Restriction map for pFN21AE1485
 (Linear) (Six-base) MAPSORT of: KIEE1485.seq  Check: 1503  from: 1  to: 1269



KIEE1485.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AccI GT'mk_AC

Cuts at:      0     230    1057    1269 
   Size:        230     827     212

  Fragments arranged by size: 

                 827     230     212
 
AleI CACnn'nnGTG

Cuts at:      0     791    1269 
   Size:        791     478
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     295    1269 
   Size:        295     974
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     763     795    1269 
   Size:        763      32     474

  Fragments arranged by size: 

                 763     474      32
 
ApoI r'AATT_y

Cuts at:      0     263     400    1269 
   Size:        263     137     869

  Fragments arranged by size: 

                 869     263     137
 
AvaI C'yCGr_G

Cuts at:      0     338    1269 
   Size:        338     931
 
BamHI G'GATC_C

Cuts at:      0     448    1269 
   Size:        448     821
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      51    1269 
   Size:         51    1218
 
BfrBI ATG'CAT

Cuts at:      0     220     927    1269 
   Size:        220     707     342

  Fragments arranged by size: 

                 707     342     220
 
BglII A'GATC_T

Cuts at:      0     297    1269 
   Size:        297     972
 
Bme1580I G_kGCm'C

Cuts at:      0    1171    1269 
   Size:       1171      98
 
BmgBI CAC'GTC

Cuts at:      0     291    1269 
   Size:        291     978
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1118    1269 
   Size:       1118     151
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     906    1269 
   Size:        906     363
 
BseYI C'CCAG_C

Cuts at:      0     938    1269 
   Size:        938     331
 
BsmI GAATG_Cn'

Cuts at:      0     927    1269 
   Size:        927     342
 
Bsp1286I G_dGCh'C

Cuts at:      0    1171    1269 
   Size:       1171      98
 
BsrBI CCG'CTC

Cuts at:      0     260    1269 
   Size:        260    1009
 
BsrDI GCAATG_nn'

Cuts at:      0     744    1269 
   Size:        744     525
 
BsrFI r'CCGG_y

Cuts at:      0     165     660    1269 
   Size:        165     495     609

  Fragments arranged by size: 

                 609     495     165
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1024    1269 
   Size:       1024     245
 
BstYI r'GATC_y

Cuts at:      0     297     448     554    1115    1269 
   Size:        297     151     106     561     154

  Fragments arranged by size: 

                 561     297     154     151     106
 
BstZ17I GTA'TAC

Cuts at:      0    1058    1269 
   Size:       1058     211
 
Bsu36I CC'TnA_GG

Cuts at:      0     634    1269 
   Size:        634     635
 
BtgI C'CryG_G

Cuts at:      0    1068    1269 
   Size:       1068     201
 
BtsI GCAGTG_nn'

Cuts at:      0     279     878    1269 
   Size:        279     599     391

  Fragments arranged by size: 

                 599     391     279
 
DraI TTT'AAA

Cuts at:      0     251     482    1269 
   Size:        251     231     787

  Fragments arranged by size: 

                 787     251     231
 
EaeI y'GGCC_r

Cuts at:      0      75    1065    1269 
   Size:         75     990     204

  Fragments arranged by size: 

                 990     204      75
 
EarI CTCTTCn'nnn_

Cuts at:      0      46      98    1269 
   Size:         46      52    1171

  Fragments arranged by size: 

                1171      52      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1118    1269 
   Size:       1118     151
 
EcoRI G'AATT_C

Cuts at:      0     263    1269 
   Size:        263    1006
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     539     571     982    1014    1269 
   Size:        539      32     411      32     255

  Fragments arranged by size: 

                 539     411     255      32      32
 
HincII GTy'rAC

Cuts at:      0     231    1269 
   Size:        231    1038
 
HindIII A'AGCT_T

Cuts at:      0       4    1220    1269 
   Size:          4    1216      49

  Fragments arranged by size: 

