(Circular) (Six-base) MAPSORT of: pFN21AE6433.seq Check: 6027 from: 1 to: 6046 pFN21AE6433 6046 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2515 2515 Size: 6046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2474 3163 3389 1937 Size: 537 689 226 4594 Fragments arranged by size: 4594 689 537 226 Acc65I G'GTAC_C Cuts at: 2274 3367 2274 Size: 1093 4953 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2858 4109 5493 5761 1632 Size: 1226 1251 1384 268 1917 Fragments arranged by size: 1917 1384 1251 1226 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 2211 3219 4945 5922 1203 Size: 1008 1008 1726 977 1327 Fragments arranged by size: 1726 1327 1008 1008 977 AgeI A'CCGG_T Cuts at: 2277 4877 2277 Size: 2600 3446 AhdI GACnn_n'nnGTC Cuts at: 2470 4775 2470 Size: 2305 3741 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2314 2995 3789 5361 2314 Size: 681 794 1572 2999 Fragments arranged by size: 2999 1572 794 681 ApaI G_GGCC'C Cuts at: 2347 2347 Size: 6046 ApaLI G'TGCA_C Cuts at: 3004 4103 5259 3004 Size: 1099 1156 3791 Fragments arranged by size: 3791 1156 1099 ApoI r'AATT_y Cuts at: 1493 2506 2681 2742 3083 3108 3355 3581 3733 Size: 1013 175 61 341 25 247 226 152 Cuts at: 3733 5842 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1013 341 247 226 175 152 61 25 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2249 2349 3371 1870 Size: 73 306 100 1022 4545 Fragments arranged by size: 4545 1022 306 100 73 AvrII C'CTAG_G Cuts at: 3113 3113 Size: 6046 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2274 2336 3367 4822 Size: 325 217 627 363 124 62 1031 1455 Cuts at: 4822 618 Size: 1842 Fragments arranged by size: 1842 1455 1031 627 363 325 217 124 62 BanII G_rGCy'C Cuts at: 1391 2347 3365 1391 Size: 956 1018 4072 Fragments arranged by size: 4072 1018 956 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2809 3068 3343 928 Size: 971 910 259 275 3631 Fragments arranged by size: 3631 971 910 275 259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2658 3938 5148 2658 Size: 1280 1210 3556 Fragments arranged by size: 3556 1280 1210 BclI T'GATC_A Cuts at: 1548 2305 1548 Size: 757 5289 BfrBI ATG'CAT Cuts at: 3145 3145 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 2347 2855 3008 3184 4107 5263 1790 Size: 557 508 153 176 923 1156 2573 Fragments arranged by size: 2573 1156 923 557 508 176 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3049 3777 4735 Size: 416 53 83 399 24 1632 728 958 Cuts at: 4735 442 Size: 1753 Fragments arranged by size: 1753 1632 958 728 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 3150 4706 1239 Size: 859 1052 1556 2579 Fragments arranged by size: 2579 1556 1052 859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2209 2592 4709 882 Size: 617 710 383 2117 2219 Fragments arranged by size: 2219 2117 710 617 383 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 3853 4235 4870 Size: 53 83 186 936 600 1720 382 635 Cuts at: 4870 275 Size: 1451 Fragments arranged by size: 1720 1451 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2277 2515 4479 4877 5151 5298 5709 2277 Size: 238 1964 398 274 147 411 2614 Fragments arranged by size: 2614 1964 411 398 274 238 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5821 Fragments arranged by size: 5821 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6046 BseYI C'CCAG_C Cuts at: 2244 5249 5745 2244 Size: 3005 496 2545 Fragments arranged by size: 3005 2545 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5473 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2161 2478 3496 4257 5285 1540 Size: 275 186 160 317 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 317 275 186 160 BsiHKAI G_wGCw'C Cuts at: 2955 3008 4107 4192 5263 2955 Size: 53 1099 85 1071 3738 Fragments arranged by size: 3738 1099 1071 85 53 BsmI GAATG_Cn' Cuts at: 2544 2617 2986 3557 3650 2544 Size: 73 369 571 93 4940 Fragments arranged by size: 4940 571 369 93 73 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2347 2855 2955 3008 3184 3365 4107 Size: 399 557 508 100 53 176 181 742 Cuts at: 4107 4192 5263 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 508 399 181 176 100 85 53 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2515 2610 3334 3401 844 Size: 1671 95 724 67 3489 Fragments arranged by size: 3489 1671 724 95 67 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2092 4541 4715 65 Size: 1183 844 2449 174 1396 Fragments arranged by size: 2449 1396 1183 844 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2153 2277 2382 4690 4877 1700 Size: 110 343 124 105 2308 187 2869 Fragments arranged by size: 2869 2308 343 187 124 110 105 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 2048 4106 5118 2048 Size: 2058 1012 2976 Fragments arranged by size: 2976 2058 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6046 BstBI TT'CG_AA Cuts at: 2419 4850 2419 Size: 2431 3615 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstXI CCAn_nnnn'nTGG Cuts at: 3087 3087 Size: 6046 BstYI r'GATC_y Cuts at: 1972 3117 3376 3743 4128 4145 5597 5683 5695 Size: 1145 259 367 385 17 1452 86 12 Cuts at: 5695 6041 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1145 385 367 346 259 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2315 2315 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2161 513 Size: 552 1096 4398 Fragments arranged by size: 4398 1096 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 3562 4356 4376 3562 Size: 794 20 5232 Fragments arranged by size: 5232 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2158 2662 Size: 54 1425 50 275 18 225 103 504 Cuts at: 2662 3493 4382 6011 8 Size: 831 889 1629 43 Fragments arranged by size: 1629 1425 889 831 504 275 225 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2158 3493 1537 Size: 275 346 1335 4090 Fragments arranged by size: 4090 1335 346 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2775 3057 3976 1353 Size: 1422 282 919 3423 Fragments arranged by size: 3423 1422 919 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2270 4633 5007 5153 387 Size: 1064 138 681 2363 374 146 1280 Fragments arranged by size: 2363 1280 1064 681 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2858 3150 4109 4706 5493 5761 Size: 393 466 760 292 959 597 787 268 Cuts at: 5761 1239 Size: 1524 Fragments arranged by size: 1524 959 787 760 597 466 393 292 268 EcoNI CCTnn'n_nnAGG Cuts at: 2780 2780 Size: 6046 EcoO109I rG'GnC_Cy Cuts at: 2110 2344 2880 2110 Size: 234 536 5276 Fragments arranged by size: 5276 536 234 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 5193 1678 Size: 3515 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 2456 2488 2683 Size: 32 135 32 590 32 262 32 195 Cuts at: 2683 2715 2855 2887 4687 4719 4761 4793 1373 Size: 32 140 32 1800 32 42 32 2626 Fragments arranged by size: 2626 1800 590 262 195 140 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2475 3390 3636 669 Size: 988 281 537 915 246 3079 Fragments arranged by size: 3079 988 915 537 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 2278 3371 2278 Size: 1093 4953 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 5160 5344 1305 Size: 927 2928 184 2007 Fragments arranged by size: 2928 2007 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4224 Fragments arranged by size: 4224 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4004 4363 4522 518 Size: 648 2838 359 159 2042 Fragments arranged by size: 2838 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2163 2296 2491 3462 4139 5287 5532 1606 Size: 557 133 195 971 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 677 557 245 195 133 NaeI GCC'GGC Cuts at: 2155 2155 Size: 6046 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5494 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NgoMIV G'CCGG_C Cuts at: 2153 2153 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 2158 3493 2158 Size: 1335 4711 NsiI A_TGCA'T Cuts at: 3147 3147 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 3223 3404 4949 1132 Size: 97 1994 181 1545 2229 Fragments arranged by size: 2229 1994 1545 181 97 PasI CC'CwG_GG Cuts at: 1348 2428 2875 1348 Size: 1080 447 4519 Fragments arranged by size: 4519 1080 447 PciI A'CATG_T Cuts at: 3219 4945 3219 Size: 1726 4320 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2880 2880 Size: 6046 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3036 3616 3036 Size: 580 5466 PspOMI G'GGCC_C Cuts at: 2343 2343 Size: 6046 PstI C_TGCA'G Cuts at: 830 2526 3345 3398 830 Size: 1696 819 53 3478 Fragments arranged by size: 3478 1696 819 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 2296 2491 2296 Size: 195 5851 SacII CC_GC'GG Cuts at: 2164 2164 Size: 6046 SalI G'TCGA_C Cuts at: 1936 2473 3388 1936 Size: 537 915 4594 Fragments arranged by size: 4594 915 537 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2522 2884 3160 3341 3394 4529 5210 Size: 219 1477 362 276 181 53 1135 681 Cuts at: 5210 5401 826 Size: 191 1471 Fragments arranged by size: 1477 1471 1135 681 362 276 219 191 181 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 2351 3373 1872 Size: 479 1022 4545 Fragments arranged by size: 4545 1022 479 SmlI C'TyrA_G Cuts at: 820 1017 1943 4150 5051 5313 820 Size: 197 926 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6046 StyI C'CwwG_G Cuts at: 513 1065 3113 3485 513 Size: 552 2048 372 3074 Fragments arranged by size: 3074 2048 552 372 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2492 4073 4090 4243 4428 4873 325 Size: 1255 912 1581 17 153 185 445 1498 Fragments arranged by size: 1581 1498 1255 912 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3020 4292 6004 Size: 274 80 33 51 1443 1043 1272 1712 Cuts at: 6004 96 Size: 138 Fragments arranged by size: 1712 1443 1272 1043 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2872 2904 6006 6038 2872 Size: 32 3102 32 2880 Fragments arranged by size: 3102 2880 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2441 1316 Size: 1125 4921 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 2349 3371 1870 Size: 479 1022 4545 Fragments arranged by size: 4545 1022 479 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI