Restriction map for pFN21AE6250
 (Circular) (Six-base) MAPSORT of: pFN21AE6250.seq  Check: 6559  from: 1  to: 5479



pFN21AE6250  5479 bp

 With 182 enzymes: SgfI * 

                        March 18, 2015 14:50  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2421    3289     278 
   Size:         53      83     186     936     885     868    2468

  Fragments arranged by size: 

                2468     936     885     868     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2822    1937 
   Size:        885    4594
 
Acc65I G'GTAC_C

Cuts at:   2800    2800 
   Size:       5479
 
AclI AA'CG_TT

Cuts at:   1563    3606    3979    1563 
   Size:       2043     373    3063

  Fragments arranged by size: 

                3063    2043     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3542    4926    5194    1632 
   Size:       1910    1384     268    1917

  Fragments arranged by size: 

                1917    1910    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5282
 
AflIII A'CryG_T

Cuts at:   1203    2658    4378    5355    1203 
   Size:       1455    1720     977    1327

  Fragments arranged by size: 

                1720    1455    1327     977
 
AgeI A'CCGG_T

Cuts at:   4310    4310 
   Size:       5479
 
AhdI GACnn_n'nnGTC

Cuts at:   4208    4208 
   Size:       5479
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5479
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5479
 
AlwNI CAG_nnn'CTG

Cuts at:   3222    4794    3222 
   Size:       1572    3907
 
ApaI G_GGCC'C

Cuts at:   2017    2017 
   Size:       5479
 
ApaLI G'TGCA_C

Cuts at:   3536    4692    3536 
   Size:       1156    4323
 
ApoI r'AATT_y

Cuts at:   1493    2788    3014    3166    5275    1493 
   Size:       1295     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1295     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4033     160 
   Size:        872    3001    1606

  Fragments arranged by size: 

                3001    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5479
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2166    2531    2804    1870 
   Size:         73     223     365     273    4545

  Fragments arranged by size: 

                4545     365     273     223      73
 
BamHI G'GATC_C

Cuts at:   2809    3176    2809 
   Size:        367    5112
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2800    4255     618 
   Size:        325     217     627    1013    1455    1842

  Fragments arranged by size: 

                1842    1455    1013     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2017    2050    2165    2798    1391 
   Size:        626      33     115     633    4072

  Fragments arranged by size: 

                4072     633     626     115      33
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4508
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3670    3704    1903 
   Size:         34      50      34    1649      34    3678

  Fragments arranged by size: 

                3678    1649      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3371    4581    3371 
   Size:       1210    4269
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5479
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4090     136 
   Size:        107     122      71     631     605    2418    1525

  Fragments arranged by size: 

                2418    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5474    5474 
   Size:       5479
 
BlpI GC'TnA_GC

Cuts at:   2896    2896 
   Size:       5479
 
Bme1580I G_kGCm'C

Cuts at:   1790    2017    3540    4696    1790 
   Size:        227    1523    1156    2573

  Fragments arranged by size: 

                2573    1523    1156     227
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5479
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3210    4168     442 
   Size:        416      53      83     399      24    1793     958    1753

  Fragments arranged by size: 

                1793    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5479
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2224    2224 
   Size:       5479
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2415    4139    1239 
   Size:       1176    1724    2579

  Fragments arranged by size: 

                2579    1724    1176
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3604    4469    4767    3604 
   Size:        865     298    4316

  Fragments arranged by size: 

                4316     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2212    4142     882 
   Size:        617     713    1930    2219

  Fragments arranged by size: 

                2219    1930     713     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2566    3270     493 
   Size:        711    1362     704    2702

  Fragments arranged by size: 

                2702    1362     711     704
 
BsaBI GATnn'nnATC

Cuts at:   5473    5473 
   Size:       5479
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2418    2681    3286    3668 
   Size:         53      83     186     936     885     263     605     382

Cuts at:   3668    4303     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     885     635     605     382     263     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   3912    4310    4584    4731    5142    3912 
   Size:        398     274     147     411    4249

  Fragments arranged by size: 

                4249     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2602    2632    1178 
   Size:         30     165      30    1199      30    4025

  Fragments arranged by size: 

                4025    1199     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2623    1186 
   Size:       1437    4042
 
BseYI C'CCAG_C

Cuts at:   2029    2063    2752    4682    5178    2029 
   Size:         34     689    1930     496    2330

  Fragments arranged by size: 

                2330    1930     689     496      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2162    1163 
   Size:        573     426    4480

  Fragments arranged by size: 

                4480     573     426
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2929    3690    4718    1540 
   Size:        275     186     928     761    1028    2301

  Fragments arranged by size: 

                2301    1028     928     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3540    3625    4696    3540 
   Size:         85    1071    4323

  Fragments arranged by size: 

                4323    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2990    3083    2990 
   Size:         93    5386
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2017    2050    2165    2798    3540    3625    4696 
   Size:        399     227      33     115     633     742      85    1071

Cuts at:   4696    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     633     399     227     115      85
                  33
 
BspEI T'CCGG_A

Cuts at:   5142    5142 
   Size:       5479
 
BspHI T'CATG_A

Cuts at:   3368    5098    3368 
   Size:       1730    3749
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2834     844 
   Size:       1990    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3366    1394 
   Size:        429    1543    3507

  Fragments arranged by size: 

                3507    1543     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2703    3974    4148      65 
   Size:       1183    1455    1271     174    1396

  Fragments arranged by size: 

                1455    1396    1271    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4123    4310    1700 
   Size:        110    2313     187    2869

  Fragments arranged by size: 

                2869    2313     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5479
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5479
 
BssSI C'ACGA_G

Cuts at:   3539    4551    3539 
   Size:       1012    4467
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5479
 
BstBI TT'CG_AA

Cuts at:   4283    4283 
   Size:       5479
 
BstEII G'GTnAC_C

Cuts at:   1184    2170    1184 
   Size:        986    4493
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2752    2752 
   Size:       5479
 
BstYI r'GATC_y

Cuts at:   1972    2809    3176    3561    3578    5030    5116    5128    5474 
   Size:        837     367     385      17    1452      86      12     346

Cuts at:   5474    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     837     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2149     513 
   Size:        552    1084    3843

  Fragments arranged by size: 

                3843    1084     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4740

  Fragments arranged by size: 

                4740     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2179    2995    3789    3809    2179 
   Size:        816     794      20    3849

  Fragments arranged by size: 

                3849     816     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5479
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5444
 
DraI TTT'AAA

Cuts at:   2783    2783 
   Size:       5479
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2144    4486     809 
   Size:        507     828    2342    1802

  Fragments arranged by size: 

                2342    1802     828     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2926    3815    5444 
   Size:         54    1425      50     275      18    1096     889    1629

Cuts at:   5444       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1096     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2926    1537 
   Size:        275    1114    4090

  Fragments arranged by size: 

                4090    1114     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2093    2098    3409    1353 
   Size:        740       5    1311    3423

  Fragments arranged by size: 

                3423    1311     740       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4066    4440    4586     387 
   Size:       1064     138    2477     374     146    1280

  Fragments arranged by size: 

                2477    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2415    3542    4139    4926    5194    1239 
   Size:        393     783    1127     597     787     268    1524

  Fragments arranged by size: 

                1524    1127     787     783     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2014    2313    2344    2014 
   Size:        299      31    5149

  Fragments arranged by size: 

                5149     299      31
 
EcoRI G'AATT_C

Cuts at:   2788    3166    2788 
   Size:        378    5101
 
FspI TGC'GCA

Cuts at:   3985    3985 
   Size:       5479
 
HaeII r_GCGC'y

Cuts at:   1678    2236    4626    1678 
   Size:        558    2390    2531

  Fragments arranged by size: 

                2531    2390     558
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2204    2236    2502    2534    4120 
   Size:         32     135      32     632      32     266      32    1586

Cuts at:   4120    4152    4194    4226    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1586     632     266     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2823    3069     669 
   Size:        988     281     885     246    3079

  Fragments arranged by size: 

                3079     988     885     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5479
 
HpaI GTT'AAC

Cuts at:   3069    3069 
   Size:       5479
 
KpnI G_GTAC'C

Cuts at:   2804    2804 
   Size:       5479
 
MfeI C'AATT_G

Cuts at:   3078    3078 
   Size:       5479
 
MluI A'CGCG_T

Cuts at:   2658    5355    2658 
   Size:       2697    2782
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4593    4777    1305 
   Size:       3288     184    2007

  Fragments arranged by size: 

                3288    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3657

  Fragments arranged by size: 

                3657    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3437    3796    3955     518 
   Size:        648    2271     359     159    2042

  Fragments arranged by size: 

                2271    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2895    3572    4720    4965    1606 
   Size:       1289     677    1148     245    2120

  Fragments arranged by size: 

                2120    1289    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2149     513 
   Size:        552    1084    3843

  Fragments arranged by size: 

                3843    1084     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5479
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5479
 
NotI GC'GGCC_GC

Cuts at:   2926    2926 
   Size:       5479
 
NspI r_CATG'y

Cuts at:   1132    1229    2837    4382    1132 
   Size:         97    1608    1545    2229

  Fragments arranged by size: 

                2229    1608    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5479
 
PciI A'CATG_T

Cuts at:   4378    4378 
   Size:       5479
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2202    1108 
   Size:       1094    4385
 
PmeI GTTT'AAAC

Cuts at:   2783    2783 
   Size:       5479
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3548    3580    5061    5093    3548 
   Size:         32    1481      32    3934

  Fragments arranged by size: 

                3934    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2313    2344    2313 
   Size:         31    5448
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5479
 
PsiI TTA'TAA

Cuts at:   3049    3049 
   Size:       5479
 
PspOMI G'GGCC_C

Cuts at:   2013    2013 
   Size:       5479
 
PstI C_TGCA'G

Cuts at:    830    2607    2831     830 
   Size:       1777     224    3478

  Fragments arranged by size: 

                3478    1777     224
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5479
 
SalI G'TCGA_C

Cuts at:   1936    2821    1936 
   Size:        885    4594
 
SanDI GG'GwC_CC

Cuts at:   2344    2344 
   Size:       5479
 
SapI GCTCTTCn'nnn_

Cuts at:   2093    2093 
   Size:       5479
 
SbfI CC_TGCA'GG

Cuts at:   2831    2831 
   Size:       5479
 
ScaI AGT'ACT

Cuts at:   3727    5439    3727 
   Size:       1712    3767
 
SfcI C'TryA_G

Cuts at:    826    1045    2389    2603    2827    3962    4643    4834     826 
   Size:        219    1344     214     224    1135     681     191    1471

  Fragments arranged by size: 

                1471    1344    1135     681     224     219     214     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5479
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5479
 
SmaI CCC'GGG

Cuts at:   1872    2168    2806    1872 
   Size:        296     638    4545

  Fragments arranged by size: 

                4545     638     296
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3583    4484    4746     820 
   Size:        197     926    1640     901     262    1553

  Fragments arranged by size: 

                1640    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3270     493 
   Size:       2777    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5479
 
SphI G_CATG'C

Cuts at:   1132    2837    1132 
   Size:       1705    3774
 
SspI AAT'ATT

Cuts at:      5      52    3403       5 
   Size:         47    3351    2081

  Fragments arranged by size: 

                3351    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5479
 
StyI C'CwwG_G

Cuts at:    513    1065    2149    2283    2745    2918     513 
   Size:        552    1084     134     462     173    3074

  Fragments arranged by size: 

                3074    1084     552     462     173     134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3506    3523    3676    3861    4306     325 
   Size:       1255    1926      17     153     185     445    1498

  Fragments arranged by size: 

                1926    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3725    5437      96 
   Size:        274      80      33      51    1443    1748    1712     138

  Fragments arranged by size: 

                1748    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2602    2634    5439    5471    2602 
   Size:         32    2805      32    2610

  Fragments arranged by size: 

                2805    2610      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5479
 
XbaI T'CTAG_A

Cuts at:   2815    2815 
   Size:       5479
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2071    2071 
   Size:       5479
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5479
 
XmaI C'CCGG_G

Cuts at:   1870    2166    2804    1870 
   Size:        296     638    4545

  Fragments arranged by size: 

                4545     638     296
 
XmnI GAAnn'nnTTC

Cuts at:   3608    3608 
   Size:       5479
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2419    3287     276 
   Size:         53      83     186     936     885     868    2468

  Fragments arranged by size: 

                2468     936     885     868     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SexAI     SfiI     SfoI     SrfI
     SwaI
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