################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BlnI BpmI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Cfr10I CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 157 GTAGAC GT^[AC][GT]AC AcyI 637 GACGCC G[GA]^CG[CT]C AlwNI 823 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 160 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 363 GGACC G^G[AT]CC BbvCI 66 CCTCAGC CCTCAGC(-5/-2) BglII 785 AGATCT A^GATCT BlpI 378 GCTCAGC GC^T[ACGT]AGC Bpu1102I 378 GCTCAGC GC^T[ACGT]AGC BsaHI 637 GACGCC G[GA]^CG[CT]C BsgI 1048 GTGCAG GTGCAG(16/14) BsmBI 353 GAGACG CGTCTC(1/5) BspDI 562 ATCGAT AT^CGAT BspHI 206 TCATGA T^CATGA BspMI 844 ACCTGC ACCTGC(4/8) BstEII 193 GGTCACC G^GT[ACGT]ACC BstPI 193 GGTCACC G^GT[ACGT]ACC CelII 378 GCTCAGC GC^T[ACGT]AGC ClaI 562 ATCGAT AT^CGAT EcoO65I 193 GGTCACC G^GT[ACGT]ACC HaeII 449 AGCGCC [GA]GCGC^[CT] Hin1I 637 GACGCC G[GA]^CG[CT]C MseI 791 TTAA T^TAA NcoI 716 CCATGG C^CATGG NspI 885 GCATGT [GA]CATG^[CT] PflFI 160 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 822 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 206 TCATGA T^CATGA SapI 528 GAAGAGC GCTCTTC(1/4) ScaI 515 AGTACT AGT^ACT SmlI 343 CTCAAG C^T[CT][GA]AG Tru9I 791 TTAA T^TAA Tsp45I 194 GTCAC ^GT[GC]AC Tsp509I 37 AATT ^AATT Tth111I 160 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 822 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccII 388 CGCG CG^CG 625 CGCG AflIII 419 ACACGT A^C[GA][CT]GT 1004 ACGTGT AocI 46 CCTGAGG CC^T[ACGT]AGG 611 CCTCAGG AspHI 376 GTGCTC G[AT]GC[AT]^C 838 GTGCTC BalI 89 TGGCCA TGG^CCA 801 TGGCCA BanII 990 GGGCTC G[GA]GC[CT]^C 1026 GGGCTC BcnI 453 CCGGG CC^[GC]GG 705 CCGGG BglI 287 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 491 GCCTGACGGGC BsaAI 373 TACGTG [CT]AC^GT[GA] 835 TACGTG BsiHKAI 376 GTGCTC G[AT]GC[AT]^C 838 GTGCTC BsmAI 136 GAGAC GTCTC(1/5) 353 GAGAC BsmFI 128 GGGAC GGGAC(10/14) 594 GTCCC BstUI 388 CGCG CG^CG 625 CGCG BstYI 785 AGATCT [GA]^GATC[CT] 929 AGATCC Bsu36I 46 CCTGAGG CC^T[ACGT]AGG 611 CCTCAGG CfoI 450 GCGC GCG^C 898 GCGC EarI 528 GAAGAG CTCTTC(1/4) 954 GAAGAG Eco81I 46 CCTGAGG CC^T[ACGT]AGG 611 CCTCAGG EcoT14I 92 CCAAGG C^C[AT][AT]GG 716 CCATGG HapII 453 CCGG C^CGG 705 CCGG HhaI 450 GCGC GCG^C 898 GCGC HinP1I 450 GCGC G^CGC 898 GCGC HinfI 138 GACTC G^A[ACGT]TC 238 GAGTC HpaII 453 CCGG C^CGG 705 CCGG Ksp632I 528 GAAGAG CTCTTC(1/4) 954 GAAGAG MaeIII 11 GTAAC ^GT[ACGT]AC 194 GTCAC MflI 785 AGATCT [GA]^GATC[CT] 929 AGATCC MluNI 89 TGGCCA TGG^CCA 801 TGGCCA MscI 89 TGGCCA TGG^CCA 801 TGGCCA MslI 207 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 711 CATCTCCATG MspI 453 CCGG C^CGG 705 CCGG MvnI 388 CGCG CG^CG 625 CGCG NciI 453 CCGGG CC^[GC]GG 705 CCGGG NlaIV 363 GGACCC GG[ACGT]^[ACGT]CC 818 GGAACC PleI 138 GACTC GAGTC(4/5) 238 GAGTC PstI 769 CTGCAG CTGCA^G 949 CTGCAG PvuII 980 CAGCTG CAG^CTG 1051 CAGCTG SfaNI 407 GCATC GCATC(5/9) 647 GCATC StyI 92 CCAAGG C^C[AT][AT]GG 716 CCATGG XhoII 785 AGATCT [GA]^GATC[CT] 929 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HgaI HphI ItaI MaeII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfcI TaqI TseI TspRI TthHB8I