################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanII BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MaeI MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 105 GAATTT [GA]^AATT[CT] AlwNI 299 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 105 GAATTT [GA]^AATT[CT] BalI 362 TGGCCA TGG^CCA BamHI 356 GGATCC G^GATCC BanI 345 GGCACC G^G[CT][GA]CC BbrPI 424 CACGTG CAC^GTG BmyI 81 GTGCCC G[AGT]GC[ACT]^C BpmI 467 CTCCAG CTGGAG(16/14) BsaAI 424 CACGTG [CT]AC^GT[GA] BsaWI 93 TCCGGT [AT]^CCGG[AT] BsgI 97 GTGCAG GTGCAG(16/14) BsmFI 57 GGGAC GGGAC(10/14) Bsp1286I 81 GTGCCC G[AGT]GC[ACT]^C BspMI 403 ACCTGC ACCTGC(4/8) BssSI 388 CTCGTG CACGAG(-5/-1) EaeI 362 TGGCCA [CT]^GGCC[GA] EarI 40 CTCTTC CTCTTC(1/4) HapII 94 CCGG C^CGG HgaI 176 GACGC GACGC(5/10) HincII 289 GTTAAC GT[CT]^[GA]AC HindII 289 GTTAAC GT[CT]^[GA]AC HpaI 289 GTTAAC GTT^AAC HpaII 94 CCGG C^CGG Ksp632I 40 CTCTTC CTCTTC(1/4) MaeIII 535 GTCAC ^GT[ACGT]AC MluNI 362 TGGCCA TGG^CCA MscI 362 TGGCCA TGG^CCA MspI 94 CCGG C^CGG PleI 481 GACTC GAGTC(4/5) PmaCI 424 CACGTG CAC^GTG PmlI 424 CACGTG CAC^GTG SexAI 542 ACCAGGT A^CC[AT]GGT TaqI 269 TCGA T^CGA TfiI 261 GAATC G^A[AT]TC Tsp45I 535 GTCAC ^GT[GC]AC TthHB8I 269 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AciI 26 CCGC CCGC(-3/-1) 49 GCGG BstF5I 183 CATCC GGATG(2/0) 411 CATCC Cac8I 34 GCTGGC GC[ACGT]^[ACGT]GC 303 GCCTGC FokI 183 CATCC GGATG(9/13) 411 CATCC HinfI 261 GAATC G^A[ACGT]TC 481 GACTC MaeII 113 ACGT A^CGT 425 ACGT NlaIV 345 GGCACC GG[ACGT]^[ACGT]CC 356 GGATCC Tsp509I 106 AATT ^AATT 144 AATT TspRI 29 CACTG CA[GC]TG[ACGT][ACGT]^ 476 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AfaI AluI AlwI BbvI BsrI BssKI BstNI BstYI DpnII EcoRII Fnu4HI HaeIII HphI ItaI MboI MboII MflI MnlI MseI MvaI MwoI NdeII RsaI Sau3AI ScrFI SfaNI Tru9I TseI XhoII