################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspI BalI BamHI BbeI BbrPI BbvCI BglI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CpoI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FseI HaeII HgaI Hin1I HpaI KasI KspI MluI MluNI MroI MscI MspA1I MvnI NarI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 798 GGTACC G^GTACC AflII 301 CTTAAG C^TTAAG Asp700I 2413 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 798 GGTACC G^GTACC AvaI 344 CTCGAG C^[CT]CG[GA]G AviII 456 TGCGCA TGC^GCA AvrII 1429 CCTAGG C^CTAGG BanI 798 GGTACC G^G[CT][GA]CC BbsI 1320 GAAGAC GAAGAC(2/6) BcgI 312 GCAACTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 301 CTTAAG C^TTAAG BglII 268 AGATCT A^GATCT BlnI 1429 CCTAGG C^CTAGG BlpI 2295 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2295 GCTGAGC GC^T[ACGT]AGC BpuAI 1320 GAAGAC GAAGAC(2/6) BsaAI 1667 CACGTA [CT]AC^GT[GA] BseRI 1969 GAGGAG GAGGAG(10/8) BsoBI 344 CTCGAG C^[CT]CG[GA]G BspDI 1012 ATCGAT AT^CGAT BsrDI 1886 CATTGC GCAATG(2/0) BsrFI 2307 GCCGGC [GA]^CCGG[CT] BssSI 433 CTCGTG CACGAG(-5/-1) BstXI 2028 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2295 GCTGAGC GC^T[ACGT]AGC CfoI 457 GCGC GCG^C Cfr10I 2307 GCCGGC [GA]^CCGG[CT] ClaI 1012 ATCGAT AT^CGAT DraII 2274 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 686 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 383 CCATGG C^C[GA][CT]GG EaeI 2511 TGGCCG [CT]^GGCC[GA] EcoO109I 2274 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 2041 GAATTC G^AATTC EcoT22I 2063 ATGCAT ATGCA^T FauI 481 GCGGG CCCGC(4/6) FspI 456 TGCGCA TGC^GCA HhaI 457 GCGC GCG^C HinP1I 457 GCGC G^CGC HincII 1651 GTTGAC GT[CT]^[GA]AC HindII 1651 GTTGAC GT[CT]^[GA]AC KpnI 798 GGTACC GGTAC^C MaeII 1668 ACGT A^CGT MfeI 1165 CAATTG C^AATTG MunI 1165 CAATTG C^AATTG NaeI 2307 GCCGGC GCC^GGC NcoI 383 CCATGG C^CATGG NgoMIV 2307 GCCGGC G^CCGGC NsiI 2063 ATGCAT ATGCA^T PaeR7I 344 CTCGAG C^TCGAG PflMI 1566 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2274 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1133 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2377 GAGCTC GAGCT^C SapI 964 GCTCTTC GCTCTTC(1/4) SexAI 1385 ACCTGGT A^CC[AT]GGT SphI 712 GCATGC GCATG^C Van91I 1566 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 271 TCTAGA T^CTAGA XcmI 561 CCAGTTTGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 344 CTCGAG C^TCGAG XmnI 2413 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 481 GCGG CCGC(-3/-1) 2479 GCGG AflIII 650 ACATGT A^C[GA][CT]GT 1078 ACATGT AhdI 673 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1981 GACGATGAGTC AlwNI 1028 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 1567 CAGCCTCTG AspEI 673 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1981 GACGATGAGTC AspHI 2082 GTGCTC G[AT]GC[AT]^C 2377 GAGCTC BanII 1866 GAGCCC G[GA]GC[CT]^C 2377 GAGCTC BciVI 5 GGATAC GTATCC(6/5) 87 GGATAC BclI 585 TGATCA T^GATCA 1710 TGATCA BpmI 2471 CTCCAG CTGGAG(16/14) 1835 CTGGAG BsaBI 786 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 910 GATGTTGATC BsaI 1628 GAGACC GGTCTC(1/5) 413 GGTCTC BsiHKAI 2082 GTGCTC G[AT]GC[AT]^C 2377 GAGCTC BspLU11I 650 ACATGT A^CATGT 1078 ACATGT BstYI 268 AGATCT [GA]^GATC[CT] 516 GGATCT DraI 549 TTTAAA TTT^AAA 2398 TTTAAA Eam1105I 673 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1981 GACGATGAGTC FbaI 585 TGATCA T^GATCA 1710 TGATCA MamI 786 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 910 GATGTTGATC MflI 268 AGATCT [GA]^GATC[CT] 516 GGATCT PstI 2050 CTGCAG CTGCA^G 2237 CTGCAG ScaI 1183 AGTACT AGT^ACT 1210 AGTACT SfcI 2050 CTGCAG C^T[GA][CT]AG 2237 CTGCAG SspI 2122 AATATT AAT^ATT 2529 AATATT TaqI 345 TCGA T^CGA 1013 TCGA TthHB8I 345 TCGA T^CGA 1013 TCGA XhoII 268 AGATCT [GA]^GATC[CT] 516 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BbvI BcnI BfaI BmyI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI