Restriction map for pFN21AE5403
 (Circular) (Six-base) MAPSORT of: pFN21AE5403.seq  Check: 2271  from: 1  to: 5725



pFN21AE5403  5725 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2956    2956 
   Size:       5725
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3535     278 
   Size:         53      83     186     936    1999    2468

  Fragments arranged by size: 

                2468    1999     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3068    1937 
   Size:       1131    4594
 
Acc65I G'GTAC_C

Cuts at:   3046    3046 
   Size:       5725
 
AclI AA'CG_TT

Cuts at:   1563    3852    4225    1563 
   Size:       2289     373    3063

  Fragments arranged by size: 

                3063    2289     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3788    5172    5440    1632 
   Size:       2156    1384     268    1917

  Fragments arranged by size: 

                2156    1917    1384     268
 
AfeI AGC'GCT

Cuts at:   2250    2250 
   Size:       5725
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5528
 
AflIII A'CryG_T

Cuts at:   1203    4624    5601    1203 
   Size:       3421     977    1327

  Fragments arranged by size: 

                3421    1327     977
 
AgeI A'CCGG_T

Cuts at:   2289    4556    2289 
   Size:       2267    3458
 
AhdI GACnn_n'nnGTC

Cuts at:   4454    4454 
   Size:       5725
 
AleI CACnn'nnGTG

Cuts at:   1166    2168    1166 
   Size:       1002    4723
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2367    1184 
   Size:       1183    4542
 
AlwNI CAG_nnn'CTG

Cuts at:   2086    3468    5040    2086 
   Size:       1382    1572    2771

  Fragments arranged by size: 

                2771    1572    1382
 
ApaI G_GGCC'C

Cuts at:   2024    2024 
   Size:       5725
 
ApaLI G'TGCA_C

Cuts at:   2342    3782    4938    2342 
   Size:       1440    1156    3129

  Fragments arranged by size: 

                3129    1440    1156
 
ApoI r'AATT_y

Cuts at:   1493    2590    3034    3260    3412    5521    1493 
   Size:       1097     444     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1097     444     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4279     160 
   Size:        872    3247    1606

  Fragments arranged by size: 

                3247    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5725
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2023    2261    3050    1870 
   Size:         73      80     238     789    4545

  Fragments arranged by size: 

                4545     789     238      80      73
 
BamHI G'GATC_C

Cuts at:   3055    3422    3055 
   Size:        367    5358
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2232    3046    4501     618 
   Size:        325     217     627     445     814    1455    1842

  Fragments arranged by size: 

                1842    1455     814     627     445     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2024    2043    2196    3044    1391 
   Size:        633      19     153     848    4072

  Fragments arranged by size: 

                4072     848     633     153      19
 
BbeI G_GCGC'C

Cuts at:   2236    2236 
   Size:       5725
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2405     928 
   Size:        971     506    4248

  Fragments arranged by size: 

                4248     971     506
 
BbvCI CC'TCA_GC

Cuts at:   2322    2322 
   Size:       5725
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3916    3950    1903 
   Size:         34    1979      34    3678

  Fragments arranged by size: 

                3678    1979      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2515    2586    3617    4827    2515 
   Size:         71    1031    1210    3413

  Fragments arranged by size: 

                3413    1210    1031      71
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5725
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4336     136 
   Size:        107     122      71     631     605    2664    1525

  Fragments arranged by size: 

                2664    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5720    5720 
   Size:       5725
 
BlpI GC'TnA_GC

Cuts at:   2042    3142    2042 
   Size:       1100    4625
 
Bme1580I G_kGCm'C

Cuts at:   1790    2024    2346    3786    4942    1790 
   Size:        234     322    1440    1156    2573

  Fragments arranged by size: 

                2573    1440    1156     322     234
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5725
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3456    4414     442 
   Size:        416      53      83     399      24    2039     958    1753

  Fragments arranged by size: 

                2039    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5725
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2808    2808 
   Size:       5725
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2423    4385    1239 
   Size:       1184    1962    2579

  Fragments arranged by size: 

                2579    1962    1184
 
Bpu10I CC'TnA_GC

Cuts at:   2322    2322 
   Size:       5725
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2583    3850    4715    5013    2583 
   Size:       1267     865     298    3295

  Fragments arranged by size: 

                3295    1267     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4388     882 
   Size:        617    2889    2219

  Fragments arranged by size: 

                2889    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3516     493 
   Size:        711    2312    2702

  Fragments arranged by size: 

                2702    2312     711
 
BsaBI GATnn'nnATC

Cuts at:   5719    5719 
   Size:       5725
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2233    3532    3914    4549 
   Size:         53      83     186     936     700    1299     382     635

Cuts at:   4549     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1299     936     700     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2289    4158    4556    4830    4977    5388    2289 
   Size:       1869     398     274     147     411    2626

  Fragments arranged by size: 

                2626    1869     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5500

  Fragments arranged by size: 

                5500     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2204    1186 
   Size:       1018    4707
 
BseYI C'CCAG_C

Cuts at:   2051    2195    4928    5424    2051 
   Size:        144    2733     496    2352

  Fragments arranged by size: 

                2733    2352     496     144
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2114    2695    1163 
   Size:        573     378     581    4193

  Fragments arranged by size: 

                4193     581     573     378
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2127    2169    3175    3936    4964    1540 
   Size:        275     186     126      42    1006     761    1028    2301

  Fragments arranged by size: 

                2301    1028    1006     761     275     186     126      42
            
 
BsiHKAI G_wGCw'C

Cuts at:   2043    2346    2927    3786    3871    4942    2043 
   Size:        303     581     859      85    1071    2826

  Fragments arranged by size: 

                2826    1071     859     581     303      85
 
BsmI GAATG_Cn'

Cuts at:   3236    3329    3236 
   Size:         93    5632
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2258    2258 
   Size:       5725
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2024    2043    2196    2346    2927    3044    3786 
   Size:        399     234      19     153     150     581     117     742

Cuts at:   3786    3871    4942    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     581     399     234     153     150
                 117      85      19
 
BspEI T'CCGG_A

Cuts at:   5388    5388 
   Size:       5725
 
BspHI T'CATG_A

Cuts at:   2394    3614    5344    2394 
   Size:       1220    1730    2775

  Fragments arranged by size: 

                2775    1730    1220
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2956    3080     844 
   Size:       2112     124    3489

  Fragments arranged by size: 

                3489    2112     124
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3612    1394 
   Size:        429    1789    3507

  Fragments arranged by size: 

                3507    1789     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2511    4220    4394      65 
   Size:       1183    1263    1709     174    1396

  Fragments arranged by size: 

                1709    1396    1263    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2289    4369    4556    1700 
   Size:        110     479    2080     187    2869

  Fragments arranged by size: 

                2869    2080     479     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2798    2821      96 
   Size:       2702      23    3000

  Fragments arranged by size: 

                3000    2702      23
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5725
 
BssSI C'ACGA_G

Cuts at:   3785    4797    3785 
   Size:       1012    4713
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5725
 
BstBI TT'CG_AA

Cuts at:   2692    4529    2692 
   Size:       1837    3888
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5725
 
BstYI r'GATC_y

Cuts at:   1972    2224    3055    3422    3807    3824    5276    5362    5374 
   Size:        252     831     367     385      17    1452      86      12

Cuts at:   5374    5720    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     831     385     367     346     252      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2147    2147 
   Size:       5725
 
BtgI C'CryG_G

Cuts at:    513    1065    2121     513 
   Size:        552    1056    4117

  Fragments arranged by size: 

                4117    1056     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4986

  Fragments arranged by size: 

                4986     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2890    3241    4035    4055    2890 
   Size:        351     794      20    4560

  Fragments arranged by size: 

                4560     794     351      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5725
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5690
 
DraI TTT'AAA

Cuts at:   3029    3029 
   Size:       5725
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4732     809 
   Size:        507    3416    1802

  Fragments arranged by size: 

                3416    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2124    2166    2373 
   Size:         54    1425      50     275      18     294      42     207

Cuts at:   2373    3172    4061    5690       8 
   Size:        799     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     799     294     275     207      54
                  50      43      42      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2124    2166    3172    1537 
   Size:        275     312      42    1006    4090

  Fragments arranged by size: 

                4090    1006     312     275      42
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2243    3655    1353 
   Size:        890    1412    3423

  Fragments arranged by size: 

                3423    1412     890
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4312    4686    4832     387 
   Size:       1064     138    2723     374     146    1280

  Fragments arranged by size: 

                2723    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2041    2041 
   Size:       5725
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2423    3788    4385    5172    5440    1239 
   Size:        393     791    1365     597     787     268    1524

  Fragments arranged by size: 

                1524    1365     791     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2210    2210 
   Size:       5725
 
EcoRI G'AATT_C

Cuts at:   3034    3412    3034 
   Size:        378    5347
 
FspI TGC'GCA

Cuts at:   4231    4231 
   Size:       5725
 
HaeII r_GCGC'y

Cuts at:   1678    2058    2236    2252    4872    1678 
   Size:        380     178      16    2620    2531

  Fragments arranged by size: 

                2620    2531     380     178      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4366    4398    4440    4472    1373 
   Size:         32     135      32    2794      32      42      32    2626

  Fragments arranged by size: 

                2794    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2617    3069    3315     669 
   Size:        988     281     679     452     246    3079

  Fragments arranged by size: 

                3079     988     679     452     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5725
 
HpaI GTT'AAC

Cuts at:   3315    3315 
   Size:       5725
 
KasI G'GCGC_C

Cuts at:   2232    2232 
   Size:       5725
 
KpnI G_GTAC'C

Cuts at:   3050    3050 
   Size:       5725
 
MfeI C'AATT_G

Cuts at:   3324    3324 
   Size:       5725
 
MluI A'CGCG_T

Cuts at:   5601    5601 
   Size:       5725
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    1989    4839    5023    1305 
   Size:        684    2850     184    2007

  Fragments arranged by size: 

                2850    2007     684     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2375      10 
   Size:         54    1425     343     543    3360

  Fragments arranged by size: 

                3360    1425     543     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2168    2349    3683    4042    4201     518 
   Size:        648    1002     181    1334     359     159    2042

  Fragments arranged by size: 

                2042    1334    1002     648     359     181     159
 
MspA1I CmG'CkG

Cuts at:   1606    2070    2123    3141    3818    4966    5211    1606 
   Size:        464      53    1018     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1018     677     464     245      53
 
NarI GG'CG_CC

Cuts at:   2233    2233 
   Size:       5725
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5173
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5725
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5725
 
NotI GC'GGCC_GC

Cuts at:   3172    3172 
   Size:       5725
 
NspI r_CATG'y

Cuts at:   1132    1229    3083    4628    1132 
   Size:         97    1854    1545    2229

  Fragments arranged by size: 

                2229    1854    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5725
 
PciI A'CATG_T

Cuts at:   4624    4624 
   Size:       5725
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2506    1108 
   Size:       1398    4327
 
PmeI GTTT'AAAC

Cuts at:   3029    3029 
   Size:       5725
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3794    3826    5307    5339    3794 
   Size:         32    1481      32    4180

  Fragments arranged by size: 

                4180    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5725
 
PsiI TTA'TAA

Cuts at:   2541    3295    2541 
   Size:        754    4971
 
PspOMI G'GGCC_C

Cuts at:   2020    2020 
   Size:       5725
 
PstI C_TGCA'G

Cuts at:    830    3077     830 
   Size:       2247    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5725
 
PvuII CAG'CTG

Cuts at:   2070    2070 
   Size:       5725
 
SacI G_AGCT'C

Cuts at:   2043    2043 
   Size:       5725
 
SacII CC_GC'GG

Cuts at:   2124    2124 
   Size:       5725
 
SalI G'TCGA_C

Cuts at:   1936    3067    1936 
   Size:       1131    4594
 
SbfI CC_TGCA'GG

Cuts at:   3077    3077 
   Size:       5725
 
ScaI AGT'ACT

Cuts at:   3973    5685    3973 
   Size:       1712    4013
 
SfcI C'TryA_G

Cuts at:    826    1045    2733    3073    4208    4889    5080     826 
   Size:        219    1688     340    1135     681     191    1471

  Fragments arranged by size: 

                1688    1471    1135     681     340     219     191
 
SfoI GGC'GCC

Cuts at:   2234    2234 
   Size:       5725
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5725
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5725
 
SmaI CCC'GGG

Cuts at:   1872    3052    1872 
   Size:       1180    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2598    3829    4730    4992     820 
   Size:        197     926     655    1231     901     262    1553

  Fragments arranged by size: 

                1553    1231     926     901     655     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3516     493 
   Size:       3023    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5725
 
SphI G_CATG'C

Cuts at:   1132    3083    1132 
   Size:       1951    3774
 
SspI AAT'ATT

Cuts at:      5      52    2991    3649       5 
   Size:         47    2939     658    2081

  Fragments arranged by size: 

                2939    2081     658      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5725
 
StyI C'CwwG_G

Cuts at:    513    1065    3164     513 
   Size:        552    2099    3074

  Fragments arranged by size: 

                3074    2099     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2297    3752    3769    3922    4107    4552     325 
   Size:       1255     717    1455      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1455    1255     717     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2798    2821    3971 
   Size:        274      80      33      51    1443     821      23    1150

Cuts at:   3971    5683      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1150     821     274     138      80      51
                  33      23
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2502    2534    5685    5717    2502 
   Size:         32    3151      32    2510

  Fragments arranged by size: 

                3151    2510      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5725
 
XbaI T'CTAG_A

Cuts at:   3061    3061 
   Size:       5725
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5725
 
XmaI C'CCGG_G

Cuts at:   1870    3050    1870 
   Size:       1180    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3854    3854 
   Size:       5725
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3533     276 
   Size:         53      83     186     936    1999    2468

  Fragments arranged by size: 

                2468    1999     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI
     PvuI    PvuII     SacI    SacII     SalI     SbfI     ScaI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BaeI    BfrBI    BsiWI    BstXI  BstZ17I
   DraIII    EcoNI    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI
    RsrII    SanDI     SapI    SexAI     SfiI     SrfI     SwaI     XcmI
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