(Circular) (Six-base) MAPSORT of: pFN21AB5055.seq Check: 1944 from: 1 to: 6247 pFN21AB5055 6247 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2942 2942 Size: 6247 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3590 1937 Size: 1653 4594 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2606 2705 4310 5694 5962 1632 Size: 974 99 1605 1384 268 1917 Fragments arranged by size: 1917 1605 1384 974 268 99 AflII C'TTAA_G Cuts at: 820 1017 2186 3467 820 Size: 197 1169 1281 3600 Fragments arranged by size: 3600 1281 1169 197 AflIII A'CryG_T Cuts at: 1203 5146 6123 1203 Size: 3943 977 1327 Fragments arranged by size: 3943 1327 977 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 3355 4976 3355 Size: 1621 4626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6247 AlwNI CAG_nnn'CTG Cuts at: 2231 2357 2737 3990 5562 2231 Size: 126 380 1253 1572 2916 Fragments arranged by size: 2916 1572 1253 380 126 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 2297 2601 2839 3368 3556 3782 3934 6043 Size: 804 304 238 529 188 226 152 2109 Cuts at: 6043 1493 Size: 1697 Fragments arranged by size: 2109 1697 804 529 304 238 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 3572 1870 Size: 73 1629 4545 Fragments arranged by size: 4545 1629 73 BamHI G'GATC_C Cuts at: 3219 3577 3944 3219 Size: 358 367 5522 Fragments arranged by size: 5522 367 358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3419 3568 5023 618 Size: 325 217 627 1632 149 1455 1842 Fragments arranged by size: 1842 1632 1455 627 325 217 149 BanII G_rGCy'C Cuts at: 1391 3167 3566 1391 Size: 1776 399 4072 Fragments arranged by size: 4072 1776 399 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 928 Size: 971 660 4616 Fragments arranged by size: 4616 971 660 BbvCI CC'TCA_GC Cuts at: 2366 3275 3490 2366 Size: 909 215 5123 Fragments arranged by size: 5123 909 215 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2120 2154 4438 4472 1903 Size: 34 183 34 2284 34 3678 Fragments arranged by size: 3678 2284 183 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2438 4139 5349 2438 Size: 1701 1210 3336 Fragments arranged by size: 3336 1701 1210 BclI T'GATC_A Cuts at: 1548 2862 1548 Size: 1314 4933 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3143 4858 136 Size: 107 122 71 631 605 1471 1715 1525 Fragments arranged by size: 1715 1525 1471 631 605 122 107 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 3664 3664 Size: 6247 Bme1580I G_kGCm'C Cuts at: 1790 2033 4308 5464 1790 Size: 243 2275 1156 2573 Fragments arranged by size: 2573 2275 1156 243 BmgBI CAC'GTC Cuts at: 1318 2034 1318 Size: 716 5531 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3978 4936 442 Size: 416 53 83 399 24 2561 958 1753 Fragments arranged by size: 2561 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2467 4907 1239 Size: 1228 2440 2579 Fragments arranged by size: 2579 2440 1228 Bpu10I CC'TnA_GC Cuts at: 2366 3275 3490 2366 Size: 909 215 5123 Fragments arranged by size: 5123 909 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4910 882 Size: 617 3411 2219 Fragments arranged by size: 3411 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 4038 493 Size: 711 837 1997 2702 Fragments arranged by size: 2702 1997 837 711 BsaBI GATnn'nnATC Cuts at: 6241 6241 Size: 6247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3510 4054 4436 5071 Size: 53 83 186 936 1977 544 382 635 Cuts at: 5071 275 Size: 1451 Fragments arranged by size: 1977 1451 936 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3082 4680 5078 5352 5499 5910 3082 Size: 1598 398 274 147 411 3419 Fragments arranged by size: 3419 1598 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2898 2928 3455 3485 1178 Size: 30 165 30 1495 30 527 30 3940 Fragments arranged by size: 3940 1495 527 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2470 2522 1186 Size: 1284 52 4911 Fragments arranged by size: 4911 1284 52 BseYI C'CCAG_C Cuts at: 2231 5450 5946 2231 Size: 3219 496 2532 Fragments arranged by size: 3219 2532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3365 1163 Size: 573 1629 4045 Fragments arranged by size: 4045 1629 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4308 4393 5464 4308 Size: 85 1071 5091 Fragments arranged by size: 5091 1071 85 BsmI GAATG_Cn' Cuts at: 3758 3851 3758 Size: 93 6154 BsmBI CGTCTCn'nnnn_ Cuts at: 2079 2079 Size: 6247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 3167 3566 4308 4393 5464 1391 Size: 399 243 1134 399 742 85 1071 2174 Fragments arranged by size: 2174 1134 1071 742 399 399 243 85 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 2761 3156 4136 5866 2761 Size: 395 980 1730 3142 Fragments arranged by size: 3142 1730 980 395 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2942 3602 844 Size: 2098 660 3489 Fragments arranged by size: 3489 2098 660 BsrBI CCG'CTC Cuts at: 1394 1823 4134 1394 Size: 429 2311 3507 Fragments arranged by size: 3507 2311 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2253 2667 2917 3087 4742 4916 65 Size: 1183 1005 414 250 170 1655 174 1396 Fragments arranged by size: 1655 1396 1183 1005 414 250 174 170 BsrFI r'CCGG_y Cuts at: 1700 1810 4891 5078 1700 Size: 110 3081 187 2869 Fragments arranged by size: 3081 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2288 3045 96 Size: 2192 757 3298 Fragments arranged by size: 3298 2192 757 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 3010 794 Size: 2216 4031 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6247 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2903 3416 2248 Size: 655 513 5079 Fragments arranged by size: 5079 655 513 BstYI r'GATC_y Cuts at: 1972 3219 3577 3944 4329 4346 5798 5884 5896 Size: 1247 358 367 385 17 1452 86 12 Cuts at: 5896 6242 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1247 385 367 358 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2211 3261 513 Size: 552 939 207 1050 3499 Fragments arranged by size: 3499 1050 939 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 2136 2919 3048 3763 4557 4577 2136 Size: 783 129 715 794 20 3806 Fragments arranged by size: 3806 794 783 715 129 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2360 5254 809 Size: 507 1044 2894 1802 Fragments arranged by size: 2894 1802 1044 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2238 2661 Size: 54 1425 50 275 18 177 231 423 Cuts at: 2661 3694 4583 6212 8 Size: 1033 889 1629 43 Fragments arranged by size: 1629 1425 1033 889 423 275 231 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2526 2833 3473 4177 1353 Size: 725 448 307 640 704 3423 Fragments arranged by size: 3423 725 704 640 448 307 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4834 5208 5354 387 Size: 1064 138 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2467 2606 2705 4310 4907 5694 5962 Size: 393 835 139 99 1605 597 787 268 Cuts at: 5962 1239 Size: 1524 Fragments arranged by size: 1605 1524 835 787 597 393 268 139 99 EcoO109I rG'GnC_Cy Cuts at: 3144 3190 3144 Size: 46 6201 EcoRI G'AATT_C Cuts at: 2297 3556 3934 2297 Size: 1259 378 4610 Fragments arranged by size: 4610 1259 378 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 2255 3073 5394 1678 Size: 577 818 2321 2531 Fragments arranged by size: 2531 2321 818 577 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 3151 3183 3341 Size: 32 135 32 577 32 970 32 158 Cuts at: 3341 3373 4888 4920 4962 4994 1373 Size: 32 1515 32 42 32 2626 Fragments arranged by size: 2626 1515 970 577 158 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3591 3837 669 Size: 988 281 1653 246 3079 Fragments arranged by size: 3079 1653 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6247 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 3846 3846 Size: 6247 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3421 5361 5545 1305 Size: 2116 1940 184 2007 Fragments arranged by size: 2116 2007 1940 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 2663 10 Size: 54 1425 343 408 423 3594 Fragments arranged by size: 3594 1425 423 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2766 3155 3528 4205 4564 4723 Size: 648 1080 520 389 373 677 359 159 Cuts at: 4723 518 Size: 2042 Fragments arranged by size: 2042 1080 677 648 520 389 373 359 159 MspA1I CmG'CkG Cuts at: 1606 2734 3663 4340 5488 5733 1606 Size: 1128 929 677 1148 245 2120 Fragments arranged by size: 2120 1148 1128 929 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2211 3261 513 Size: 552 939 207 1050 3499 Fragments arranged by size: 3499 1050 939 552 207 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 3605 5150 1132 Size: 97 2376 1545 2229 Fragments arranged by size: 2376 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2120 2833 3325 1348 Size: 772 713 492 4270 Fragments arranged by size: 4270 772 713 492 PciI A'CATG_T Cuts at: 5146 5146 Size: 6247 PflMI CCAn_nnn'nTGG Cuts at: 1108 3112 1108 Size: 2004 4243 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 5829 5861 4316 Size: 32 1481 32 4702 Fragments arranged by size: 4702 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3437 1613 Size: 1824 4423 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PstI C_TGCA'G Cuts at: 830 2143 2575 2610 3599 830 Size: 1313 432 35 989 3478 Fragments arranged by size: 3478 1313 989 432 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 PvuII CAG'CTG Cuts at: 2734 2734 Size: 6247 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SapI GCTCTTCn'nnn_ Cuts at: 2078 2078 Size: 6247 SbfI CC_TGCA'GG Cuts at: 2575 3599 2575 Size: 1024 5223 ScaI AGT'ACT Cuts at: 4495 6207 4495 Size: 1712 4535 SexAI A'CCwGG_T Cuts at: 2145 2145 Size: 6247 SfcI C'TryA_G Cuts at: 826 1045 2139 2571 2606 3595 4730 5411 5602 Size: 219 1094 432 35 989 1135 681 191 Cuts at: 5602 826 Size: 1471 Fragments arranged by size: 1471 1135 1094 989 681 432 219 191 35 SfiI GGCCn_nnn'nGGCC Cuts at: 3143 3143 Size: 6247 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 3467 4351 5252 5514 820 Size: 197 926 243 1281 884 901 262 1553 Fragments arranged by size: 1553 1281 926 901 884 262 243 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 4171 5 Size: 47 4119 2081 Fragments arranged by size: 4119 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6247 StyI C'CwwG_G Cuts at: 513 1065 2004 2211 2373 2403 3132 3139 3261 Size: 552 939 207 162 30 729 7 122 Cuts at: 3261 3686 513 Size: 425 3074 Fragments arranged by size: 3074 939 729 552 425 207 162 122 30 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4274 4291 4444 4629 5074 325 Size: 1255 2694 17 153 185 445 1498 Fragments arranged by size: 2694 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2288 2868 3045 Size: 274 80 33 51 1443 311 580 177 Cuts at: 3045 3453 4493 6205 96 Size: 408 1040 1712 138 Fragments arranged by size: 1712 1443 1040 580 408 311 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 2898 2930 6207 6239 2283 Size: 32 583 32 3277 32 2291 Fragments arranged by size: 3277 2291 583 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2219 1316 Size: 903 5344 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3157 3332 3503 3157 Size: 175 171 5901 Fragments arranged by size: 5901 175 171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 4376 4376 Size: 6247 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfoI SrfI SwaI