(Circular) (Six-base) MAPSORT of: pFN21AE4364.seq Check: 8881 from: 1 to: 5848 pFN21AE4364 5848 bp With 182 enzymes: SgfI * March 28, 2012 13:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2858 2858 Size: 5848 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2163 3191 1937 Size: 226 1028 4594 Fragments arranged by size: 4594 1028 226 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 3911 5295 5563 1632 Size: 920 1359 1384 268 1917 Fragments arranged by size: 1917 1384 1359 920 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 2424 3009 4747 5724 1203 Size: 1221 585 1738 977 1327 Fragments arranged by size: 1738 1327 1221 977 585 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2891 1184 Size: 1707 4141 AlwNI CAG_nnn'CTG Cuts at: 2833 3591 5163 2833 Size: 758 1572 3518 Fragments arranged by size: 3518 1572 758 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 3157 3383 3535 5644 1493 Size: 1664 226 152 2109 1697 Fragments arranged by size: 2109 1697 1664 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3169 4624 618 Size: 325 217 627 1382 1455 1842 Fragments arranged by size: 1842 1455 1382 627 325 217 BanII G_rGCy'C Cuts at: 1391 2999 3035 3167 1391 Size: 1608 36 132 4072 Fragments arranged by size: 4072 1608 132 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BbvCI CC'TCA_GC Cuts at: 2072 2072 Size: 5848 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4039 4073 1903 Size: 34 50 34 2018 34 3678 Fragments arranged by size: 3678 2018 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2298 2502 4459 Size: 107 122 71 631 605 626 204 1957 Cuts at: 4459 136 Size: 1525 Fragments arranged by size: 1957 1525 631 626 605 204 122 107 71 BglII A'GATC_T Cuts at: 2790 5843 2790 Size: 3053 2795 BlpI GC'TnA_GC Cuts at: 2384 3265 2384 Size: 881 4967 Bme1580I G_kGCm'C Cuts at: 1790 2870 3909 5065 1790 Size: 1080 1039 1156 2573 Fragments arranged by size: 2573 1156 1080 1039 BmgBI CAC'GTC Cuts at: 1318 3010 1318 Size: 1692 4156 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2880 3579 4537 Size: 416 53 83 399 24 1463 699 958 Cuts at: 4537 442 Size: 1753 Fragments arranged by size: 1753 1463 958 699 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2491 2491 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4508 1239 Size: 3269 2579 Bpu10I CC'TnA_GC Cuts at: 2072 2909 2072 Size: 837 5011 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3973 4838 5136 2333 Size: 1640 865 298 3045 Fragments arranged by size: 3045 1640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2380 2842 3639 493 Size: 711 1176 462 797 2702 Fragments arranged by size: 2702 1176 797 711 462 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2643 3655 4037 4672 Size: 53 83 186 936 1110 1012 382 635 Cuts at: 4672 275 Size: 1451 Fragments arranged by size: 1451 1110 1012 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3086 3116 1178 Size: 30 165 30 1683 30 3910 Fragments arranged by size: 3910 1683 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2072 2255 2711 2801 2945 1186 Size: 883 3 183 456 90 144 4089 Fragments arranged by size: 4089 883 456 183 144 90 3 BseYI C'CCAG_C Cuts at: 3028 5051 5547 3028 Size: 2023 496 3329 Fragments arranged by size: 3329 2023 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3074 1163 Size: 573 1338 3937 Fragments arranged by size: 3937 1338 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 2847 3909 3994 5065 2385 Size: 462 1062 85 1071 3168 Fragments arranged by size: 3168 1071 1062 462 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 BsmBI CGTCTCn'nnnn_ Cuts at: 2352 2352 Size: 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2847 2870 2999 3035 3167 3909 Size: 399 595 462 23 129 36 132 742 Cuts at: 3909 3994 5065 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 462 399 132 129 85 36 23 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 2211 3737 5467 2211 Size: 1526 1730 2592 Fragments arranged by size: 2592 1730 1526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2858 3203 844 Size: 2014 345 3489 Fragments arranged by size: 3489 2014 345 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4492 4679 1700 Size: 110 2682 187 2869 Fragments arranged by size: 2869 2682 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 2198 1184 Size: 1014 4834 BstYI r'GATC_y Cuts at: 1972 2790 2934 3178 3545 3930 3947 5399 5485 Size: 818 144 244 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 818 385 367 346 244 144 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2052 2617 2052 Size: 565 5283 BtgI C'CryG_G Cuts at: 513 1065 2217 2293 2446 2647 2721 2991 513 Size: 552 1152 76 153 201 74 270 3370 Fragments arranged by size: 3370 1152 552 270 201 153 76 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 2437 3364 4158 4178 2437 Size: 927 794 20 4107 Fragments arranged by size: 4107 927 794 20 ClaI AT'CG_AT Cuts at: 1554 2568 1554 Size: 1014 4834 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2404 2806 Size: 54 1425 50 275 18 264 310 402 Cuts at: 2806 3295 4184 5813 8 Size: 489 889 1629 43 Fragments arranged by size: 1629 1425 889 489 402 310 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2528 2954 3778 1353 Size: 1175 426 824 3423 Fragments arranged by size: 3423 1175 824 426 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2744 4435 4809 4955 387 Size: 1064 138 1155 1691 374 146 1280 Fragments arranged by size: 1691 1280 1155 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2552 3911 4508 5295 5563 1239 Size: 393 920 1359 597 787 268 1524 Fragments arranged by size: 1524 1359 920 787 597 393 268 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2284 2284 Size: 5848 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2458 4995 1678 Size: 780 2537 2531 Fragments arranged by size: 2537 2531 780 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2228 2260 4489 Size: 32 135 32 560 32 64 32 2229 Cuts at: 4489 4521 4563 4595 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2229 560 135 64 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 3039 3084 4962 5146 1305 Size: 1578 156 45 1878 184 2007 Fragments arranged by size: 2007 1878 1578 184 156 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2808 10 Size: 54 1425 343 264 712 3050 Fragments arranged by size: 3050 1425 712 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2720 3806 4165 4324 518 Size: 648 1050 504 1086 359 159 2042 Fragments arranged by size: 2042 1086 1050 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 2605 2987 3058 3264 3941 5089 5334 1606 Size: 999 382 71 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 999 677 382 245 206 71 NcoI C'CATG_G Cuts at: 513 1065 2721 513 Size: 552 1656 3640 Fragments arranged by size: 3640 1656 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2894 3206 4751 1132 Size: 97 1665 312 1545 2229 Fragments arranged by size: 2229 1665 1545 312 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 2833 1108 Size: 1725 4123 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PstI C_TGCA'G Cuts at: 830 2778 2958 3200 830 Size: 1948 180 242 3478 Fragments arranged by size: 3478 1948 242 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2987 3058 2987 Size: 71 5777 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SapI GCTCTTCn'nnn_ Cuts at: 2528 2528 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 2522 4096 5808 2522 Size: 1574 1712 2562 Fragments arranged by size: 2562 1712 1574 SfcI C'TryA_G Cuts at: 826 1045 2160 2774 2954 3196 4331 5012 5203 Size: 219 1115 614 180 242 1135 681 191 Cuts at: 5203 826 Size: 1471 Fragments arranged by size: 1471 1135 1115 681 614 242 219 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 3952 4853 5115 820 Size: 197 926 405 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 405 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2097 2721 3287 513 Size: 552 1032 624 566 3074 Fragments arranged by size: 3074 1032 624 566 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 2677 3875 3892 4045 4230 4675 Size: 1255 456 641 1198 17 153 185 445 Cuts at: 4675 325 Size: 1498 Fragments arranged by size: 1498 1255 1198 641 456 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 4094 5806 Size: 274 80 33 51 1443 543 1574 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1712 1574 1443 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5808 5840 5808 Size: 32 5816 Tth111I GACn'n_nGTC Cuts at: 1316 2168 1316 Size: 852 4996 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI