Restriction map for
pFN21AB6968
(Circular) (Six-base) MAPSORT of: pFN21AB6968.seq Check: 8888 from: 1 to: 5428
pFN21AB6968 5428 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3491 4875 5143 1632
Size: 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 2627 3171 4743 2627
Size: 544 1572 3312
Fragments arranged by size:
3312 1572 544
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2008 2737 2963 3115 5224 1493
Size: 515 729 226 152 2109 1697
Fragments arranged by size:
2109 1697 729 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 1870
Size: 73 810 4545
Fragments arranged by size:
4545 810 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2749 4204 618
Size: 325 217 627 962 1455 1842
Fragments arranged by size:
1842 1455 962 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2747 1391
Size: 1356 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2263 3320 4530 2263
Size: 1057 1210 3161
Fragments arranged by size:
3161 1210 1057
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4645 1790
Size: 1699 1156 2573
Fragments arranged by size:
2573 1699 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2295 2370 3159
Size: 416 53 83 399 24 878 75 789
Cuts at: 3159 4117 442
Size: 958 1753
Fragments arranged by size:
1753 958 878 789 416 399 83 75
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4088 1239
Size: 2849 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2399 3553 4418 4716 2399
Size: 1154 865 298 3111
Fragments arranged by size:
3111 1154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 4091 882
Size: 617 519 2073 2219
Fragments arranged by size:
2219 2073 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3235 3617 4252 275
Size: 53 83 186 936 1702 382 635 1451
Fragments arranged by size:
1702 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 1178
Size: 30 165 30 784 30 4389
Fragments arranged by size:
4389 784 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5428
BseYI C'CCAG_C
Cuts at: 4631 5127 4631
Size: 496 4932
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4855
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2878 3639 4667 1540
Size: 275 186 877 761 1028 2301
Fragments arranged by size:
2301 1028 877 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3489 3574 4645 3489
Size: 85 1071 4272
Fragments arranged by size:
4272 1071 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2747 3489 3574 4645 1391
Size: 399 957 742 85 1071 2174
Fragments arranged by size:
2174 1071 957 742 399 85
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2408 2783 844
Size: 1564 375 3489
Fragments arranged by size:
3489 1564 375
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4072 4259 1700
Size: 110 2262 187 2869
Fragments arranged by size:
2869 2262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 3488 4500 3488
Size: 1012 4416
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2758 3125 3510 3527 4979 5065 5077 5423
Size: 786 367 385 17 1452 86 12 346
Cuts at: 5423 1972
Size: 1977
Fragments arranged by size:
1977 1452 786 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2028 513
Size: 552 963 3913
Fragments arranged by size:
3913 963 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2392 2427 529
Size: 35 1828 35 3530
Fragments arranged by size:
3530 1828 35 35
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2221 2875 3764
Size: 54 1425 50 275 18 391 654 889
Cuts at: 3764 5393 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 654 391 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2322 2484 2496 3358 1353
Size: 969 162 12 862 3423
Fragments arranged by size:
3423 969 862 162 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2350 4015 4389 4535 387
Size: 1064 138 761 1665 374 146 1280
Fragments arranged by size:
1665 1280 1064 761 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3491 4088 4875 5143 1239
Size: 393 1859 597 787 268 1524
Fragments arranged by size:
1859 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 5428
EcoRI G'AATT_C
Cuts at: 2008 2737 3115 2008
Size: 729 378 4321
Fragments arranged by size:
4321 729 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2565 2565
Size: 5428
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 4575 1678
Size: 2897 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 2495 2527 2585
Size: 32 135 32 587 32 304 32 58
Cuts at: 2585 2617 4069 4101 4143 4175 1373
Size: 32 1452 32 42 32 2626
Fragments arranged by size:
2626 1452 587 304 135 58 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 2213 748
Size: 1465 3963
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2099 2173 4542 4726 1305
Size: 794 74 2369 184 2007
Fragments arranged by size:
2369 2007 794 184 74
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3386 3745 3904 518
Size: 648 2220 359 159 2042
Fragments arranged by size:
2220 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2844 3521 4669 4914 1606
Size: 1238 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2028 513
Size: 552 963 3913
Fragments arranged by size:
3913 963 552
NdeI CA'TA_TG
Cuts at: 387 2148 387
Size: 1761 3667
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2786 4331 1132
Size: 97 1557 1545 2229
Fragments arranged by size:
2229 1557 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PfoI T'CCnGG_A
Cuts at: 2349 2349
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2780 830
Size: 1950 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SexAI A'CCwGG_T
Cuts at: 2452 2452
Size: 5428
SfcI C'TryA_G
Cuts at: 826 1045 2776 3911 4592 4783 826
Size: 219 1731 1135 681 191 1471
Fragments arranged by size:
1731 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 3532 4433 4695 820
Size: 197 926 435 1154 901 262 1553
Fragments arranged by size:
1553 1154 926 901 435 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 2020 1517
Size: 503 4925
StyI C'CwwG_G
Cuts at: 513 1065 2028 2062 2867 513
Size: 552 963 34 805 3074
Fragments arranged by size:
3074 963 805 552 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 3455 3472 3625 3810 4255 325
Size: 1255 617 1258 17 153 185 445 1498
Fragments arranged by size:
1498 1258 1255 617 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2187 2219 5388 5420 2187
Size: 32 3169 32 2195
Fragments arranged by size:
3169 2195 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2552 2764 2552
Size: 212 5216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2357 2357
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI