################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HgaI HindIII HpaI HphI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 582 GACGTC GACGT^C AcyI 582 GACGTC G[GA]^CG[CT]C AlwNI 135 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 463 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 75 GGATCC G^GATCC BcgI 587 CGACTGCGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 499 CCGGG CC^[GC]GG BsaHI 582 GACGTC G[GA]^CG[CT]C BsaI 687 GAGACC GGTCTC(1/5) BsgI 261 CTGCAC GTGCAG(16/14) BsiYI 492 CCTCTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 492 CCTCTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 687 GAGAC GTCTC(1/5) BspMI 166 GCAGGT ACCTGC(4/8) BsrI 691 CCAGT ACTGG(1/-1) BstYI 75 GGATCC [GA]^GATC[CT] DraII 178 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 598 CTCTTC CTCTTC(1/4) EcoO109I 178 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 519 CCCGC CCCGC(4/6) HapII 499 CCGG C^CGG Hin1I 582 GACGTC G[GA]^CG[CT]C HinfI 123 GAATC G^A[ACGT]TC HpaII 499 CCGG C^CGG Ksp632I 598 CTCTTC CTCTTC(1/4) MflI 75 GGATCC [GA]^GATC[CT] MspI 499 CCGG C^CGG NciI 499 CCGGG CC^[GC]GG NdeI 189 CATATG CA^TATG NlaIII 300 CATG CATG^ NlaIV 75 GGATCC GG[ACGT]^[ACGT]CC NspI 299 GCATGT [GA]CATG^[CT] PflFI 463 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 178 AGGACCT [GA]G^G[AT]CC[CT] SapI 597 GCTCTTC GCTCTTC(1/4) SfcI 720 CTGTAG C^T[GA][CT]AG SmlI 704 CTCAAG C^T[CT][GA]AG TfiI 123 GAATC G^A[AT]TC Tth111I 463 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 29 CCACTCTGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 75 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 398 GTCGAC GT^[AC][GT]AC 585 GTCGAC AciI 520 CCGC CCGC(-3/-1) 553 CCGC AfaI 101 GTAC GT^AC 694 GTAC AlwI 76 GATCC GGATC(4/5) 75 GGATC BbvI 296 GCAGC GCAGC(8/12) 725 GCTGC BsrDI 68 CATTGC GCAATG(2/0) 245 GCAATG Cac8I 194 GCAGGC GC[ACGT]^[ACGT]GC 522 GCCTGC CfoI 164 GCGC GCG^C 577 GCGC Fnu4HI 296 GCAGC GC^[ACGT]GC 725 GCTGC HhaI 164 GCGC GCG^C 577 GCGC HinP1I 164 GCGC G^CGC 577 GCGC HincII 398 GTCGAC GT[CT]^[GA]AC 585 GTCGAC HindII 398 GTCGAC GT[CT]^[GA]AC 585 GTCGAC ItaI 296 GCAGC GC^[ACGT]GC 725 GCTGC MseI 374 TTAA T^TAA 638 TTAA MspA1I 9 CAGCTG C[AC]G^C[GT]G 95 CAGCTG MwoI 597 GCTCTTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 719 GCTGTAGCTGC PvuII 9 CAGCTG CAG^CTG 95 CAGCTG RsaI 101 GTAC GT^AC 694 GTAC SalI 398 GTCGAC G^TCGAC 585 GTCGAC TaqI 399 TCGA T^CGA 586 TCGA Tru9I 374 TTAA T^TAA 638 TTAA TseI 296 GCAGC G^C[AT]GC 725 GCTGC TspRI 199 CAGTG CA[GC]TG[ACGT][ACGT]^ 277 CACTG TthHB8I 399 TCGA T^CGA 586 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI AvaII BpmI BsaJI BsmFI BssKI BstF5I BstNI Cfr13I DpnII EcoRII FokI MaeII MaeIII MboI MboII MnlI MvaI NdeII Sau3AI Sau96I ScrFI SfaNI Tsp45I