                1216      49       4
 
MfeI C'AATT_G

Cuts at:      0    1148    1269 
   Size:       1148     121
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     344    1269 
   Size:        344     925
 
MscI TGG'CCA

Cuts at:      0      77    1067    1269 
   Size:         77     990     202

  Fragments arranged by size: 

                 990     202      77
 
MslI CAynn'nnrTG

Cuts at:      0     251     791     922    1067    1269 
   Size:        251     540     131     145     202

  Fragments arranged by size: 

                 540     251     202     145     131
 
MspA1I CmG'CkG

Cuts at:      0     175    1231    1269 
   Size:        175    1056      38

  Fragments arranged by size: 

                1056     175      38
 
NcoI C'CATG_G

Cuts at:      0    1068    1269 
   Size:       1068     201
 
NdeI CA'TA_TG

Cuts at:      0     144     520    1269 
   Size:        144     376     749

  Fragments arranged by size: 

                 749     376     144
 
NsiI A_TGCA'T

Cuts at:      0     222     929    1269 
   Size:        222     707     340

  Fragments arranged by size: 

                 707     340     222
 
NspI r_CATG'y

Cuts at:      0      58     927    1255    1269 
   Size:         58     869     328      14

  Fragments arranged by size: 

                 869     328      58      14
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     551     788    1269 
   Size:        551     237     481

  Fragments arranged by size: 

                 551     481     237
 
PfoI T'CCnGG_A

Cuts at:      0    1095    1269 
   Size:       1095     174
 
PsiI TTA'TAA

Cuts at:      0     406    1269 
   Size:        406     863
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      20      52    1269 
   Size:         20      32    1217

  Fragments arranged by size: 

                1217      32      20
 
PvuII CAG'CTG

Cuts at:      0     175    1269 
   Size:        175    1094
 
SalI G'TCGA_C

Cuts at:      0     229    1269 
   Size:        229    1040
 
SapI GCTCTTCn'nnn_

Cuts at:      0      98    1269 
   Size:         98    1171
 
SexAI A'CCwGG_T

Cuts at:      0    1051    1269 
   Size:       1051     218
 
SfcI C'TryA_G

Cuts at:      0     124    1212    1269 
   Size:        124    1088      57

  Fragments arranged by size: 

                1088     124      57
 
SmlI C'TyrA_G

Cuts at:      0     885    1269 
   Size:        885     384
 
SpeI A'CTAG_T

Cuts at:      0    1039    1269 
   Size:       1039     230
 
SphI G_CATG'C

Cuts at:      0     927    1269 
   Size:        927     342
 
SspI AAT'ATT

Cuts at:      0    1138    1269 
   Size:       1138     131
 
StyI C'CwwG_G

Cuts at:      0    1068    1269 
   Size:       1068     201
 
SwaI ATTT'AAAT

Cuts at:      0     251    1269 
   Size:        251    1018
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     433     465     516     548    1269 
   Size:        433      32      51      32     721

  Fragments arranged by size: 

                 721     433      51      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     328    1269 
   Size:        328     941
 
XmnI GAAnn'nnTTC

Cuts at:      0    1019    1269 
   Size:       1019     250
 

 Enzymes that do cut:

     AccI     AleI     AlfI     AloI     ApoI     AvaI    BamHI     BbsI
    BfrBI    BglII Bme1580I    BmgBI     BpmI    BpuEI    BseYI     BsmI
 Bsp1286I    BsrBI    BsrDI    BsrFI   BstAPI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI    EcoRI    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NsiI     NspI    PflMI     PfoI     PsiI     PsrI    PvuII
     SalI     SapI    SexAI     SfcI     SmlI     SpeI     SphI     SspI
     StyI     SwaI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI     BlpI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PstI     PvuI    RsrII     SacI    SacII    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     StuI
    TaqII     TatI     XbaI     XcmI     XhoI     XmaI     ZraI
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