(Circular) (Six-base) MAPSORT of: pFN21AE5403.seq Check: 2271 from: 1 to: 5725 pFN21AE5403 5725 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2956 2956 Size: 5725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AfeI AGC'GCT Cuts at: 2250 2250 Size: 5725 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 2289 4556 2289 Size: 2267 3458 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 2168 1166 Size: 1002 4723 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2367 1184 Size: 1183 4542 AlwNI CAG_nnn'CTG Cuts at: 2086 3468 5040 2086 Size: 1382 1572 2771 Fragments arranged by size: 2771 1572 1382 ApaI G_GGCC'C Cuts at: 2024 2024 Size: 5725 ApaLI G'TGCA_C Cuts at: 2342 3782 4938 2342 Size: 1440 1156 3129 Fragments arranged by size: 3129 1440 1156 ApoI r'AATT_y Cuts at: 1493 2590 3034 3260 3412 5521 1493 Size: 1097 444 226 152 2109 1697 Fragments arranged by size: 2109 1697 1097 444 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2261 3050 1870 Size: 73 80 238 789 4545 Fragments arranged by size: 4545 789 238 80 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 3046 4501 618 Size: 325 217 627 445 814 1455 1842 Fragments arranged by size: 1842 1455 814 627 445 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2043 2196 3044 1391 Size: 633 19 153 848 4072 Fragments arranged by size: 4072 848 633 153 19 BbeI G_GCGC'C Cuts at: 2236 2236 Size: 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2405 928 Size: 971 506 4248 Fragments arranged by size: 4248 971 506 BbvCI CC'TCA_GC Cuts at: 2322 2322 Size: 5725 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2515 2586 3617 4827 2515 Size: 71 1031 1210 3413 Fragments arranged by size: 3413 1210 1031 71 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 2042 3142 2042 Size: 1100 4625 Bme1580I G_kGCm'C Cuts at: 1790 2024 2346 3786 4942 1790 Size: 234 322 1440 1156 2573 Fragments arranged by size: 2573 1440 1156 322 234 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3456 4414 442 Size: 416 53 83 399 24 2039 958 1753 Fragments arranged by size: 2039 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2808 2808 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 4385 1239 Size: 1184 1962 2579 Fragments arranged by size: 2579 1962 1184 Bpu10I CC'TnA_GC Cuts at: 2322 2322 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2583 3850 4715 5013 2583 Size: 1267 865 298 3295 Fragments arranged by size: 3295 1267 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2233 3532 3914 4549 Size: 53 83 186 936 700 1299 382 635 Cuts at: 4549 275 Size: 1451 Fragments arranged by size: 1451 1299 936 700 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2289 4158 4556 4830 4977 5388 2289 Size: 1869 398 274 147 411 2626 Fragments arranged by size: 2626 1869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5500 Fragments arranged by size: 5500 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 1186 Size: 1018 4707 BseYI C'CCAG_C Cuts at: 2051 2195 4928 5424 2051 Size: 144 2733 496 2352 Fragments arranged by size: 2733 2352 496 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 2695 1163 Size: 573 378 581 4193 Fragments arranged by size: 4193 581 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2127 2169 3175 3936 4964 1540 Size: 275 186 126 42 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 126 42 BsiHKAI G_wGCw'C Cuts at: 2043 2346 2927 3786 3871 4942 2043 Size: 303 581 859 85 1071 2826 Fragments arranged by size: 2826 1071 859 581 303 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 BsmBI CGTCTCn'nnnn_ Cuts at: 2258 2258 Size: 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2043 2196 2346 2927 3044 3786 Size: 399 234 19 153 150 581 117 742 Cuts at: 3786 3871 4942 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 581 399 234 153 150 117 85 19 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 2394 3614 5344 2394 Size: 1220 1730 2775 Fragments arranged by size: 2775 1730 1220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2956 3080 844 Size: 2112 124 3489 Fragments arranged by size: 3489 2112 124 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2511 4220 4394 65 Size: 1183 1263 1709 174 1396 Fragments arranged by size: 1709 1396 1263 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2289 4369 4556 1700 Size: 110 479 2080 187 2869 Fragments arranged by size: 2869 2080 479 187 110 BsrGI T'GTAC_A Cuts at: 96 2798 2821 96 Size: 2702 23 3000 Fragments arranged by size: 3000 2702 23 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 2692 4529 2692 Size: 1837 3888 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2224 3055 3422 3807 3824 5276 5362 5374 Size: 252 831 367 385 17 1452 86 12 Cuts at: 5374 5720 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 831 385 367 346 252 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2147 2147 Size: 5725 BtgI C'CryG_G Cuts at: 513 1065 2121 513 Size: 552 1056 4117 Fragments arranged by size: 4117 1056 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3241 4035 4055 2890 Size: 351 794 20 4560 Fragments arranged by size: 4560 794 351 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2166 2373 Size: 54 1425 50 275 18 294 42 207 Cuts at: 2373 3172 4061 5690 8 Size: 799 889 1629 43 Fragments arranged by size: 1629 1425 889 799 294 275 207 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2124 2166 3172 1537 Size: 275 312 42 1006 4090 Fragments arranged by size: 4090 1006 312 275 42 EarI CTCTTCn'nnn_ Cuts at: 1353 2243 3655 1353 Size: 890 1412 3423 Fragments arranged by size: 3423 1412 890 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2041 2041 Size: 5725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 3788 4385 5172 5440 1239 Size: 393 791 1365 597 787 268 1524 Fragments arranged by size: 1524 1365 791 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2210 2210 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2058 2236 2252 4872 1678 Size: 380 178 16 2620 2531 Fragments arranged by size: 2620 2531 380 178 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373 Size: 32 135 32 2794 32 42 32 2626 Fragments arranged by size: 2794 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2617 3069 3315 669 Size: 988 281 679 452 246 3079 Fragments arranged by size: 3079 988 679 452 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KasI G'GCGC_C Cuts at: 2232 2232 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 4839 5023 1305 Size: 684 2850 184 2007 Fragments arranged by size: 2850 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3360 Fragments arranged by size: 3360 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2349 3683 4042 4201 518 Size: 648 1002 181 1334 359 159 2042 Fragments arranged by size: 2042 1334 1002 648 359 181 159 MspA1I CmG'CkG Cuts at: 1606 2070 2123 3141 3818 4966 5211 1606 Size: 464 53 1018 677 1148 245 2120 Fragments arranged by size: 2120 1148 1018 677 464 245 53 NarI GG'CG_CC Cuts at: 2233 2233 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 3083 4628 1132 Size: 97 1854 1545 2229 Fragments arranged by size: 2229 1854 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2506 1108 Size: 1398 4327 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 2541 3295 2541 Size: 754 4971 PspOMI G'GGCC_C Cuts at: 2020 2020 Size: 5725 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2070 2070 Size: 5725 SacI G_AGCT'C Cuts at: 2043 2043 Size: 5725 SacII CC_GC'GG Cuts at: 2124 2124 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2733 3073 4208 4889 5080 826 Size: 219 1688 340 1135 681 191 1471 Fragments arranged by size: 1688 1471 1135 681 340 219 191 SfoI GGC'GCC Cuts at: 2234 2234 Size: 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2598 3829 4730 4992 820 Size: 197 926 655 1231 901 262 1553 Fragments arranged by size: 1553 1231 926 901 655 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 2991 3649 5 Size: 47 2939 658 2081 Fragments arranged by size: 2939 2081 658 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 3164 513 Size: 552 2099 3074 Fragments arranged by size: 3074 2099 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 3752 3769 3922 4107 4552 325 Size: 1255 717 1455 17 153 185 445 1498 Fragments arranged by size: 1498 1455 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2798 2821 3971 Size: 274 80 33 51 1443 821 23 1150 Cuts at: 3971 5683 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1150 821 274 138 80 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 5685 5717 2502 Size: 32 3151 32 2510 Fragments arranged by size: 3151 2510 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI