################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MroI MunI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 519 GGCGCC G[GA]^CG[CT]C Alw44I 384 GTGCAC G^TGCAC ApaLI 384 GTGCAC G^TGCAC Asp700I 431 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 519 GGCGCC GGCGC^C BbvCI 1153 GCTGAGG CCTCAGC(-5/-2) BglI 1040 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1131 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC BsaHI 519 GGCGCC G[GA]^CG[CT]C BssSI 349 CTCGTG CACGAG(-5/-1) BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1131 GCTGAGC GC^T[ACGT]AGC CfoI 520 GCGC GCG^C EarI 256 CTCTTC CTCTTC(1/4) FauI 788 CCCGC CCCGC(4/6) HaeII 519 GGCGCC [GA]GCGC^[CT] HgaI 660 GCGTC GACGC(5/10) HhaI 520 GCGC GCG^C Hin1I 519 GGCGCC G[GA]^CG[CT]C HinP1I 520 GCGC G^CGC HincII 748 GTTGAC GT[CT]^[GA]AC HindII 748 GTTGAC GT[CT]^[GA]AC KasI 519 GGCGCC G^GCGCC Ksp632I 256 CTCTTC CTCTTC(1/4) MseI 688 TTAA T^TAA MslI 1080 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 536 CAGCTG C[AC]G^C[GT]G NarI 519 GGCGCC GG^CGCC NcoI 924 CCATGG C^CATGG NdeI 812 CATATG CA^TATG PflMI 1023 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 631 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 536 CAGCTG CAG^CTG SacI 64 GAGCTC GAGCT^C SapI 255 GCTCTTC GCTCTTC(1/4) SexAI 773 ACCTGGT A^CC[AT]GGT SfoI 519 GGCGCC GGC^GCC SmaI 188 CCCGGG CCC^GGG SmlI 1282 CTTGAG C^T[CT][GA]AG SphI 1127 GCATGC GCATG^C StuI 1157 AGGCCT AGG^CCT TaqI 650 TCGA T^CGA Tru9I 688 TTAA T^TAA TthHB8I 650 TCGA T^CGA Van91I 1023 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 188 CCCGGG C^CCGGG XmaI 188 CCCGGG C^CCGGG XmnI 431 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 202 GTAGAC GT^[AC][GT]AC 610 GTAGAC AciI 249 CCGC CCGC(-3/-1) 789 CCGC AcsI 362 GAATTC [GA]^AATT[CT] 760 GAATTC ApoI 362 GAATTC [GA]^AATT[CT] 760 GAATTC AvaI 188 CCCGGG C^[CT]CG[GA]G 311 CCCGAG AvaII 357 GGACC G^G[AT]CC 1173 GGACC BsaI 1097 GAGACC GGTCTC(1/5) 391 GGTCTC BseRI 131 GAGGAG GAGGAG(10/8) 1297 GAGGAG BsmBI 466 CGTCTC CGTCTC(1/5) 163 GAGACG BsoBI 188 CCCGGG C^[CT]CG[GA]G 311 CCCGAG DpnII 339 GATC ^GATC 870 GATC DsaI 924 CCATGG C^C[GA][CT]GG 957 CCGTGG EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1281 CCTTGAGAAGG EcoRI 362 GAATTC G^AATTC 760 GAATTC EcoT14I 924 CCATGG C^C[AT][AT]GG 1106 CCAAGG HapII 189 CCGG C^CGG 517 CCGG HinfI 139 GATTC G^A[ACGT]TC 1312 GATTC HpaII 189 CCGG C^CGG 517 CCGG MboI 339 GATC ^GATC 870 GATC MspI 189 CCGG C^CGG 517 CCGG NdeII 339 GATC ^GATC 870 GATC NspI 180 GCATGT [GA]CATG^[CT] 1127 GCATGC Sau3AI 339 GATC ^GATC 870 GATC SfcI 539 CTGTAG C^T[GA][CT]AG 860 CTGTAG StyI 924 CCATGG C^C[AT][AT]GG 1106 CCAAGG TfiI 139 GATTC G^A[AT]TC 1312 GATTC XcmI 414 CCACAGCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1019 CCATCCAGTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AfaI AluI AspHI BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI EaeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeII MaeIII MboII MluNI MnlI MscI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI