################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AhdI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BseAI BsiWI BsmFI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HindIII HpaI KpnI MaeII MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tru9I Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AcsI 585 GAATTT [GA]^AATT[CT] AcyI 227 GGCGCC G[GA]^CG[CT]C AgeI 284 ACCGGT A^CCGGT Alw44I 337 GTGCAC G^TGCAC AlwNI 76 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 141 CCTGAGG CC^T[ACGT]AGG Aor51HI 243 AGCGCT AGC^GCT ApaI 15 GGGCCC GGGCC^C ApaLI 337 GTGCAC G^TGCAC ApoI 585 GAATTT [GA]^AATT[CT] BalI 368 TGGCCA TGG^CCA BanI 227 GGCGCC G^G[CT][GA]CC BbeI 227 GGCGCC GGCGC^C BbsI 393 GAAGAC GAAGAC(2/6) BbvCI 316 GCTGAGG CCTCAGC(-5/-2) BlpI 36 GCTCAGC GC^T[ACGT]AGC BpmI 433 CTCCAG CTGGAG(16/14) Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC BpuAI 393 GAAGAC GAAGAC(2/6) BsaHI 227 GGCGCC G[GA]^CG[CT]C BsaWI 284 ACCGGT [AT]^CCGG[AT] BseRI 184 GAGGAG GAGGAG(10/8) BsmAI 259 GAGAC GTCTC(1/5) BsmBI 259 GAGACG CGTCTC(1/5) BspHI 389 TCATGA T^CATGA BspMI 942 ACCTGC ACCTGC(4/8) BsrDI 499 GCAATG GCAATG(2/0) BsrFI 284 ACCGGT [GA]^CCGG[CT] BsrI 902 ACTGG ACTGG(1/-1) BstBI 686 TTCGAA TT^CGAA BstYI 219 GGATCT [GA]^GATC[CT] Bsu36I 141 CCTGAGG CC^T[ACGT]AGG CelII 36 GCTCAGC GC^T[ACGT]AGC Cfr10I 284 ACCGGT [GA]^CCGG[CT] DraII 204 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 116 CCGCGG C^C[GA][CT]GG EarI 232 CTCTTC CTCTTC(1/4) Eco47III 243 AGCGCT AGC^GCT Eco81I 141 CCTGAGG CC^T[ACGT]AGG EcoO109I 204 GGGGCCT [GA]G^G[ACGT]CC[CT] Hin1I 227 GGCGCC G[GA]^CG[CT]C HincII 610 GTTGAC GT[CT]^[GA]AC HindII 610 GTTGAC GT[CT]^[GA]AC KasI 227 GGCGCC G^GCGCC Ksp632I 232 CTCTTC CTCTTC(1/4) KspI 116 CCGCGG CCGC^GG MaeIII 559 GTTAC ^GT[ACGT]AC MflI 219 GGATCT [GA]^GATC[CT] MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA NarI 227 GGCGCC GG^CGCC NspV 686 TTCGAA TT^CGAA PflMI 495 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 284 ACCGGT A^CCGGT PvuII 63 CAGCTG CAG^CTG RcaI 389 TCATGA T^CATGA SacI 34 GAGCTC GAGCT^C SacII 116 CCGCGG CCGC^GG SfcI 728 CTATAG C^T[GA][CT]AG SfoI 227 GGCGCC GGC^GCC SfuI 686 TTCGAA TT^CGAA SmlI 593 CTCAAG C^T[CT][GA]AG SspI 984 AATATT AAT^ATT Van91I 495 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 219 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 794 GTAC GT^AC 817 GTAC AvaI 18 CCCGAG C^[CT]CG[GA]G 256 CCCGAG BbvI 90 GCAGC GCAGC(8/12) 29 GCTGC BciVI 516 GGATAC GTATCC(6/5) 570 GTATCC BcnI 13 CCGGG CC^[GC]GG 71 CCGGG BfaI 524 CTAG C^TAG 695 CTAG BsaJI 116 CCGCGG C^C[ACGT][ACGT]GG 208 CCTGGG BsgI 88 GTGCAG GTGCAG(16/14) 669 GTGCAG BsiEI 119 CGGCCG CG[GA][CT]^CG 161 CGGCCG BsoBI 18 CCCGAG C^[CT]CG[GA]G 256 CCCGAG Bsp1407I 793 TGTACA T^GTACA 816 TGTACA BsrGI 793 TGTACA T^GTACA 816 TGTACA EagI 119 CGGCCG C^GGCCG 161 CGGCCG EclXI 119 CGGCCG C^GGCCG 161 CGGCCG Eco52I 119 CGGCCG C^GGCCG 161 CGGCCG MaeI 524 CTAG C^TAG 695 CTAG MslI 159 CACGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 340 CACTGTCATG MspA1I 63 CAGCTG C[AC]G^C[GT]G 116 CCGCGG NciI 13 CCGGG CC^[GC]GG 71 CCGGG RsaI 794 GTAC GT^AC 817 GTAC SfaNI 78 GATGC GCATC(5/9) 155 GCATC SspBI 793 TGTACA T^GTACA 816 TGTACA TfiI 438 GATTC G^A[AT]TC 829 GAATC TseI 29 GCTGC G^C[AT]GC 90 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaII BanII BmyI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI Tsp509I TspRI TthHB8I