwGG_T Cuts at: 0 1680 1808 1881 Size: 1680 128 73 Fragments arranged by size: 1680 128 73 SfcI C'TryA_G Cuts at: 0 176 1012 1881 Size: 176 836 869 Fragments arranged by size: 869 836 176 SmlI C'TyrA_G Cuts at: 0 907 1232 1369 1881 Size: 907 325 137 512 Fragments arranged by size: 907 512 325 137 SphI G_CATG'C Cuts at: 0 1011 1881 Size: 1011 870 StyI C'CwwG_G Cuts at: 0 234 1881 Size: 234 1647 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 355 1210 1560 1881 Size: 355 855 350 321 Fragments arranged by size: 855 355 350 321 TatI w'GTAC_w Cuts at: 0 868 1247 1639 1881 Size: 868 379 392 242 Fragments arranged by size: 868 392 379 242 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1499 1531 1772 1804 1881 Size: 1499 32 241 32 77 Fragments arranged by size: 1499 241 77 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1086 1881 Size: 1086 795 XhoI C'TCGA_G Cuts at: 0 1232 1881 Size: 1232 649 XmnI GAAnn'nnTTC Cuts at: 0 941 1881 Size: 941 940 Enzymes that do cut: AarI AccI AcuI AflIII AvaI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BstZ17I BtgI ClaI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI NaeI NcoI NgoMIV NsiI NspI PasI PciI PpiI PpuMI PspXI PstI SacI SapI ScaI SexAI SfcI SmlI SphI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BglI BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BspEI BssHII BssSI BstBI BstEII Bsu36I BtgZI BtsI DraI DraIII EagI EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI MluI MspA1I NarI NdeI NheI NotI NruI PacI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI