################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccIII AclI AflII Aor51HI AscI AseI AsnI Asp700I AviII BalI BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BseAI BseRI BsgI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eco47III EcoO65I EcoRI EcoRV FseI FspI HaeII HindIII HpaI KasI MamI MfeI MluI MluNI MroI MscI MslI MunI NarI NcoI NdeI NheI NruI PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 269 GGTACC G^GTACC AcyI 127 GACGCC G[GA]^CG[CT]C AgeI 272 ACCGGT A^CCGGT AhdI 460 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 999 GTGCAC G^TGCAC AocI 309 CCTGAGG CC^T[ACGT]AGG ApaI 338 GGGCCC GGGCC^C ApaLI 999 GTGCAC G^TGCAC Asp718I 269 GGTACC G^GTACC AspEI 460 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 433 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1108 CCTAGG C^CTAGG BanII 338 GGGCCC G[GA]GC[CT]^C BciVI 642 GTATCC GTATCC(6/5) BclI 300 TGATCA T^GATCA BlnI 1108 CCTAGG C^CTAGG BsaHI 127 GACGCC G[GA]^CG[CT]C BspLU11I 1214 ACATGT A^CATGT BsrDI 80 GCAATG GCAATG(2/0) BssSI 43 CTCGTG CACGAG(-5/-1) BstBI 413 TTCGAA TT^CGAA BstXI 1075 CCAAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1112 GGATCT [GA]^GATC[CT] Bsu36I 309 CCTGAGG CC^T[ACGT]AGG CfoI 139 GCGC GCG^C DsaI 156 CCGCGG C^C[GA][CT]GG EagI 153 CGGCCG C^GGCCG Eam1105I 460 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 153 CGGCCG C^GGCCG Eco52I 153 CGGCCG C^GGCCG Eco81I 309 CCTGAGG CC^T[ACGT]AGG EcoNI 771 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1108 CCTAGG C^C[AT][AT]GG EcoT22I 1138 ATGCAT ATGCA^T FbaI 300 TGATCA T^GATCA HhaI 139 GCGC GCG^C Hin1I 127 GACGCC G[GA]^CG[CT]C HinP1I 139 GCGC G^CGC HincII 468 GTCGAC GT[CT]^[GA]AC HindII 468 GTCGAC GT[CT]^[GA]AC HphI 498 GGTGA GGTGA(8/7) KpnI 269 GGTACC GGTAC^C KspI 156 CCGCGG CCGC^GG MflI 1112 GGATCT [GA]^GATC[CT] MseI 1195 TTAA T^TAA NaeI 148 GCCGGC GCC^GGC NgoMIV 148 GCCGGC G^CCGGC NotI 152 GCGGCCGC GC^GGCCGC NsiI 1138 ATGCAT ATGCA^T NspI 1214 ACATGT [GA]CATG^[CT] NspV 413 TTCGAA TT^CGAA PflFI 433 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 272 ACCGGT A^CCGGT PpuMI 874 GGGACCT [GA]G^G[AT]CC[CT] SacII 156 CCGCGG CCGC^GG SalI 468 GTCGAC G^TCGAC SfuI 413 TTCGAA TT^CGAA SmaI 344 CCCGGG CCC^GGG StyI 1108 CCTAGG C^C[AT][AT]GG Tru9I 1195 TTAA T^TAA Tth111I 433 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1112 GGATCT [GA]^GATC[CT] XmaCI 344 CCCGGG C^CCGGG XmaI 344 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 468 GTCGAC GT^[AC][GT]AC 1157 GTAGAC AflIII 206 ACGTGT A^C[GA][CT]GT 1214 ACATGT AlwI 780 GGATC GGATC(4/5) 1112 GGATC AlwNI 304 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 985 CAGAAGCTG AspHI 946 GAGCAC G[AT]GC[AT]^C 999 GTGCAC AvaI 244 CCCGAG C^[CT]CG[GA]G 344 CCCGGG BpmI 72 CTGGAG CTGGAG(16/14) 1124 CTGGAG BsaI 593 GAGACC GGTCTC(1/5) 198 GGTCTC BsaWI 272 ACCGGT [AT]^CCGG[AT] 510 TCCGGT BsiEI 153 CGGCCG CG[GA][CT]^CG 470 CGACCG BsiHKAI 946 GAGCAC G[AT]GC[AT]^C 999 GTGCAC BsoBI 244 CCCGAG C^[CT]CG[GA]G 344 CCCGGG BspMI 596 ACCTGC ACCTGC(4/8) 519 GCAGGT BstF5I 189 CATCC GGATG(2/0) 1163 CATCC EarI 1046 CTCTTC CTCTTC(1/4) 775 GAAGAG FauI 136 CCCGC CCCGC(4/6) 141 GCGGG FokI 189 CATCC GGATG(9/13) 1163 CATCC Ksp632I 1046 CTCTTC CTCTTC(1/4) 775 GAAGAG MaeII 206 ACGT A^CGT 437 ACGT MaeIII 572 GTGAC ^GT[ACGT]AC 1188 GTCAC PstI 517 CTGCAG CTGCA^G 1336 CTGCAG PvuII 289 CAGCTG CAG^CTG 484 CAGCTG Tsp45I 572 GTGAC ^GT[GC]AC 1188 GTCAC TspRI 697 CACTG CA[GC]TG[ACGT][ACGT]^ 1239 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AvaII BanI BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HgaI HinfI HpaII ItaI MaeI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp509I TthHB8I