(Circular) (Six-base) MAPSORT of: pFN21AE1485.seq Check: 1215 from: 1 to: 5974 pFN21AE1485 5974 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2235 3062 3317 1937 Size: 298 827 255 4594 Fragments arranged by size: 4594 827 298 255 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4037 5421 5689 1632 Size: 2405 1384 268 1917 Fragments arranged by size: 2405 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 2796 1166 Size: 1630 4344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 4858 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 2768 Size: 32 5942 AlwNI CAG_nnn'CTG Cuts at: 3717 5289 3717 Size: 1572 4402 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2268 2405 3283 3509 3661 5770 1493 Size: 775 137 878 226 152 2109 1697 Fragments arranged by size: 2109 1697 878 775 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2343 3299 1870 Size: 73 400 956 4545 Fragments arranged by size: 4545 956 400 73 BamHI G'GATC_C Cuts at: 2453 3304 3671 2453 Size: 851 367 4756 Fragments arranged by size: 4756 851 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3293 1391 Size: 1902 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2056 928 Size: 971 157 4846 Fragments arranged by size: 4846 971 157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BfrBI ATG'CAT Cuts at: 2225 2932 2225 Size: 707 5267 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2302 5969 2302 Size: 3667 2307 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 3176 4035 5191 1790 Size: 1386 859 1156 2573 Fragments arranged by size: 2573 1386 1156 859 BmgBI CAC'GTC Cuts at: 1318 2296 1318 Size: 978 4996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3123 4634 1239 Size: 1884 1511 2579 Fragments arranged by size: 2579 1884 1511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2911 4099 4964 5262 2911 Size: 1188 865 298 3623 Fragments arranged by size: 3623 1188 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5974 BseYI C'CCAG_C Cuts at: 2943 5177 5673 2943 Size: 2234 496 3244 Fragments arranged by size: 3244 2234 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4035 4120 5191 4035 Size: 85 1071 4818 Fragments arranged by size: 4818 1071 85 BsmI GAATG_Cn' Cuts at: 2932 3485 3578 2932 Size: 553 93 5328 Fragments arranged by size: 5328 553 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3176 3293 4035 4120 5191 1391 Size: 399 1386 117 742 85 1071 2174 Fragments arranged by size: 2174 1386 1071 742 399 117 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2265 3861 1394 Size: 429 442 1596 3507 Fragments arranged by size: 3507 1596 442 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2749 4469 4643 65 Size: 1183 1501 1720 174 1396 Fragments arranged by size: 1720 1501 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2170 2665 4618 4805 1700 Size: 110 360 495 1953 187 2869 Fragments arranged by size: 2869 1953 495 360 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 3029 794 Size: 2235 3739 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2302 2453 2559 3120 3304 3671 4056 4073 Size: 330 151 106 561 184 367 385 17 Cuts at: 4073 5525 5611 5623 5969 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 561 385 367 346 330 184 151 106 86 17 12 BstZ17I GTA'TAC Cuts at: 3063 3063 Size: 5974 Bsu36I CC'TnA_GG Cuts at: 2639 2639 Size: 5974 BtgI C'CryG_G Cuts at: 513 1065 3073 513 Size: 552 2008 3414 Fragments arranged by size: 3414 2008 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2284 2883 3490 4284 4304 2284 Size: 599 607 794 20 3954 Fragments arranged by size: 3954 794 607 599 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 2256 2487 3278 2256 Size: 231 791 4952 Fragments arranged by size: 4952 791 231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 3070 3421 Size: 54 1425 50 275 18 250 990 351 Cuts at: 3421 4310 5939 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 990 889 351 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2103 3904 1353 Size: 698 52 1801 3423 Fragments arranged by size: 3423 1801 698 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3123 4037 4634 5421 5689 1239 Size: 393 1491 914 597 787 268 1524 Fragments arranged by size: 1524 1491 914 787 597 393 268 EcoRI G'AATT_C Cuts at: 2268 3283 3661 2268 Size: 1015 378 4581 Fragments arranged by size: 4581 1015 378 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2544 2576 2987 3019 4615 Size: 32 135 32 972 32 411 32 1596 Cuts at: 4615 4647 4689 4721 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1596 972 411 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 3318 3564 669 Size: 988 281 298 1082 246 3079 Fragments arranged by size: 3079 1082 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 2009 3225 748 Size: 1261 1216 3497 Fragments arranged by size: 3497 1261 1216 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3153 3573 3153 Size: 420 5554 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2349 5088 5272 1305 Size: 1044 2739 184 2007 Fragments arranged by size: 2739 2007 1044 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2082 3072 10 Size: 54 1425 343 250 990 2912 Fragments arranged by size: 2912 1425 990 343 250 54 MslI CAynn'nnrTG Cuts at: 518 1166 2256 2796 2927 3072 3932 4291 4450 Size: 648 1090 540 131 145 860 359 159 Cuts at: 4450 518 Size: 2042 Fragments arranged by size: 2042 1090 860 648 540 359 159 145 131 MspA1I CmG'CkG Cuts at: 1606 2180 3236 3390 4067 5215 5460 1606 Size: 574 1056 154 677 1148 245 2120 Fragments arranged by size: 2120 1148 1056 677 574 245 154 NcoI C'CATG_G Cuts at: 513 1065 3073 513 Size: 552 2008 3414 Fragments arranged by size: 3414 2008 552 NdeI CA'TA_TG Cuts at: 387 2149 2525 387 Size: 1762 376 3836 Fragments arranged by size: 3836 1762 376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NsiI A_TGCA'T Cuts at: 2227 2934 2227 Size: 707 5267 NspI r_CATG'y Cuts at: 1132 1229 2063 2932 3260 3332 4877 1132 Size: 97 834 869 328 72 1545 2229 Fragments arranged by size: 2229 1545 869 834 328 97 72 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2556 2793 1108 Size: 1448 237 4289 Fragments arranged by size: 4289 1448 237 PfoI T'CCnGG_A Cuts at: 3100 3100 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 2411 3544 2411 Size: 1133 4841 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2025 2057 2025 Size: 32 5942 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2180 2180 Size: 5974 SalI G'TCGA_C Cuts at: 1936 2234 3316 1936 Size: 298 1082 4594 Fragments arranged by size: 4594 1082 298 SapI GCTCTTCn'nnn_ Cuts at: 2103 2103 Size: 5974 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SexAI A'CCwGG_T Cuts at: 3056 3056 Size: 5974 SfcI C'TryA_G Cuts at: 826 1045 2129 3217 3322 4457 5138 5329 826 Size: 219 1084 1088 105 1135 681 191 1471 Fragments arranged by size: 1471 1135 1088 1084 681 219 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2890 4078 4979 5241 820 Size: 197 926 947 1188 901 262 1553 Fragments arranged by size: 1553 1188 947 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 3044 152 Size: 2892 3082 SphI G_CATG'C Cuts at: 1132 2932 3332 1132 Size: 1800 400 3774 Fragments arranged by size: 3774 1800 400 SspI AAT'ATT Cuts at: 5 52 3143 3898 5 Size: 47 3091 755 2081 Fragments arranged by size: 3091 2081 755 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 3073 3413 513 Size: 552 2008 340 3074 Fragments arranged by size: 3074 2008 552 340 SwaI ATTT'AAAT Cuts at: 2256 2256 Size: 5974 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4001 4018 4171 4356 4801 325 Size: 1255 2421 17 153 185 445 1498 Fragments arranged by size: 2421 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 2521 2553 5934 5966 2438 Size: 32 51 32 3381 32 2446 Fragments arranged by size: 3381 2446 51 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2333 1316 Size: 1017 4957 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 3024 4103 3024 Size: 1079 4895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI XcmI