Restriction map for pFN21AE6140
 (Circular) (Six-base) MAPSORT of: pFN21AE6140.seq  Check: 6316  from: 1  to: 5215



pFN21AE6140  5215 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:53  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2411    3025     278 
   Size:         53      83     186     936     875     614    2468

  Fragments arranged by size: 

                2468     936     875     614     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2558    1937 
   Size:        621    4594
 
Acc65I G'GTAC_C

Cuts at:   2536    2536 
   Size:       5215
 
AclI AA'CG_TT

Cuts at:   1563    3342    3715    1563 
   Size:       1779     373    3063

  Fragments arranged by size: 

                3063    1779     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2304    3278    4662    4930    1632 
   Size:        672     974    1384     268    1917

  Fragments arranged by size: 

                1917    1384     974     672     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5018
 
AflIII A'CryG_T

Cuts at:   1203    2479    4114    5091    1203 
   Size:       1276    1635     977    1327

  Fragments arranged by size: 

                1635    1327    1276     977
 
AgeI A'CCGG_T

Cuts at:   4046    4046 
   Size:       5215
 
AhdI GACnn_n'nnGTC

Cuts at:   2407    3944    2407 
   Size:       1537    3678
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5215
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2186    1184 
   Size:       1002    4213
 
AlwNI CAG_nnn'CTG

Cuts at:   2958    4530    2958 
   Size:       1572    3643
 
ApaLI G'TGCA_C

Cuts at:   3272    4428    3272 
   Size:       1156    4059
 
ApoI r'AATT_y

Cuts at:   1493    2524    2750    2902    5011    1493 
   Size:       1031     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1031     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3769     160 
   Size:        872    2737    1606

  Fragments arranged by size: 

                2737    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5215
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2540    1870 
   Size:         73     597    4545

  Fragments arranged by size: 

                4545     597      73
 
AvrII C'CTAG_G

Cuts at:   2236    2236 
   Size:       5215
 
BamHI G'GATC_C

Cuts at:   2545    2912    2545 
   Size:        367    4848
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2351    2536    3991     618 
   Size:        325     217     627     564     185    1455    1842

  Fragments arranged by size: 

                1842    1455     627     564     325     217     185
 
BanII G_rGCy'C

Cuts at:   1391    2190    2534    1391 
   Size:        799     344    4072

  Fragments arranged by size: 

                4072     799     344
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2292     928 
   Size:        971     393    3851

  Fragments arranged by size: 

                3851     971     393
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3406    3440    1903 
   Size:         34    1469      34    3678

  Fragments arranged by size: 

                3678    1469      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3107    4317    3107 
   Size:       1210    4005
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5215
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2108    3826     136 
   Size:        107     122      71     631     605     436    1718    1525

  Fragments arranged by size: 

                1718    1525     631     605     436     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5210    5210 
   Size:       5215
 
BlpI GC'TnA_GC

Cuts at:   2632    2632 
   Size:       5215
 
Bme1580I G_kGCm'C

Cuts at:   1790    2104    2458    3276    4432    1790 
   Size:        314     354     818    1156    2573

  Fragments arranged by size: 

                2573    1156     818     354     314
 
BmgBI CAC'GTC

Cuts at:   1318    2266    2398    2482    1318 
   Size:        948     132      84    4051

  Fragments arranged by size: 

                4051     948     132      84
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2946    3904     442 
   Size:        416      53      83     399      24    1529     958    1753

  Fragments arranged by size: 

                1753    1529     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5215
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3875    1239 
   Size:       2636    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2138    2138 
   Size:       5215
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3340    4205    4503    3340 
   Size:        865     298    4052

  Fragments arranged by size: 

                4052     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3878     882 
   Size:        617    2379    2219

  Fragments arranged by size: 

                2379    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3006     493 
   Size:        711    1802    2702

  Fragments arranged by size: 

                2702    1802     711
 
BsaBI GATnn'nnATC

Cuts at:   2510    5209    2510 
   Size:       2699    2516
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2244    2408    2461    3022 
   Size:         53      83     186     936     711     164      53     561

Cuts at:   3022    3404    4039     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                1451     936     711     635     561     382     186     164
                  83      53      53
 
BsaWI w'CCGG_w

Cuts at:   2496    3648    4046    4320    4467    4878    2496 
   Size:       1152     398     274     147     411    2833

  Fragments arranged by size: 

                2833    1152     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4990

  Fragments arranged by size: 

                4990     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2059    2062    2074    2077    1186 
   Size:        873       3      12       3    4324

  Fragments arranged by size: 

                4324     873      12       3       3
 
BseYI C'CCAG_C

Cuts at:   2200    4418    4914    2200 
   Size:       2218     496    2501

  Fragments arranged by size: 

                2501    2218     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4642
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2665    3426    4454    1540 
   Size:        275     186     664     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     664     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3276    3361    4432    3276 
   Size:         85    1071    4059

  Fragments arranged by size: 

                4059    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2726    2819    2726 
   Size:         93    5122
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2235    2235 
   Size:       5215
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2104    2190    2458    2534    3276    3361    4432 
   Size:        399     314      86     268      76     742      85    1071

Cuts at:   4432    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     399     314     268      86      85
                  76
 
BspEI T'CCGG_A

Cuts at:   2496    4878    2496 
   Size:       2382    2833
 
BspHI T'CATG_A

Cuts at:   2358    3104    4834    2358 
   Size:        746    1730    2739

  Fragments arranged by size: 

                2739    1730     746
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2570     844 
   Size:       1726    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2036    3102    1394 
   Size:        429     213    1066    3507

  Fragments arranged by size: 

                3507    1066     429     213
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3710    3884      65 
   Size:       1183    2462     174    1396

  Fragments arranged by size: 

                2462    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3859    4046    1700 
   Size:        110    2049     187    2869

  Fragments arranged by size: 

                2869    2049     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5215
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5215
 
BssSI C'ACGA_G

Cuts at:   3275    4287    3275 
   Size:       1012    4203
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5215
 
BstBI TT'CG_AA

Cuts at:   4019    4019 
   Size:       5215
 
BstEII G'GTnAC_C

Cuts at:   1184    2413    1184 
   Size:       1229    3986
 
BstYI r'GATC_y

Cuts at:   1972    2545    2912    3297    3314    4766    4852    4864    5210 
   Size:        573     367     385      17    1452      86      12     346

Cuts at:   5210    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     573     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2124     513 
   Size:        552    1059    3604

  Fragments arranged by size: 

                3604    1059     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4476

  Fragments arranged by size: 

                4476     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2731    3525    3545    2731 
   Size:        794      20    4401

  Fragments arranged by size: 

                4401     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5215
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5180
 
DraI TTT'AAA

Cuts at:   2519    2519 
   Size:       5215
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2294    2354    4222     809 
   Size:        507     978      60    1868    1802

  Fragments arranged by size: 

                1868    1802     978     507      60
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2662    3551    5180 
   Size:         54    1425      50     275      18     832     889    1629

Cuts at:   5180       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     832     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2662    1537 
   Size:        275     850    4090

  Fragments arranged by size: 

                4090     850     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2026    2062    2080    3145    1353 
   Size:        673      36      18    1065    3423

  Fragments arranged by size: 

                3423    1065     673      36      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3802    4176    4322     387 
   Size:       1064     138    2213     374     146    1280

  Fragments arranged by size: 

                2213    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2304    3278    3875    4662    4930    1239 
   Size:        393     672     974     597     787     268    1524

  Fragments arranged by size: 

                1524     974     787     672     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2116    2134    2116 
   Size:         18    5197
 
EcoRI G'AATT_C

Cuts at:   2524    2902    2524 
   Size:        378    4837
 
FspI TGC'GCA

Cuts at:   3721    3721 
   Size:       5215
 
HaeII r_GCGC'y

Cuts at:   1678    4362    1678 
   Size:       2684    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2393    2407    2425    2439    3856 
   Size:         32     135      32     821      14      18      14    1417

Cuts at:   3856    3888    3930    3962    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1417     821     135      42      32      32      32
                  32      18      14      14
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2559    2805     669 
   Size:        988     281     621     246    3079

  Fragments arranged by size: 

                3079     988     621     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5215
 
HpaI GTT'AAC

Cuts at:   2805    2805 
   Size:       5215
 
KpnI G_GTAC'C

Cuts at:   2540    2540 
   Size:       5215
 
MfeI C'AATT_G

Cuts at:   2814    2814 
   Size:       5215
 
MluI A'CGCG_T

Cuts at:   5091    5091 
   Size:       5215
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2331    4329    4513    1305 
   Size:       1026    1998     184    2007

  Fragments arranged by size: 

                2007    1998    1026     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3393

  Fragments arranged by size: 

                3393    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2321    2357    3173    3532    3691     518 
   Size:        648    1155      36     816     359     159    2042

  Fragments arranged by size: 

                2042    1155     816     648     359     159      36
 
MspA1I CmG'CkG

Cuts at:   1606    2386    2631    3308    4456    4701    1606 
   Size:        780     245     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     780     677     245     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2124     513 
   Size:        552    1059    3604

  Fragments arranged by size: 

                3604    1059     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5215
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5215
 
NotI GC'GGCC_GC

Cuts at:   2662    2662 
   Size:       5215
 
NspI r_CATG'y

Cuts at:   1132    1229    2146    2573    4118    1132 
   Size:         97     917     427    1545    2229

  Fragments arranged by size: 

                2229    1545     917     427      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5215
 
PciI A'CATG_T

Cuts at:   4114    4114 
   Size:       5215
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5215
 
PmeI GTTT'AAAC

Cuts at:   2519    2519 
   Size:       5215
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3284    3316    4797    4829    3284 
   Size:         32    1481      32    3670

  Fragments arranged by size: 

                3670    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2116    2116 
   Size:       5215
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5215
 
PsiI TTA'TAA

Cuts at:   2785    2785 
   Size:       5215
 
PstI C_TGCA'G

Cuts at:    830    2567     830 
   Size:       1737    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5215
 
PvuII CAG'CTG

Cuts at:   2386    2386 
   Size:       5215
 
SalI G'TCGA_C

Cuts at:   1936    2557    1936 
   Size:        621    4594
 
SbfI CC_TGCA'GG

Cuts at:   2567    2567 
   Size:       5215
 
ScaI AGT'ACT

Cuts at:   3463    5175    3463 
   Size:       1712    3503
 
SfcI C'TryA_G

Cuts at:    826    1045    2563    3698    4379    4570     826 
   Size:        219    1518    1135     681     191    1471

  Fragments arranged by size: 

                1518    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5215
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5215
 
SmaI CCC'GGG

Cuts at:   1872    2542    1872 
   Size:        670    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3319    4220    4482     820 
   Size:        197     926    1376     901     262    1553

  Fragments arranged by size: 

                1553    1376     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3006     493 
   Size:       2513    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5215
 
SphI G_CATG'C

Cuts at:   1132    2573    1132 
   Size:       1441    3774
 
SspI AAT'ATT

Cuts at:      5      52    3139       5 
   Size:         47    3087    2081

  Fragments arranged by size: 

                3087    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5215
 
StyI C'CwwG_G

Cuts at:    513    1065    2124    2236    2654     513 
   Size:        552    1059     112     418    3074

  Fragments arranged by size: 

                3074    1059     552     418     112
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3242    3259    3412    3597    4042     325 
   Size:       1255    1662      17     153     185     445    1498

  Fragments arranged by size: 

                1662    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3461    5173      96 
   Size:        274      80      33      51    1443    1484    1712     138

  Fragments arranged by size: 

                1712    1484    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5175    5207    5175 
   Size:         32    5183
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5215
 
XbaI T'CTAG_A

Cuts at:   2551    2551 
   Size:       5215
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5215
 
XmaI C'CCGG_G

Cuts at:   1870    2540    1870 
   Size:        670    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2020    2366    3344    2020 
   Size:        346     978    3891

  Fragments arranged by size: 

                3891     978     346
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2409    3023     276 
   Size:         53      83     186     936     875     614    2468

  Fragments arranged by size: 

                2468     936     875     614     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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