(Circular) (Six-base) MAPSORT of: pFN21AB6318.seq Check: 1634 from: 1 to: 5548 pFN21AB6318 5548 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2468 3611 4995 5263 1632 Size: 651 185 1143 1384 268 1917 Fragments arranged by size: 1917 1384 1143 651 268 185 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AlwNI CAG_nnn'CTG Cuts at: 2741 3291 4863 2741 Size: 550 1572 3426 Fragments arranged by size: 3426 1572 550 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2442 2518 2857 3083 3235 5344 1493 Size: 949 76 339 226 152 2109 1697 Fragments arranged by size: 2109 1697 949 339 226 152 76 AseI AT'TA_AT Cuts at: 160 1032 2274 2585 2835 4102 160 Size: 872 1242 311 250 1267 1606 Fragments arranged by size: 1606 1267 1242 872 311 250 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2100 2183 2873 1870 Size: 73 157 83 690 4545 Fragments arranged by size: 4545 690 157 83 73 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2869 4324 618 Size: 325 217 627 1082 1455 1842 Fragments arranged by size: 1842 1455 1082 627 325 217 BanII G_rGCy'C Cuts at: 1391 2058 2217 2867 1391 Size: 667 159 650 4072 Fragments arranged by size: 4072 667 650 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 928 Size: 971 213 4364 Fragments arranged by size: 4364 971 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2400 2823 3440 4650 2400 Size: 423 617 1210 3298 Fragments arranged by size: 3298 1210 617 423 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4159 136 Size: 107 122 71 631 605 2487 1525 Fragments arranged by size: 2487 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5543 5543 Size: 5548 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 3609 4765 1790 Size: 1819 1156 2573 Fragments arranged by size: 2573 1819 1156 BmgBI CAC'GTC Cuts at: 1318 2764 1318 Size: 1446 4102 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 4208 1239 Size: 1388 1581 2579 Fragments arranged by size: 2579 1581 1388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3673 4538 4836 3673 Size: 865 298 4385 Fragments arranged by size: 4385 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2092 4211 882 Size: 617 593 2119 2219 Fragments arranged by size: 2219 2119 617 593 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3355 3737 4372 275 Size: 53 83 186 936 1822 382 635 1451 Fragments arranged by size: 1822 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 1186 Size: 1030 4518 BseYI C'CCAG_C Cuts at: 4751 5247 4751 Size: 496 5052 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2107 1163 Size: 573 371 4604 Fragments arranged by size: 4604 573 371 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2167 2998 3759 4787 1540 Size: 275 186 166 831 761 1028 2301 Fragments arranged by size: 2301 1028 831 761 275 186 166 BsiHKAI G_wGCw'C Cuts at: 2058 3609 3694 4765 2058 Size: 1551 85 1071 2841 Fragments arranged by size: 2841 1551 1071 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2217 2867 3609 3694 4765 1391 Size: 399 268 159 650 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 650 399 268 159 85 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 844 Size: 2059 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2219 3435 1394 Size: 429 396 1216 3507 Fragments arranged by size: 3507 1216 429 396 BsrDI GCAATG_nn' Cuts at: 65 1248 2816 4043 4217 65 Size: 1183 1568 1227 174 1396 Fragments arranged by size: 1568 1396 1227 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4192 4379 1700 Size: 110 2382 187 2869 Fragments arranged by size: 2869 2382 187 110 BsrGI T'GTAC_A Cuts at: 96 2467 96 Size: 2371 3177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2400 2878 3245 3630 3647 5099 5185 5197 Size: 428 478 367 385 17 1452 86 12 Cuts at: 5197 5543 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 478 428 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2042 513 Size: 552 939 38 4019 Fragments arranged by size: 4019 939 552 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 2240 3064 3858 3878 2240 Size: 824 794 20 3910 Fragments arranged by size: 3910 824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2164 2822 2995 Size: 54 1425 50 275 18 334 658 173 Cuts at: 2995 3884 5513 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 658 334 275 173 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2164 2995 1537 Size: 275 352 831 4090 Fragments arranged by size: 4090 831 352 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3478 1353 Size: 2125 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2080 4135 4509 4655 387 Size: 1064 138 491 2055 374 146 1280 Fragments arranged by size: 2055 1280 1064 491 374 146 138 EcoICRI GAG'CTC Cuts at: 2056 2056 Size: 5548 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2468 2627 3611 4208 4995 5263 Size: 393 651 185 159 984 597 787 268 Cuts at: 5263 1239 Size: 1524 Fragments arranged by size: 1524 984 787 651 597 393 268 185 159 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FspI TGC'GCA Cuts at: 2232 4054 2232 Size: 1822 3726 HaeII r_GCGC'y Cuts at: 1678 4695 1678 Size: 3017 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373 Size: 32 135 32 2617 32 42 32 2626 Fragments arranged by size: 2626 2617 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 2295 748 Size: 1547 4001 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3506 3865 4024 518 Size: 648 2340 359 159 2042 Fragments arranged by size: 2340 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2252 2964 3641 4789 5034 1606 Size: 646 712 677 1148 245 2120 Fragments arranged by size: 2120 1148 712 677 646 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2042 513 Size: 552 939 38 4019 Fragments arranged by size: 4019 939 552 38 NdeI CA'TA_TG Cuts at: 387 2511 387 Size: 2124 3424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2164 2995 2164 Size: 831 4717 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5548 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 1108 Size: 975 4573 PfoI T'CCnGG_A Cuts at: 2403 2747 2403 Size: 344 5204 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 2711 3118 2711 Size: 407 5141 PstI C_TGCA'G Cuts at: 830 2900 830 Size: 2070 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2252 2252 Size: 5548 SacI G_AGCT'C Cuts at: 2058 2058 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 2334 3796 5508 2334 Size: 1462 1712 2374 Fragments arranged by size: 2374 1712 1462 SfcI C'TryA_G Cuts at: 826 1045 2896 4031 4712 4903 826 Size: 219 1851 1135 681 191 1471 Fragments arranged by size: 1851 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2875 1872 Size: 1003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3652 4553 4815 820 Size: 197 926 1709 901 262 1553 Fragments arranged by size: 1709 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 2081 1517 Size: 564 4984 StyI C'CwwG_G Cuts at: 513 1065 2004 2042 2082 2987 513 Size: 552 939 38 40 905 3074 Fragments arranged by size: 3074 939 905 552 40 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 3575 3592 3745 3930 4375 325 Size: 1255 579 1416 17 153 185 445 1498 Fragments arranged by size: 1498 1416 1255 579 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2332 2467 3794 Size: 274 80 33 51 1443 355 135 1327 Cuts at: 3794 5506 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1327 355 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2401 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2873 1870 Size: 1003 4545 XmnI GAAnn'nnTTC Cuts at: 2109 3677 2109 Size: 1568 3980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI