Restriction map for
pFN21AE1371
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DrdI Eam1105I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HinfI HpaI KasI
KpnI MaeI MaeII MfeI MflI
MluI MroI MseI MunI NarI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Van91I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 470 CGCG CG^CG
AlwI 57 GATCC GGATC(4/5)
AlwNI 37 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 197 AGCGCT AGC^GCT
ApaI 241 GGGCCC GGGCC^C
Asp700I 231 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 574 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BpmI 154 CTGGAG CTGGAG(16/14)
BsaBI 57 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 345 CATTGC GCAATG(2/0)
BsrFI 301 GCCGGC [GA]^CCGG[CT]
BstAPI 279 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 567 GGTGACC G^GT[ACGT]ACC
BstPI 567 GGTGACC G^GT[ACGT]ACC
BstUI 470 CGCG CG^CG
Cfr10I 301 GCCGGC [GA]^CCGG[CT]
DraIII 99 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 171 GAAGAG CTCTTC(1/4)
Eco47III 197 AGCGCT AGC^GCT
EcoNI 245 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 567 GGTGACC G^GT[ACGT]ACC
HincII 553 GTCAAC GT[CT]^[GA]AC
HindII 553 GTCAAC GT[CT]^[GA]AC
Ksp632I 171 GAAGAG CTCTTC(1/4)
KspI 469 CCGCGG CCGC^GG
MaeIII 568 GTGAC ^GT[ACGT]AC
MamI 57 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 561 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 470 CGCG CG^CG
NaeI 301 GCCGGC GCC^GGC
NcoI 191 CCATGG C^CATGG
NgoMIV 301 GCCGGC G^CCGGC
NotI 420 GCGGCCGC GC^GGCCGC
PflFI 574 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 86 CAGCTG CAG^CTG
SacII 469 CCGCGG CCGC^GG
SfiI 18 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I 568 GTGAC ^GT[GC]AC
Tsp509I 256 AATT ^AATT
Tth111I 574 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 102 CCAGTGGTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 231 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 45 GGCGTC G[GA]^CG[CT]C
393 GACGCC
AluI 87 AGCT AG^CT
233 AGCT
BbvI 447 GCAGC GCAGC(8/12)
514 GCTGC
BglI 19 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
334 GCCTGCCTGGC
BsaHI 45 GGCGTC G[GA]^CG[CT]C
393 GACGCC
BseRI 505 GAGGAG GAGGAG(10/8)
508 GAGGAG
BsiEI 421 CGGCCG CG[GA][CT]^CG
479 CGGCCG
BsrBI 351 GAGCGG CCGCTC(-3/-3)
418 GAGCGG
BsrI 366 ACTGG ACTGG(1/-1)
102 CCAGT
BstF5I 321 CATCC GGATG(2/0)
561 CATCC
EagI 421 CGGCCG C^GGCCG
479 CGGCCG
EclXI 421 CGGCCG C^GGCCG
479 CGGCCG
Eco52I 421 CGGCCG C^GGCCG
479 CGGCCG
EcoT14I 191 CCATGG C^C[AT][AT]GG
324 CCTTGG
FokI 321 CATCC GGATG(9/13)
561 CATCC
HgaI 393 GACGC GACGC(5/10)
46 GCGTC
Hin1I 45 GGCGTC G[GA]^CG[CT]C
393 GACGCC
MboII 171 GAAGA GAAGA(8/7)
177 GAAGA
MspA1I 86 CAGCTG C[AC]G^C[GT]G
469 CCGCGG
PpuMI 531 GGGACCC [GA]G^G[AT]CC[CT]
543 AGGTCCC
StyI 191 CCATGG C^C[AT][AT]GG
324 CCTTGG
TaqI 11 TCGA T^CGA
401 TCGA
TseI 447 GCAGC G^C[AT]GC
514 GCTGC
TspRI 103 CAGTG CA[GC]TG[ACGT][ACGT]^
388 CAGTG
TthHB8I 11 TCGA T^CGA
401 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AvaI AvaII BalI
BanI BanII BcnI BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmaI XmaCI XmaI