(Circular) (Six-base) MAPSORT of: pFN21AB7770.seq Check: 8091 from: 1 to: 6136 pFN21AB7770 6136 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3355 3355 Size: 6136 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3287 4199 5583 5851 1632 Size: 1655 912 1384 268 1917 Fragments arranged by size: 1917 1655 1384 912 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 3349 5035 6012 1203 Size: 2146 1686 977 1327 Fragments arranged by size: 2146 1686 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 3338 4865 3338 Size: 1527 4609 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AlwNI CAG_nnn'CTG Cuts at: 3361 3879 5451 3361 Size: 518 1572 4046 Fragments arranged by size: 4046 1572 518 ApaI G_GGCC'C Cuts at: 2644 2644 Size: 6136 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 2761 3445 3671 3823 5932 1493 Size: 1268 684 226 152 2109 1697 Fragments arranged by size: 2109 1697 1268 684 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 3461 1870 Size: 73 1518 4545 Fragments arranged by size: 4545 1518 73 BamHI G'GATC_C Cuts at: 2463 3466 3833 2463 Size: 1003 367 4766 Fragments arranged by size: 4766 1003 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3457 4912 618 Size: 325 217 627 1670 1455 1842 Fragments arranged by size: 1842 1670 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2644 3102 3261 3455 1391 Size: 1253 458 159 194 4072 Fragments arranged by size: 4072 1253 458 194 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2163 2282 3231 3383 3389 928 Size: 971 189 75 119 949 152 6 3675 Fragments arranged by size: 3675 971 949 189 152 119 75 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4327 4361 1903 Size: 34 2390 34 3678 Fragments arranged by size: 3678 2390 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 3369 1548 Size: 1821 4315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2035 4747 136 Size: 107 122 71 631 605 363 2712 1525 Fragments arranged by size: 2712 1525 631 605 363 122 107 71 BglII A'GATC_T Cuts at: 2592 3417 6131 2592 Size: 825 2714 2597 Fragments arranged by size: 2714 2597 825 BlpI GC'TnA_GC Cuts at: 3553 3553 Size: 6136 Bme1580I G_kGCm'C Cuts at: 1790 2644 4197 5353 1790 Size: 854 1553 1156 2573 Fragments arranged by size: 2573 1553 1156 854 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3867 4825 442 Size: 416 53 83 399 24 2450 958 1753 Fragments arranged by size: 2450 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2116 2581 3245 3275 3296 4796 1239 Size: 877 465 664 30 21 1500 2579 Fragments arranged by size: 2579 1500 877 664 465 30 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4261 5126 5424 4261 Size: 865 298 4973 Fragments arranged by size: 4973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3183 4799 882 Size: 617 1684 1616 2219 Fragments arranged by size: 2219 1684 1616 617 BsaAI yAC'GTr Cuts at: 493 1204 3352 3927 493 Size: 711 2148 575 2702 Fragments arranged by size: 2702 2148 711 575 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3943 4325 4960 275 Size: 53 83 186 936 2410 382 635 1451 Fragments arranged by size: 2410 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2409 4569 4967 5241 5388 5799 2409 Size: 2160 398 274 147 411 2746 Fragments arranged by size: 2746 2160 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 2567 2597 3089 Size: 30 165 30 675 30 459 30 492 Cuts at: 3089 3119 1178 Size: 30 4195 Fragments arranged by size: 4195 675 492 459 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2945 1186 Size: 1759 4377 BseYI C'CCAG_C Cuts at: 5339 5835 5339 Size: 496 5640 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2903 3374 1163 Size: 573 1167 471 3925 Fragments arranged by size: 3925 1167 573 471 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4347 5375 1540 Size: 275 186 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3261 4197 4282 5353 3261 Size: 936 85 1071 4044 Fragments arranged by size: 4044 1071 936 85 BsmI GAATG_Cn' Cuts at: 2910 3647 3740 2910 Size: 737 93 5306 Fragments arranged by size: 5306 737 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2644 3102 3261 3455 4197 4282 5353 Size: 399 854 458 159 194 742 85 1071 Cuts at: 5353 1391 Size: 2174 Fragments arranged by size: 2174 1071 854 742 458 399 194 159 85 BspEI T'CCGG_A Cuts at: 2409 5799 2409 Size: 3390 2746 BspHI T'CATG_A Cuts at: 2299 3045 4025 5755 2299 Size: 746 980 1730 2680 Fragments arranged by size: 2680 1730 980 746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3249 3355 3491 844 Size: 2405 106 136 3489 Fragments arranged by size: 3489 2405 136 106 BsrBI CCG'CTC Cuts at: 1394 1823 3406 4023 1394 Size: 429 1583 617 3507 Fragments arranged by size: 3507 1583 617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4780 4967 1700 Size: 110 2970 187 2869 Fragments arranged by size: 2970 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 2972 4196 5208 2972 Size: 1224 1012 3900 Fragments arranged by size: 3900 1224 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3361 794 Size: 2567 3569 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6136 BstYI r'GATC_y Cuts at: 1972 2065 2179 2463 2592 3417 3466 3833 4218 Size: 93 114 284 129 825 49 367 385 Cuts at: 4218 4235 5687 5773 5785 6131 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 825 385 367 346 284 129 114 93 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2010 2061 2871 513 Size: 552 945 51 810 3778 Fragments arranged by size: 3778 945 810 552 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 2526 3652 4446 4466 2526 Size: 1126 794 20 4196 Fragments arranged by size: 4196 1126 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2029 2064 2223 2258 529 Size: 35 1465 35 159 35 4407 Fragments arranged by size: 4407 1465 159 35 35 35 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2835 3583 4472 Size: 54 1425 50 275 18 1005 748 889 Cuts at: 4472 6101 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1005 889 748 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2298 4066 1353 Size: 945 1768 3423 Fragments arranged by size: 3423 1768 945 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2569 2603 4723 5097 5243 387 Size: 1064 138 980 34 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 980 374 146 138 34 EcoICRI GAG'CTC Cuts at: 3259 3259 Size: 6136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2116 2581 3245 3275 3287 3296 4199 Size: 393 484 465 664 30 12 9 903 Cuts at: 4199 4796 5583 5851 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 903 787 664 597 484 465 393 268 30 12 9 EcoO109I rG'GnC_Cy Cuts at: 2641 2641 Size: 6136 EcoRI G'AATT_C Cuts at: 2761 3445 3823 2761 Size: 684 378 5074 Fragments arranged by size: 5074 684 378 EcoRV GAT'ATC Cuts at: 2104 2605 2104 Size: 501 5635 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 6136 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 5283 1678 Size: 3605 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2269 3324 3356 4777 Size: 32 135 32 665 32 1055 32 1421 Cuts at: 4777 4809 4851 4883 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1421 1055 665 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2017 2125 3480 3726 669 Size: 988 281 79 108 1355 246 3079 Fragments arranged by size: 3079 1355 988 281 246 108 79 HindIII A'AGCT_T Cuts at: 748 2355 2429 748 Size: 1607 74 4455 Fragments arranged by size: 4455 1607 74 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 2328 3735 2328 Size: 1407 4729 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 2441 5250 5434 1305 Size: 1032 104 2809 184 2007 Fragments arranged by size: 2809 2007 1032 184 104 MscI TGG'CCA Cuts at: 10 64 1489 1832 2837 10 Size: 54 1425 343 1005 3309 Fragments arranged by size: 3309 1425 1005 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2024 2385 2658 4094 4453 4612 Size: 648 843 15 361 273 1436 359 159 Cuts at: 4612 518 Size: 2042 Fragments arranged by size: 2042 1436 843 648 361 359 273 159 15 MspA1I CmG'CkG Cuts at: 1606 2614 2630 2686 3265 3295 3552 4229 5377 Size: 1008 16 56 579 30 257 677 1148 Cuts at: 5377 5622 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1008 677 579 257 245 56 30 16 NcoI C'CATG_G Cuts at: 513 1065 2010 513 Size: 552 945 4639 Fragments arranged by size: 4639 945 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3494 5039 1132 Size: 97 2265 1545 2229 Fragments arranged by size: 2265 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6136 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PmlI CAC'GTG Cuts at: 3352 3352 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3393 1613 Size: 1780 4356 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PspOMI G'GGCC_C Cuts at: 2640 2640 Size: 6136 PstI C_TGCA'G Cuts at: 830 2817 3366 3414 3488 830 Size: 1987 549 48 74 3478 Fragments arranged by size: 3478 1987 549 74 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2614 3265 3295 2614 Size: 651 30 5455 Fragments arranged by size: 5455 651 30 SacI G_AGCT'C Cuts at: 3261 3261 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SapI GCTCTTCn'nnn_ Cuts at: 2298 2298 Size: 6136 SbfI CC_TGCA'GG Cuts at: 2817 3414 3488 2817 Size: 597 74 5465 Fragments arranged by size: 5465 597 74 ScaI AGT'ACT Cuts at: 2495 4384 6096 2495 Size: 1889 1712 2535 Fragments arranged by size: 2535 1889 1712 SfcI C'TryA_G Cuts at: 826 1045 2813 3362 3410 3484 4619 5300 5491 Size: 219 1768 549 48 74 1135 681 191 Cuts at: 5491 826 Size: 1471 Fragments arranged by size: 1768 1471 1135 681 549 219 191 74 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4240 5141 5403 820 Size: 197 926 2297 901 262 1553 Fragments arranged by size: 2297 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 3162 4060 5 Size: 47 3110 898 2081 Fragments arranged by size: 3110 2081 898 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2010 3037 3575 513 Size: 552 945 1027 538 3074 Fragments arranged by size: 3074 1027 945 552 538 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4163 4180 4333 4518 4963 325 Size: 1255 2583 17 153 185 445 1498 Fragments arranged by size: 2583 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2493 4382 6094 Size: 274 80 33 51 1443 516 1889 1712 Cuts at: 6094 96 Size: 138 Fragments arranged by size: 1889 1712 1443 516 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 3269 3301 6096 6128 2078 Size: 32 1159 32 2795 32 2086 Fragments arranged by size: 2795 2086 1159 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2320 Size: 6136 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 2160 3228 4265 2160 Size: 1068 1037 4031 Fragments arranged by size: 4031 1068 1037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI