Restriction map for
pFN21AB5769
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AspI BbeI BbrPI BciVI
BclI BfrI BsaAI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstZ17I ClaI
CpoI DraI DraIII Eco47III EcoRI
EcoRV EcoT22I FbaI FseI KasI
MluI MroI NarI NdeI NotI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SspBI SspI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 2057 GGTACC G^GTACC
AcsI 699 AAATTC [GA]^AATT[CT]
AcyI 194 GACGCC G[GA]^CG[CT]C
AhdI 505 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 699 AAATTC [GA]^AATT[CT]
AseI 2016 ATTAAT AT^TAAT
AsnI 2016 ATTAAT AT^TAAT
Asp700I 459 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2057 GGTACC G^GTACC
AspEI 505 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1494 GAGCTC G[AT]GC[AT]^C
AvaI 65 CTCGGG C^[CT]CG[GA]G
AviII 1535 TGCGCA TGC^GCA
AvrII 39 CCTAGG C^CTAGG
BbsI 502 GAAGAC GAAGAC(2/6)
BcgI 921 GCAGGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 916 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 832 AGATCT A^GATCT
BlnI 39 CCTAGG C^CTAGG
BlpI 1419 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1419 GCTGAGC GC^T[ACGT]AGC
BpuAI 502 GAAGAC GAAGAC(2/6)
BsaBI 2098 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 194 GACGCC G[GA]^CG[CT]C
BsaWI 1891 ACCGGA [AT]^CCGG[AT]
BsgI 1207 CTGCAC GTGCAG(16/14)
BsiHKAI 1494 GAGCTC G[AT]GC[AT]^C
BsmBI 751 GAGACG CGTCTC(1/5)
BsoBI 65 CTCGGG C^[CT]CG[GA]G
BsrDI 1686 CATTGC GCAATG(2/0)
BssHII 1283 GCGCGC G^CGCGC
BstAPI 2182 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 161 TTCGAA TT^CGAA
CelII 1419 GCTGAGC GC^T[ACGT]AGC
DrdI 1835 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 505 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 492 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1535 TGCGCA TGC^GCA
Hin1I 194 GACGCC G[GA]^CG[CT]C
HincII 1053 GTTAAC GT[CT]^[GA]AC
HindII 1053 GTTAAC GT[CT]^[GA]AC
HpaI 1053 GTTAAC GTT^AAC
KpnI 2057 GGTACC GGTAC^C
KspI 1412 CCGCGG CCGC^GG
MaeII 1202 ACGT A^CGT
MamI 2098 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 298 CAATTG C^AATTG
MunI 298 CAATTG C^AATTG
NaeI 1130 GCCGGC GCC^GGC
NcoI 767 CCATGG C^CATGG
NgoMIV 1130 GCCGGC G^CCGGC
NheI 379 GCTAGC G^CTAGC
NruI 1559 TCGCGA TCG^CGA
NspI 625 GCATGC [GA]CATG^[CT]
NspV 161 TTCGAA TT^CGAA
PpuMI 844 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2016 ATTAAT AT^TAAT
SacI 1494 GAGCTC GAGCT^C
SacII 1412 CCGCGG CCGC^GG
SbfI 1584 CCTGCAGG CCTGCA^GG
ScaI 1861 AGTACT AGT^ACT
SfuI 161 TTCGAA TT^CGAA
SphI 625 GCATGC GCATG^C
Sse8387I 1584 CCTGCAGG CCTGCA^GG
XbaI 1710 TCTAGA T^CTAGA
XmnI 459 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 814 CCTCAGG CC^T[ACGT]AGG
1106 CCTCAGG
BamHI 35 GGATCC G^GATCC
70 GGATCC
BbvCI 882 CCTCAGC CCTCAGC(-5/-2)
1479 CCTCAGC
BsaI 1566 GAGACC GGTCTC(1/5)
1607 GAGACC
BspMI 1151 ACCTGC ACCTGC(4/8)
123 GCAGGT
BssSI 1231 CACGAG CACGAG(-5/-1)
1255 CACGAG
BstEII 1794 GGTCACC G^GT[ACGT]ACC
1887 GGTCACC
BstPI 1794 GGTCACC G^GT[ACGT]ACC
1887 GGTCACC
BstXI 22 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1356 CCAAGGGAGTGG
Bsu36I 814 CCTCAGG CC^T[ACGT]AGG
1106 CCTCAGG
DsaI 767 CCATGG C^C[GA][CT]GG
1412 CCGCGG
EagI 1176 CGGCCG C^GGCCG
1347 CGGCCG
EclXI 1176 CGGCCG C^GGCCG
1347 CGGCCG
Eco52I 1176 CGGCCG C^GGCCG
1347 CGGCCG
Eco81I 814 CCTCAGG CC^T[ACGT]AGG
1106 CCTCAGG
EcoO65I 1794 GGTCACC G^GT[ACGT]ACC
1887 GGTCACC
HaeII 1098 AGCGCC [GA]GCGC^[CT]
1423 AGCGCC
HindIII 1762 AAGCTT A^AGCTT
1903 AAGCTT
MseI 1054 TTAA T^TAA
2017 TTAA
MslI 1878 CATTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1957 CATTTCCGTG
PvuII 2206 CAGCTG CAG^CTG
2215 CAGCTG
SexAI 578 ACCTGGT A^CC[AT]GGT
1610 ACCAGGT
SfaNI 649 GATGC GCATC(5/9)
2220 GATGC
Tru9I 1054 TTAA T^TAA
2017 TTAA
Tsp45I 1795 GTCAC ^GT[GC]AC
1888 GTCAC
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
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AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TfiI
TseI Tsp509I TspRI TthHB8I Van91I
XhoII