(Linear) (Six-base) MAPSORT of: KIEE3664.seq Check: 1857 from: 1 to: 1962 KIEE3664.orf With 182 enzymes: SgfI * March 22, 2012 16:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1543 1562 1863 1962 Size: 1543 19 301 99 Fragments arranged by size: 1543 301 99 19 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 269 647 1032 1761 1962 Size: 269 378 385 729 201 Fragments arranged by size: 729 385 378 269 201 AfeI AGC'GCT Cuts at: 0 454 1045 1962 Size: 454 591 917 Fragments arranged by size: 917 591 454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1594 1962 Size: 1594 368 AlwNI CAG_nnn'CTG Cuts at: 0 54 219 234 696 1962 Size: 54 165 15 462 1266 Fragments arranged by size: 1266 462 165 54 15 ApaI G_GGCC'C Cuts at: 0 381 1351 1470 1962 Size: 381 970 119 492 Fragments arranged by size: 970 492 381 119 ApaLI G'TGCA_C Cuts at: 0 1696 1962 Size: 1696 266 AvaI C'yCGr_G Cuts at: 0 179 260 362 499 677 732 831 972 Size: 179 81 102 137 178 55 99 141 Cuts at: 972 1517 1947 1962 Size: 545 430 15 Fragments arranged by size: 545 430 179 178 141 137 102 99 81 55 15 AvrII C'CTAG_G Cuts at: 0 79 1644 1962 Size: 79 1565 318 Fragments arranged by size: 1565 318 79 BanI G'GyrC_C Cuts at: 0 1570 1962 Size: 1570 392 BanII G_rGCy'C Cuts at: 0 381 770 979 1351 1470 1496 1961 1962 Size: 381 389 209 372 119 26 465 1 Fragments arranged by size: 465 389 381 372 209 119 26 1 BbsI GAAGACnn'nnnn_ Cuts at: 0 1406 1962 Size: 1406 556 BglI GCCn_nnn'nGGC Cuts at: 0 1646 1962 Size: 1646 316 BlpI GC'TnA_GC Cuts at: 0 697 1962 Size: 697 1265 Bme1580I G_kGCm'C Cuts at: 0 381 1068 1084 1351 1470 1573 1700 1962 Size: 381 687 16 267 119 103 127 262 Fragments arranged by size: 687 381 267 262 127 119 103 16 BmrI ACTGGGnnnn_n' Cuts at: 0 795 1087 1377 1599 1940 1962 Size: 795 292 290 222 341 22 Fragments arranged by size: 795 341 292 290 222 22 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 183 1962 Size: 183 1779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1621 1962 Size: 1621 341 Bpu10I CC'TnA_GC Cuts at: 0 101 1962 Size: 101 1861 BsaI GGTCTCn'nnnn_ Cuts at: 0 294 735 1962 Size: 294 441 1227 Fragments arranged by size: 1227 441 294 BsaAI yAC'GTr Cuts at: 0 609 1962 Size: 609 1353 BsaHI Gr'CG_yC Cuts at: 0 1122 1962 Size: 1122 840 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 940 970 1920 1950 1962 Size: 940 30 950 30 12 Fragments arranged by size: 950 940 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 139 1962 Size: 37 102 1823 Fragments arranged by size: 1823 102 37 BseYI C'CCAG_C Cuts at: 0 65 174 449 455 631 1089 1218 1283 Size: 65 109 275 6 176 458 129 65 Cuts at: 1283 1360 1378 1566 1636 1706 1797 1962 Size: 77 18 188 70 70 91 165 Fragments arranged by size: 458 275 188 176 165 129 109 91 77 70 70 65 65 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 285 1090 1962 Size: 205 80 805 872 Fragments arranged by size: 872 805 205 80 BsiEI CG_ry'CG Cuts at: 0 538 1962 Size: 538 1424 BsiHKAI G_wGCw'C Cuts at: 0 88 1619 1700 1856 1962 Size: 88 1531 81 156 106 Fragments arranged by size: 1531 156 106 88 81 BsmBI CGTCTCn'nnnn_ Cuts at: 0 731 1461 1962 Size: 731 730 501 Fragments arranged by size: 731 730 501 Bsp1286I G_dGCh'C Cuts at: 0 88 381 770 979 1068 1084 1351 1470 Size: 88 293 389 209 89 16 267 119 Cuts at: 1470 1496 1573 1619 1700 1856 1961 1962 Size: 26 77 46 81 156 105 1 Fragments arranged by size: 389 293 267 209 156 119 105 89 88 81 77 46 26 16 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 533 959 1161 1274 1543 1562 1863 1962 Size: 533 426 202 113 269 19 301 99 Fragments arranged by size: 533 426 301 269 202 113 99 19 BsrBI CCG'CTC Cuts at: 0 147 1836 1962 Size: 147 1689 126 Fragments arranged by size: 1689 147 126 BsrFI r'CCGG_y Cuts at: 0 368 1962 Size: 368 1594 BssHII G'CGCG_C Cuts at: 0 1675 1962 Size: 1675 287 BssSI C'ACGA_G Cuts at: 0 1184 1962 Size: 1184 778 BstAPI GCAn_nnn'nTGC Cuts at: 0 219 1962 Size: 219 1743 BtgI C'CryG_G Cuts at: 0 72 107 153 1116 1229 1757 1808 1962 Size: 72 35 46 963 113 528 51 154 Fragments arranged by size: 963 528 154 113 72 51 46 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 194 1962 Size: 194 1768 BtsI GCAGTG_nn' Cuts at: 0 461 493 598 860 1068 1102 1405 1921 Size: 461 32 105 262 208 34 303 516 Cuts at: 1921 1962 Size: 41 Fragments arranged by size: 516 461 303 262 208 105 41 34 32 EaeI y'GGCC_r Cuts at: 0 281 535 845 936 1625 1754 1962 Size: 281 254 310 91 689 129 208 Fragments arranged by size: 689 310 281 254 208 129 91 EagI C'GGCC_G Cuts at: 0 535 1962 Size: 535 1427 EarI CTCTTCn'nnn_ Cuts at: 0 425 1277 1962 Size: 425 852 685 Fragments arranged by size: 852 685 425 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1813 1962 Size: 1813 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 269 647 1032 1621 1761 1962 Size: 269 378 385 589 140 201 Fragments arranged by size: 589 385 378 269 201 140 EcoO109I rG'GnC_Cy Cuts at: 0 216 257 377 579 1052 1347 1466 1467 Size: 216 41 120 202 473 295 119 1 Cuts at: 1467 1526 1962 Size: 59 436 Fragments arranged by size: 473 436 295 216 202 120 119 59 41 1 EcoRV GAT'ATC Cuts at: 0 1032 1962 Size: 1032 930 HaeII r_GCGC'y Cuts at: 0 456 1047 1652 1962 Size: 456 591 605 310 Fragments arranged by size: 605 591 456 310 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 940 972 1962 Size: 940 32 990 Fragments arranged by size: 990 940 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1175 1962 Size: 1175 787 MscI TGG'CCA Cuts at: 0 938 1627 1756 1962 Size: 938 689 129 206 Fragments arranged by size: 938 689 206 129 MspA1I CmG'CkG Cuts at: 0 231 334 983 1118 1392 1566 1578 1797 Size: 231 103 649 135 274 174 12 219 Cuts at: 1797 1810 1962 Size: 13 152 Fragments arranged by size: 649 274 231 219 174 152 135 103 13 12 NaeI GCC'GGC Cuts at: 0 370 1962 Size: 370 1592 NcoI C'CATG_G Cuts at: 0 107 153 1229 1757 1962 Size: 107 46 1076 528 205 Fragments arranged by size: 1076 528 205 107 46 NgoMIV G'CCGG_C Cuts at: 0 368 1962 Size: 368 1594 NspI r_CATG'y Cuts at: 0 1675 1962 Size: 1675 287 PasI CC'CwG_GG Cuts at: 0 885 1962 Size: 885 1077 PflMI CCAn_nnn'nTGG Cuts at: 0 945 1489 1962 Size: 945 544 473 Fragments arranged by size: 945 544 473 PmlI CAC'GTG Cuts at: 0 609 1962 Size: 609 1353 PspOMI G'GGCC_C Cuts at: 0 377 1347 1466 1962 Size: 377 970 119 496 Fragments arranged by size: 970 496 377 119 PvuII CAG'CTG Cuts at: 0 1797 1962 Size: 1797 165 SacII CC_GC'GG Cuts at: 0 1119 1811 1962 Size: 1119 692 151 Fragments arranged by size: 1119 692 151 SexAI A'CCwGG_T Cuts at: 0 1655 1962 Size: 1655 307 SfcI C'TryA_G Cuts at: 0 1891 1962 Size: 1891 71 SmaI CCC'GGG Cuts at: 0 262 501 679 1519 1949 1962 Size: 262 239 178 840 430 13 Fragments arranged by size: 840 430 262 239 178 13 SphI G_CATG'C Cuts at: 0 1675 1962 Size: 1675 287 StyI C'CwwG_G Cuts at: 0 79 107 153 1055 1229 1433 1644 1757 Size: 79 28 46 902 174 204 211 113 Cuts at: 1757 1962 Size: 205 Fragments arranged by size: 902 211 205 204 174 113 79 46 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 956 1581 1698 1962 Size: 956 625 117 264 Fragments arranged by size: 956 625 264 117 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1920 1952 1962 Size: 1920 32 10 Fragments arranged by size: 1920 32 10 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 160 1962 Size: 160 1802 XmaI C'CCGG_G Cuts at: 0 260 499 677 1517 1947 1962 Size: 260 239 178 840 430 15 Fragments arranged by size: 840 430 260 239 178 15 Enzymes that do cut: AarI AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbsI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I MmeI MscI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PmlI PspOMI PvuII SacII SexAI SfcI SmaI SphI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI