Restriction map for
pFN21AE1525
(Circular) (Six-base) MAPSORT of: pFN21AE1525.seq Check: 2466 from: 1 to: 5476
pFN21AE1525 5476 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 2775 3603 3976 1563
Size: 1212 828 373 3063
Fragments arranged by size:
3063 1212 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2314 2333 2716 3539 4923 5191 1632
Size: 682 19 383 823 1384 268 1917
Fragments arranged by size:
1917 1384 823 682 383 268 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 2153 4375 5352 1203
Size: 950 2222 977 1327
Fragments arranged by size:
2222 1327 977 950
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 2560 4205 2560
Size: 1645 3831
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 2337 3219 4791 2337
Size: 882 1572 3022
Fragments arranged by size:
3022 1572 882
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2227 2255 2785 3011 3163 5272 1493
Size: 734 28 530 226 152 2109 1697
Fragments arranged by size:
2109 1697 734 530 226 152 28
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2801 1870
Size: 73 858 4545
Fragments arranged by size:
4545 858 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2300 2333 2300
Size: 33 5443
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2397 2797 4252 618
Size: 325 217 627 610 400 1455 1842
Fragments arranged by size:
1842 1455 627 610 400 325 217
BanII G_rGCy'C
Cuts at: 1391 2571 2795 1391
Size: 1180 224 4072
Fragments arranged by size:
4072 1180 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 2320 2517 928
Size: 971 369 52 197 3887
Fragments arranged by size:
3887 971 369 197 52
BbvCI CC'TCA_GC
Cuts at: 2361 2361
Size: 5476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 3537 4693 1790
Size: 1747 1156 2573
Fragments arranged by size:
2573 1747 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2425 3207 4165
Size: 416 53 83 399 24 1008 782 958
Cuts at: 4165 442
Size: 1753
Fragments arranged by size:
1753 1008 958 782 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 4136 1239
Size: 807 2090 2579
Fragments arranged by size:
2579 2090 807
Bpu10I CC'TnA_GC
Cuts at: 2361 2754 2361
Size: 393 5083
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2444 3601 4466 4764 2444
Size: 1157 865 298 3156
Fragments arranged by size:
3156 1157 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2104 4139 882
Size: 617 605 2035 2219
Fragments arranged by size:
2219 2035 617 605
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3283 3665 4300 275
Size: 53 83 186 936 1750 382 635 1451
Fragments arranged by size:
1750 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2127 2157 2471 2501 1178
Size: 30 165 30 724 30 314 30 4153
Fragments arranged by size:
4153 724 314 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5476
BseYI C'CCAG_C
Cuts at: 4679 5175 4679
Size: 496 4980
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4903
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3537 3622 4693 3537
Size: 85 1071 4320
Fragments arranged by size:
4320 1071 85
BsmI GAATG_Cn'
Cuts at: 2254 2987 3080 2254
Size: 733 93 4650
Fragments arranged by size:
4650 733 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2571 2795 3537 3622 4693 1391
Size: 399 781 224 742 85 1071 2174
Fragments arranged by size:
2174 1071 781 742 399 224 85
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2680 2831 844
Size: 1836 151 3489
Fragments arranged by size:
3489 1836 151
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 1394
Size: 429 1540 3507
Fragments arranged by size:
3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 2424 96
Size: 2328 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471
Size: 834 367 385 17 1452 86 12 346
Cuts at: 5471 1972
Size: 1977
Fragments arranged by size:
1977 1452 834 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2094 2393 2094
Size: 299 5177
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2114 2401 513
Size: 552 939 12 98 287 3588
Fragments arranged by size:
3588 939 552 287 98 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2131 2780 2131
Size: 649 4827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2923 3812 5441
Size: 54 1425 50 275 18 1093 889 1629
Cuts at: 5441 8
Size: 43
Fragments arranged by size:
1629 1425 1093 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2393 3406 1353
Size: 861 179 1013 3423
Fragments arranged by size:
3423 1013 861 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2314 2333 2716 3539 4136 4923
Size: 393 414 268 19 383 823 597 787
Cuts at: 4923 5191 1239
Size: 268 1524
Fragments arranged by size:
1524 823 787 597 414 393 383 268
268 19
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 5476
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 4623 1678
Size: 2945 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 2658 2690 4117
Size: 32 135 32 974 32 80 32 1427
Cuts at: 4117 4149 4191 4223 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1427 974 135 80 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2387 4590 4774 1305
Size: 1082 2203 184 2007
Fragments arranged by size:
2203 2007 1082 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3654
Fragments arranged by size:
3654 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2551 2892 3569 4717 4962 1606
Size: 945 341 677 1148 245 2120
Fragments arranged by size:
2120 1148 945 677 341 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2016 2114 2401 513
Size: 552 939 12 98 287 3588
Fragments arranged by size:
3588 939 552 287 98 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2157 2834 4379 1132
Size: 97 928 677 1545 2229
Fragments arranged by size:
2229 1545 928 677 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 2153 4375 2153
Size: 2222 3254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2193 1108
Size: 1085 4391
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 2442 3046 2442
Size: 604 4872
PstI C_TGCA'G
Cuts at: 830 2828 830
Size: 1998 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 2465 2609 3724 5436 2465
Size: 144 1115 1712 2505
Fragments arranged by size:
2505 1712 1115 144
SfcI C'TryA_G
Cuts at: 826 1045 2824 3959 4640 4831 826
Size: 219 1779 1135 681 191 1471
Fragments arranged by size:
1779 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2459 3580 4481 4743 820
Size: 197 926 516 1121 901 262 1553
Fragments arranged by size:
1553 1121 926 901 516 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 2127 2242 3400 5
Size: 47 2075 115 1158 2081
Fragments arranged by size:
2081 2075 1158 115 47
StuI AGG'CCT
Cuts at: 1517 2354 1517
Size: 837 4639
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2114 2401 2915 513
Size: 552 939 12 98 287 514 3074
Fragments arranged by size:
3074 939 552 514 287 98 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2463 2607
Size: 274 80 33 51 1443 447 39 144
Cuts at: 2607 3722 5434 96
Size: 1115 1712 138
Fragments arranged by size:
1712 1443 1115 447 274 144 138 80
51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2471 2503 5436 5468 2127
Size: 32 312 32 2933 32 2135
Fragments arranged by size:
2933 2135 312 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 2168 2729 3605 2168
Size: 561 876 4039
Fragments arranged by size:
4039 876 561
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI