(Circular) (Six-base) MAPSORT of: pFN21AE6140.seq Check: 6316 from: 1 to: 5215 pFN21AE6140 5215 bp With 182 enzymes: SgfI * December 8, 2014 13:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2411 3025 278 Size: 53 83 186 936 875 614 2468 Fragments arranged by size: 2468 936 875 614 186 83 53 AccI GT'mk_AC Cuts at: 1937 2558 1937 Size: 621 4594 Acc65I G'GTAC_C Cuts at: 2536 2536 Size: 5215 AclI AA'CG_TT Cuts at: 1563 3342 3715 1563 Size: 1779 373 3063 Fragments arranged by size: 3063 1779 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2304 3278 4662 4930 1632 Size: 672 974 1384 268 1917 Fragments arranged by size: 1917 1384 974 672 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5018 AflIII A'CryG_T Cuts at: 1203 2479 4114 5091 1203 Size: 1276 1635 977 1327 Fragments arranged by size: 1635 1327 1276 977 AgeI A'CCGG_T Cuts at: 4046 4046 Size: 5215 AhdI GACnn_n'nnGTC Cuts at: 2407 3944 2407 Size: 1537 3678 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5215 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2186 1184 Size: 1002 4213 AlwNI CAG_nnn'CTG Cuts at: 2958 4530 2958 Size: 1572 3643 ApaLI G'TGCA_C Cuts at: 3272 4428 3272 Size: 1156 4059 ApoI r'AATT_y Cuts at: 1493 2524 2750 2902 5011 1493 Size: 1031 226 152 2109 1697 Fragments arranged by size: 2109 1697 1031 226 152 AseI AT'TA_AT Cuts at: 160 1032 3769 160 Size: 872 2737 1606 Fragments arranged by size: 2737 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5215 AvaI C'yCGr_G Cuts at: 1870 1943 2540 1870 Size: 73 597 4545 Fragments arranged by size: 4545 597 73 AvrII C'CTAG_G Cuts at: 2236 2236 Size: 5215 BamHI G'GATC_C Cuts at: 2545 2912 2545 Size: 367 4848 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2351 2536 3991 618 Size: 325 217 627 564 185 1455 1842 Fragments arranged by size: 1842 1455 627 564 325 217 185 BanII G_rGCy'C Cuts at: 1391 2190 2534 1391 Size: 799 344 4072 Fragments arranged by size: 4072 799 344 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 928 Size: 971 393 3851 Fragments arranged by size: 3851 971 393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3406 3440 1903 Size: 34 1469 34 3678 Fragments arranged by size: 3678 1469 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3107 4317 3107 Size: 1210 4005 BclI T'GATC_A Cuts at: 1548 1548 Size: 5215 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2108 3826 136 Size: 107 122 71 631 605 436 1718 1525 Fragments arranged by size: 1718 1525 631 605 436 122 107 71 BglII A'GATC_T Cuts at: 5210 5210 Size: 5215 BlpI GC'TnA_GC Cuts at: 2632 2632 Size: 5215 Bme1580I G_kGCm'C Cuts at: 1790 2104 2458 3276 4432 1790 Size: 314 354 818 1156 2573 Fragments arranged by size: 2573 1156 818 354 314 BmgBI CAC'GTC Cuts at: 1318 2266 2398 2482 1318 Size: 948 132 84 4051 Fragments arranged by size: 4051 948 132 84 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2946 3904 442 Size: 416 53 83 399 24 1529 958 1753 Fragments arranged by size: 1753 1529 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5215 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3875 1239 Size: 2636 2579 Bpu10I CC'TnA_GC Cuts at: 2138 2138 Size: 5215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3340 4205 4503 3340 Size: 865 298 4052 Fragments arranged by size: 4052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3878 882 Size: 617 2379 2219 Fragments arranged by size: 2379 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3006 493 Size: 711 1802 2702 Fragments arranged by size: 2702 1802 711 BsaBI GATnn'nnATC Cuts at: 2510 5209 2510 Size: 2699 2516 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2244 2408 2461 3022 Size: 53 83 186 936 711 164 53 561 Cuts at: 3022 3404 4039 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 711 635 561 382 186 164 83 53 53 BsaWI w'CCGG_w Cuts at: 2496 3648 4046 4320 4467 4878 2496 Size: 1152 398 274 147 411 2833 Fragments arranged by size: 2833 1152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4990 Fragments arranged by size: 4990 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 2062 2074 2077 1186 Size: 873 3 12 3 4324 Fragments arranged by size: 4324 873 12 3 3 BseYI C'CCAG_C Cuts at: 2200 4418 4914 2200 Size: 2218 496 2501 Fragments arranged by size: 2501 2218 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4642 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2665 3426 4454 1540 Size: 275 186 664 761 1028 2301 Fragments arranged by size: 2301 1028 761 664 275 186 BsiHKAI G_wGCw'C Cuts at: 3276 3361 4432 3276 Size: 85 1071 4059 Fragments arranged by size: 4059 1071 85 BsmI GAATG_Cn' Cuts at: 2726 2819 2726 Size: 93 5122 BsmBI CGTCTCn'nnnn_ Cuts at: 2235 2235 Size: 5215 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2190 2458 2534 3276 3361 4432 Size: 399 314 86 268 76 742 85 1071 Cuts at: 4432 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 314 268 86 85 76 BspEI T'CCGG_A Cuts at: 2496 4878 2496 Size: 2382 2833 BspHI T'CATG_A Cuts at: 2358 3104 4834 2358 Size: 746 1730 2739 Fragments arranged by size: 2739 1730 746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2570 844 Size: 1726 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2036 3102 1394 Size: 429 213 1066 3507 Fragments arranged by size: 3507 1066 429 213 BsrDI GCAATG_nn' Cuts at: 65 1248 3710 3884 65 Size: 1183 2462 174 1396 Fragments arranged by size: 2462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3859 4046 1700 Size: 110 2049 187 2869 Fragments arranged by size: 2869 2049 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5215 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5215 BssSI C'ACGA_G Cuts at: 3275 4287 3275 Size: 1012 4203 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5215 BstBI TT'CG_AA Cuts at: 4019 4019 Size: 5215 BstEII G'GTnAC_C Cuts at: 1184 2413 1184 Size: 1229 3986 BstYI r'GATC_y Cuts at: 1972 2545 2912 3297 3314 4766 4852 4864 5210 Size: 573 367 385 17 1452 86 12 346 Cuts at: 5210 1972 Size: 1977 Fragments arranged by size: 1977 1452 573 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2124 513 Size: 552 1059 3604 Fragments arranged by size: 3604 1059 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4476 Fragments arranged by size: 4476 683 56 BtsI GCAGTG_nn' Cuts at: 2731 3525 3545 2731 Size: 794 20 4401 Fragments arranged by size: 4401 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5215 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5180 DraI TTT'AAA Cuts at: 2519 2519 Size: 5215 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2294 2354 4222 809 Size: 507 978 60 1868 1802 Fragments arranged by size: 1868 1802 978 507 60 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2662 3551 5180 Size: 54 1425 50 275 18 832 889 1629 Cuts at: 5180 8 Size: 43 Fragments arranged by size: 1629 1425 889 832 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2662 1537 Size: 275 850 4090 Fragments arranged by size: 4090 850 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2026 2062 2080 3145 1353 Size: 673 36 18 1065 3423 Fragments arranged by size: 3423 1065 673 36 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3802 4176 4322 387 Size: 1064 138 2213 374 146 1280 Fragments arranged by size: 2213 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2304 3278 3875 4662 4930 1239 Size: 393 672 974 597 787 268 1524 Fragments arranged by size: 1524 974 787 672 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2116 2134 2116 Size: 18 5197 EcoRI G'AATT_C Cuts at: 2524 2902 2524 Size: 378 4837 FspI TGC'GCA Cuts at: 3721 3721 Size: 5215 HaeII r_GCGC'y Cuts at: 1678 4362 1678 Size: 2684 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2393 2407 2425 2439 3856 Size: 32 135 32 821 14 18 14 1417 Cuts at: 3856 3888 3930 3962 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 821 135 42 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2559 2805 669 Size: 988 281 621 246 3079 Fragments arranged by size: 3079 988 621 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5215 HpaI GTT'AAC Cuts at: 2805 2805 Size: 5215 KpnI G_GTAC'C Cuts at: 2540 2540 Size: 5215 MfeI C'AATT_G Cuts at: 2814 2814 Size: 5215 MluI A'CGCG_T Cuts at: 5091 5091 Size: 5215 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 4329 4513 1305 Size: 1026 1998 184 2007 Fragments arranged by size: 2007 1998 1026 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3393 Fragments arranged by size: 3393 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 2357 3173 3532 3691 518 Size: 648 1155 36 816 359 159 2042 Fragments arranged by size: 2042 1155 816 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2386 2631 3308 4456 4701 1606 Size: 780 245 677 1148 245 2120 Fragments arranged by size: 2120 1148 780 677 245 245 NcoI C'CATG_G Cuts at: 513 1065 2124 513 Size: 552 1059 3604 Fragments arranged by size: 3604 1059 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5215 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5215 NotI GC'GGCC_GC Cuts at: 2662 2662 Size: 5215 NspI r_CATG'y Cuts at: 1132 1229 2146 2573 4118 1132 Size: 97 917 427 1545 2229 Fragments arranged by size: 2229 1545 917 427 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5215 PciI A'CATG_T Cuts at: 4114 4114 Size: 5215 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5215 PmeI GTTT'AAAC Cuts at: 2519 2519 Size: 5215 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3284 3316 4797 4829 3284 Size: 32 1481 32 3670 Fragments arranged by size: 3670 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2116 2116 Size: 5215 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5215 PsiI TTA'TAA Cuts at: 2785 2785 Size: 5215 PstI C_TGCA'G Cuts at: 830 2567 830 Size: 1737 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5215 PvuII CAG'CTG Cuts at: 2386 2386 Size: 5215 SalI G'TCGA_C Cuts at: 1936 2557 1936 Size: 621 4594 SbfI CC_TGCA'GG Cuts at: 2567 2567 Size: 5215 ScaI AGT'ACT Cuts at: 3463 5175 3463 Size: 1712 3503 SfcI C'TryA_G Cuts at: 826 1045 2563 3698 4379 4570 826 Size: 219 1518 1135 681 191 1471 Fragments arranged by size: 1518 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5215 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5215 SmaI CCC'GGG Cuts at: 1872 2542 1872 Size: 670 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3319 4220 4482 820 Size: 197 926 1376 901 262 1553 Fragments arranged by size: 1553 1376 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3006 493 Size: 2513 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5215 SphI G_CATG'C Cuts at: 1132 2573 1132 Size: 1441 3774 SspI AAT'ATT Cuts at: 5 52 3139 5 Size: 47 3087 2081 Fragments arranged by size: 3087 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5215 StyI C'CwwG_G Cuts at: 513 1065 2124 2236 2654 513 Size: 552 1059 112 418 3074 Fragments arranged by size: 3074 1059 552 418 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3242 3259 3412 3597 4042 325 Size: 1255 1662 17 153 185 445 1498 Fragments arranged by size: 1662 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3461 5173 96 Size: 274 80 33 51 1443 1484 1712 138 Fragments arranged by size: 1712 1484 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5175 5207 5175 Size: 32 5183 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5215 XbaI T'CTAG_A Cuts at: 2551 2551 Size: 5215 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5215 XmaI C'CCGG_G Cuts at: 1870 2540 1870 Size: 670 4545 XmnI GAAnn'nnTTC Cuts at: 2020 2366 3344 2020 Size: 346 978 3891 Fragments arranged by size: 3891 978 346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2409 3023 276 Size: 53 83 186 936 875 614 2468 Fragments arranged by size: 2468 936 875 614 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI