Restriction map for
pFN21AE1529
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstNI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MaeI
MaeII MfeI MflI MluI MluNI
MroI MscI MspI MunI MvaI
MwoI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 43 CGCG CG^CG
AcsI 215 AAATTT [GA]^AATT[CT]
ApoI 215 AAATTT [GA]^AATT[CT]
AvaII 70 GGACC G^G[AT]CC
BbsI 145 GTCTTC GAAGAC(2/6)
BcgI 45 CGATCCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 145 GTCTTC GAAGAC(2/6)
BsaBI 334 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 254 GTCTC GTCTC(1/5)
Bsp1407I 159 TGTACA T^GTACA
BspMI 51 ACCTGC ACCTGC(4/8)
BsrDI 436 GCAATG GCAATG(2/0)
BsrGI 159 TGTACA T^GTACA
BsrI 95 ACTGG ACTGG(1/-1)
BstEII 278 GGTCACC G^GT[ACGT]ACC
BstF5I 223 CATCC GGATG(2/0)
BstPI 278 GGTCACC G^GT[ACGT]ACC
BstUI 43 CGCG CG^CG
BstXI 104 CCACTATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 99 GCAAGC GC[ACGT]^[ACGT]GC
DraI 184 TTTAAA TTT^AAA
DraII 114 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 9 GAAGAG CTCTTC(1/4)
EcoO109I 114 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 278 GGTCACC G^GT[ACGT]ACC
FokI 223 CATCC GGATG(9/13)
HaeII 3 GGCGCT [GA]GCGC^[CT]
HaeIII 116 GGCC GG^CC
HphI 280 TCACC GGTGA(8/7)
Ksp632I 9 GAAGAG CTCTTC(1/4)
MamI 334 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 185 TTAA T^TAA
MslI 105 CACTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 65 CAGCTG C[AC]G^C[GT]G
MvnI 43 CGCG CG^CG
NdeI 315 CATATG CA^TATG
NlaIII 409 CATG CATG^
NlaIV 114 GGGGCC GG[ACGT]^[ACGT]CC
NspI 408 ACATGC [GA]CATG^[CT]
PvuII 65 CAGCTG CAG^CTG
SfcI 37 CTACAG C^T[GA][CT]AG
SspBI 159 TGTACA T^GTACA
TaqI 257 TCGA T^CGA
TfiI 15 GATTC G^A[AT]TC
Tru9I 185 TTAA T^TAA
TthHB8I 257 TCGA T^CGA
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 160 GTAC GT^AC
358 GTAC
AluI 66 AGCT AG^CT
285 AGCT
AlwI 46 GATCC GGATC(4/5)
334 GATCC
Cfr13I 70 GGACC G^G[ACGT]CC
115 GGGCC
MaeIII 270 GTCAC ^GT[ACGT]AC
279 GTCAC
MboII 9 GAAGA GAAGA(8/7)
146 TCTTC
PleI 423 GACTC GAGTC(4/5)
143 GAGTC
RsaI 160 GTAC GT^AC
358 GTAC
Sau96I 70 GGACC G^G[ACGT]CC
115 GGGCC
Tsp45I 270 GTCAC ^GT[GC]AC
279 GTCAC
Tsp509I 26 AATT ^AATT
216 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
CfoI DdeI DpnII HhaI HinP1I
HinfI MboI MnlI NdeII Sau3AI
TspRI