(Circular) (Six-base) MAPSORT of: pFN21AE1371.seq Check: 5079 from: 1 to: 5290 pFN21AE1371 5290 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3100 278 Size: 53 83 186 936 1564 2468 Fragments arranged by size: 2468 1564 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2633 1937 Size: 696 4594 Acc65I G'GTAC_C Cuts at: 2611 2611 Size: 5290 AclI AA'CG_TT Cuts at: 1563 3417 3790 1563 Size: 1854 373 3063 Fragments arranged by size: 3063 1854 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3353 4737 5005 1632 Size: 1721 1384 268 1917 Fragments arranged by size: 1917 1721 1384 268 AfeI AGC'GCT Cuts at: 2204 2204 Size: 5290 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5093 AflIII A'CryG_T Cuts at: 1203 4189 5166 1203 Size: 2986 977 1327 Fragments arranged by size: 2986 1327 977 AgeI A'CCGG_T Cuts at: 4121 4121 Size: 5290 AhdI GACnn_n'nnGTC Cuts at: 4019 4019 Size: 5290 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5290 AlwNI CAG_nnn'CTG Cuts at: 2047 3033 4605 2047 Size: 986 1572 2732 Fragments arranged by size: 2732 1572 986 ApaI G_GGCC'C Cuts at: 2250 2250 Size: 5290 ApaLI G'TGCA_C Cuts at: 3347 4503 3347 Size: 1156 4134 ApoI r'AATT_y Cuts at: 1493 2599 2825 2977 5086 1493 Size: 1106 226 152 2109 1697 Fragments arranged by size: 2109 1697 1106 226 152 AseI AT'TA_AT Cuts at: 160 1032 3844 160 Size: 872 2812 1606 Fragments arranged by size: 2812 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5290 AvaI C'yCGr_G Cuts at: 1870 1943 2169 2418 2542 2615 1870 Size: 73 226 249 124 73 4545 Fragments arranged by size: 4545 249 226 124 73 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2104 2137 2104 Size: 33 5257 BamHI G'GATC_C Cuts at: 2620 2987 2620 Size: 367 4923 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2299 2441 2611 4066 Size: 325 217 627 333 179 142 170 1455 Cuts at: 4066 618 Size: 1842 Fragments arranged by size: 1842 1455 627 333 325 217 179 170 142 BanII G_rGCy'C Cuts at: 1391 2192 2250 2412 2609 1391 Size: 801 58 162 197 4072 Fragments arranged by size: 4072 801 197 162 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3481 3515 1903 Size: 34 1544 34 3678 Fragments arranged by size: 3678 1544 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3182 4392 3182 Size: 1210 4080 BclI T'GATC_A Cuts at: 1548 1548 Size: 5290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 2345 3901 Size: 107 122 71 631 605 358 315 1556 Cuts at: 3901 136 Size: 1525 Fragments arranged by size: 1556 1525 631 605 358 315 122 107 71 BglII A'GATC_T Cuts at: 5285 5285 Size: 5290 BlpI GC'TnA_GC Cuts at: 2707 2707 Size: 5290 Bme1580I G_kGCm'C Cuts at: 1790 2250 2304 3351 4507 1790 Size: 460 54 1047 1156 2573 Fragments arranged by size: 2573 1156 1047 460 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5290 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2101 3021 3979 Size: 416 53 83 399 24 684 920 958 Cuts at: 3979 442 Size: 1753 Fragments arranged by size: 1753 958 920 684 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 3950 1239 Size: 941 1770 2579 Fragments arranged by size: 2579 1770 941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3415 4280 4578 3415 Size: 865 298 4127 Fragments arranged by size: 4127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3953 882 Size: 617 2454 2219 Fragments arranged by size: 2454 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3081 493 Size: 711 1877 2702 Fragments arranged by size: 2702 1877 711 BsaBI GATnn'nnATC Cuts at: 2066 5284 2066 Size: 3218 2072 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2051 2399 3097 3479 Size: 53 83 186 936 518 348 698 382 Cuts at: 3479 4114 275 Size: 635 1451 Fragments arranged by size: 1451 936 698 635 518 382 348 186 83 53 BsaWI w'CCGG_w Cuts at: 3723 4121 4395 4542 4953 3723 Size: 398 274 147 411 4060 Fragments arranged by size: 4060 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5065 Fragments arranged by size: 5065 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 2528 1186 Size: 1339 3 3948 Fragments arranged by size: 3948 1339 3 BseYI C'CCAG_C Cuts at: 2093 2205 2459 2504 4493 4989 2093 Size: 112 254 45 1989 496 2394 Fragments arranged by size: 2394 1989 496 254 112 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4717 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2429 2487 2740 3501 4529 1540 Size: 275 186 428 58 253 761 1028 2301 Fragments arranged by size: 2301 1028 761 428 275 253 186 58 BsiHKAI G_wGCw'C Cuts at: 3351 3436 4507 3351 Size: 85 1071 4134 Fragments arranged by size: 4134 1071 85 BsmI GAATG_Cn' Cuts at: 2801 2894 2801 Size: 93 5197 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2250 2304 2412 2609 3351 3436 Size: 399 402 58 54 108 197 742 85 Cuts at: 3436 4507 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 197 108 85 58 54 BspEI T'CCGG_A Cuts at: 4953 4953 Size: 5290 BspHI T'CATG_A Cuts at: 3179 4909 3179 Size: 1730 3560 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2645 844 Size: 1801 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2358 2425 3177 1394 Size: 429 535 67 752 3507 Fragments arranged by size: 3507 752 535 429 67 BsrDI GCAATG_nn' Cuts at: 65 1248 2349 3785 3959 65 Size: 1183 1101 1436 174 1396 Fragments arranged by size: 1436 1396 1183 1101 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2306 3934 4121 1700 Size: 110 496 1628 187 2869 Fragments arranged by size: 2869 1628 496 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5290 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5290 BssSI C'ACGA_G Cuts at: 3350 4362 3350 Size: 1012 4278 BstAPI GCAn_nnn'nTGC Cuts at: 794 2290 794 Size: 1496 3794 BstBI TT'CG_AA Cuts at: 4094 4094 Size: 5290 BstEII G'GTnAC_C Cuts at: 1184 2572 1184 Size: 1388 3902 BstYI r'GATC_y Cuts at: 1972 2620 2987 3372 3389 4841 4927 4939 5285 Size: 648 367 385 17 1452 86 12 346 Cuts at: 5285 1972 Size: 1977 Fragments arranged by size: 1977 1452 648 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2196 2229 2474 513 Size: 552 939 192 33 245 3329 Fragments arranged by size: 3329 939 552 245 192 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4551 Fragments arranged by size: 4551 683 56 BtsI GCAGTG_nn' Cuts at: 2399 2806 3600 3620 2399 Size: 407 794 20 4069 Fragments arranged by size: 4069 794 407 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5290 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2311 2346 529 Size: 35 1747 35 3473 Fragments arranged by size: 3473 1747 35 35 DraI TTT'AAA Cuts at: 2594 2594 Size: 5290 DraIII CAC_nnn'GTG Cuts at: 2109 2109 Size: 5290 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4297 809 Size: 507 2981 1802 Fragments arranged by size: 2981 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2319 2346 Size: 54 1425 50 275 18 255 234 27 Cuts at: 2346 2426 2484 2737 3626 5255 8 Size: 80 58 253 889 1629 43 Fragments arranged by size: 1629 1425 889 275 255 253 234 80 58 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2426 2484 2737 1537 Size: 275 614 58 253 4090 Fragments arranged by size: 4090 614 275 253 58 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3220 1353 Size: 818 1049 3423 Fragments arranged by size: 3423 1049 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3877 4251 4397 387 Size: 1064 138 2288 374 146 1280 Fragments arranged by size: 2288 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 3353 3950 4737 5005 1239 Size: 393 548 1173 597 787 268 1524 Fragments arranged by size: 1524 1173 787 597 548 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2254 2254 Size: 5290 EcoO109I rG'GnC_Cy Cuts at: 2247 2537 2549 2247 Size: 290 12 4988 Fragments arranged by size: 4988 290 12 EcoRI G'AATT_C Cuts at: 2599 2977 2599 Size: 378 4912 FspI TGC'GCA Cuts at: 3796 3796 Size: 5290 HaeII r_GCGC'y Cuts at: 1678 2048 2101 2206 4437 1678 Size: 370 53 105 2231 2531 Fragments arranged by size: 2531 2231 370 105 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2430 2462 3931 3963 4005 Size: 32 135 32 858 32 1469 32 42 Cuts at: 4005 4037 1373 Size: 32 2626 Fragments arranged by size: 2626 1469 858 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2560 2634 2880 669 Size: 988 281 622 74 246 3079 Fragments arranged by size: 3079 988 622 281 246 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 5290 HpaI GTT'AAC Cuts at: 2880 2880 Size: 5290 KpnI G_GTAC'C Cuts at: 2615 2615 Size: 5290 MfeI C'AATT_G Cuts at: 2889 2889 Size: 5290 MluI A'CGCG_T Cuts at: 5166 5166 Size: 5290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4404 4588 1305 Size: 3099 184 2007 Fragments arranged by size: 3099 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2087 2321 2348 10 Size: 54 1425 343 255 234 27 2952 Fragments arranged by size: 2952 1425 343 255 234 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2570 3248 3607 3766 518 Size: 648 1404 678 359 159 2042 Fragments arranged by size: 2042 1404 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2093 2476 2706 3383 4531 4776 1606 Size: 487 383 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 487 383 245 230 NaeI GCC'GGC Cuts at: 2308 2308 Size: 5290 NcoI C'CATG_G Cuts at: 513 1065 2004 2196 513 Size: 552 939 192 3607 Fragments arranged by size: 3607 939 552 192 NdeI CA'TA_TG Cuts at: 387 387 Size: 5290 NgoMIV G'CCGG_C Cuts at: 2306 2306 Size: 5290 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5290 NotI GC'GGCC_GC Cuts at: 2426 2737 2426 Size: 311 4979 NspI r_CATG'y Cuts at: 1132 1229 2648 4193 1132 Size: 97 1419 1545 2229 Fragments arranged by size: 2229 1545 1419 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5290 PciI A'CATG_T Cuts at: 4189 4189 Size: 5290 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5290 PmeI GTTT'AAAC Cuts at: 2594 2594 Size: 5290 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3359 3391 4872 4904 3359 Size: 32 1481 32 3745 Fragments arranged by size: 3745 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2537 2549 2537 Size: 12 5278 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5290 PsiI TTA'TAA Cuts at: 2860 2860 Size: 5290 PspOMI G'GGCC_C Cuts at: 2246 2246 Size: 5290 PstI C_TGCA'G Cuts at: 830 2642 830 Size: 1812 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5290 PvuII CAG'CTG Cuts at: 2093 2093 Size: 5290 SacII CC_GC'GG Cuts at: 2477 2477 Size: 5290 SalI G'TCGA_C Cuts at: 1936 2632 1936 Size: 696 4594 SanDI GG'GwC_CC Cuts at: 2537 2537 Size: 5290 SbfI CC_TGCA'GG Cuts at: 2642 2642 Size: 5290 ScaI AGT'ACT Cuts at: 3538 5250 3538 Size: 1712 3578 SfcI C'TryA_G Cuts at: 826 1045 2638 3773 4454 4645 826 Size: 219 1593 1135 681 191 1471 Fragments arranged by size: 1593 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2030 2030 Size: 5290 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5290 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5290 SmaI CCC'GGG Cuts at: 1872 2171 2420 2544 2617 1872 Size: 299 249 124 73 4545 Fragments arranged by size: 4545 299 249 124 73 SmlI C'TyrA_G Cuts at: 820 1017 1943 3394 4295 4557 820 Size: 197 926 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3081 493 Size: 2588 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5290 SphI G_CATG'C Cuts at: 1132 2648 1132 Size: 1516 3774 SspI AAT'ATT Cuts at: 5 52 3214 5 Size: 47 3162 2081 Fragments arranged by size: 3162 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5290 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 2329 2729 513 Size: 552 939 192 133 400 3074 Fragments arranged by size: 3074 939 552 400 192 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2120 2591 3317 3334 3487 3672 4117 Size: 1255 540 471 726 17 153 185 445 Cuts at: 4117 325 Size: 1498 Fragments arranged by size: 1498 1255 726 540 471 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2217 3536 5248 Size: 274 80 33 51 1443 240 1319 1712 Cuts at: 5248 96 Size: 138 Fragments arranged by size: 1712 1443 1319 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5250 5282 5250 Size: 32 5258 Tth111I GACn'n_nGTC Cuts at: 1316 2582 1316 Size: 1266 4024 XbaI T'CTAG_A Cuts at: 2626 2626 Size: 5290 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2114 2114 Size: 5290 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5290 XmaI C'CCGG_G Cuts at: 1870 2169 2418 2542 2615 1870 Size: 299 249 124 73 4545 Fragments arranged by size: 4545 299 249 124 73 XmnI GAAnn'nnTTC Cuts at: 2240 3419 2240 Size: 1179 4111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3098 276 Size: 53 83 186 936 1564 2468 Fragments arranged by size: 2468 1564 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfoI SrfI SwaI