(Linear) (Six-base) MAPSORT of: KIEE6250.seq Check: 3776 from: 1 to: 774 KIEE6250.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AatII G_ACGT'C Cuts at: 0 416 774 Size: 416 358 AflIII A'CryG_T Cuts at: 0 653 774 Size: 653 121 ApaI G_GGCC'C Cuts at: 0 12 774 Size: 12 762 AvaI C'yCGr_G Cuts at: 0 161 526 774 Size: 161 365 248 Fragments arranged by size: 365 248 161 BanII G_rGCy'C Cuts at: 0 12 45 160 774 Size: 12 33 115 614 Fragments arranged by size: 614 115 33 12 Bme1580I G_kGCm'C Cuts at: 0 12 774 Size: 12 762 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 219 774 Size: 219 555 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 774 Size: 410 364 BsaI GGTCTCn'nnnn_ Cuts at: 0 207 774 Size: 207 567 BsaAI yAC'GTr Cuts at: 0 561 774 Size: 561 213 BsaHI Gr'CG_yC Cuts at: 0 413 676 774 Size: 413 263 98 Fragments arranged by size: 413 263 98 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 597 627 774 Size: 597 30 147 Fragments arranged by size: 597 147 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 618 774 Size: 618 156 BseYI C'CCAG_C Cuts at: 0 24 58 747 774 Size: 24 34 689 27 Fragments arranged by size: 689 34 27 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 774 Size: 157 617 Bsp1286I G_dGCh'C Cuts at: 0 12 45 160 774 Size: 12 33 115 614 Fragments arranged by size: 614 115 33 12 BsrDI GCAATG_nn' Cuts at: 0 698 774 Size: 698 76 BstEII G'GTnAC_C Cuts at: 0 165 774 Size: 165 609 BstXI CCAn_nnnn'nTGG Cuts at: 0 747 774 Size: 747 27 BtgI C'CryG_G Cuts at: 0 144 774 Size: 144 630 BtsI GCAGTG_nn' Cuts at: 0 174 774 Size: 174 600 DrdI GACnn_nn'nnGTC Cuts at: 0 139 774 Size: 139 635 EarI CTCTTCn'nnn_ Cuts at: 0 88 93 774 Size: 88 5 681 Fragments arranged by size: 681 88 5 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 774 Size: 410 364 EcoO109I rG'GnC_Cy Cuts at: 0 9 308 339 774 Size: 9 299 31 435 Fragments arranged by size: 435 299 31 9 HaeII r_GCGC'y Cuts at: 0 231 774 Size: 231 543 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 199 231 497 529 774 Size: 199 32 266 32 245 Fragments arranged by size: 266 245 199 32 32 MluI A'CGCG_T Cuts at: 0 653 774 Size: 653 121 NcoI C'CATG_G Cuts at: 0 144 774 Size: 144 630 PflMI CCAn_nnn'nTGG Cuts at: 0 197 774 Size: 197 577 PpuMI rG'GwC_Cy Cuts at: 0 308 339 774 Size: 308 31 435 Fragments arranged by size: 435 308 31 PspOMI G'GGCC_C Cuts at: 0 8 774 Size: 8 766 PstI C_TGCA'G Cuts at: 0 602 774 Size: 602 172 SanDI GG'GwC_CC Cuts at: 0 339 774 Size: 339 435 SapI GCTCTTCn'nnn_ Cuts at: 0 88 774 Size: 88 686 SfcI C'TryA_G Cuts at: 0 384 598 774 Size: 384 214 176 Fragments arranged by size: 384 214 176 SmaI CCC'GGG Cuts at: 0 163 774 Size: 163 611 StyI C'CwwG_G Cuts at: 0 144 278 740 774 Size: 144 134 462 34 Fragments arranged by size: 462 144 134 34 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 597 629 774 Size: 597 32 145 Fragments arranged by size: 597 145 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 66 774 Size: 66 708 XmaI C'CCGG_G Cuts at: 0 161 774 Size: 161 613 ZraI GAC'GTC Cuts at: 0 414 774 Size: 414 360 Enzymes that do cut: AatII AflIII ApaI AvaI BanII Bme1580I BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI Bsp1286I BsrDI BstEII BstXI BtgI BtsI DrdI EarI Eco57MI EcoO109I HaeII Hin4I MluI NcoI PflMI PpuMI PspOMI PstI SanDI SapI SfcI SmaI StyI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmnI