################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AfaI 340 GTAC GT^AC Aor51HI 170 AGCGCT AGC^GCT Asp700I 231 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 225 GAGCTC G[AT]GC[AT]^C BamHI 56 GGATCC G^GATCC BbvCI 502 CCTCAGC CCTCAGC(-5/-2) BcnI 188 CCCGG CC^[GC]GG BpmI 484 CTCCAG CTGGAG(16/14) BsaAI 292 TACGTG [CT]AC^GT[GA] BsaI 68 GGTCTC GGTCTC(1/5) BsgI 539 CTGCAC GTGCAG(16/14) BsiHKAI 225 GAGCTC G[AT]GC[AT]^C BspMI 394 GCAGGT ACCTGC(4/8) BsrBI 259 GAGCGG CCGCTC(-3/-3) BsrDI 200 CATTGC GCAATG(2/0) BssHII 245 GCGCGC G^CGCGC BstXI 363 CCAGACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 296 TGGCCG [CT]^GGCC[GA] Eco47III 170 AGCGCT AGC^GCT HapII 189 CCGG C^CGG HpaII 189 CCGG C^CGG KspI 211 CCGCGG CCGC^GG MaeII 293 ACGT A^CGT MslI 288 CACTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 189 CCGG C^CGG NciI 188 CCCGG CC^[GC]GG PflMI 580 CCAGTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 340 GTAC GT^AC SacI 225 GAGCTC GAGCT^C SacII 211 CCGCGG CCGC^GG SfaNI 192 GCATC GCATC(5/9) SspI 604 AATATT AAT^ATT Van91I 580 CCAGTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 231 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 590 GAATTT [GA]^AATT[CT] 724 GAATTT AcyI 14 GGCGTC G[GA]^CG[CT]C 324 GGCGTC ApoI 590 GAATTT [GA]^AATT[CT] 724 GAATTT BglI 207 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 210 GCCGCGGCGGC BsaHI 14 GGCGTC G[GA]^CG[CT]C 324 GGCGTC BseRI 253 GAGGAG GAGGAG(10/8) 256 GAGGAG BsmFI 124 GGGAC GGGAC(10/14) 160 GGGAC BstAPI 394 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 801 GCACAAGTTGC BstF5I 193 CATCC GGATG(2/0) 22 GGATG BstYI 56 GGATCC [GA]^GATC[CT] 735 AGATCC DsaI 211 CCGCGG C^C[GA][CT]GG 308 CCGTGG FokI 193 CATCC GGATG(9/13) 22 GGATG HaeII 50 GGCGCT [GA]GCGC^[CT] 170 AGCGCT HgaI 15 GCGTC GACGC(5/10) 325 GCGTC Hin1I 14 GGCGTC G[GA]^CG[CT]C 324 GGCGTC HinfI 443 GAATC G^A[ACGT]TC 564 GAATC MboII 661 GAAGA GAAGA(8/7) 733 GAAGA MflI 56 GGATCC [GA]^GATC[CT] 735 AGATCC MspA1I 211 CCGCGG C[AC]G^C[GT]G 299 CCGCTG NspI 314 GCATGC [GA]CATG^[CT] 474 GCATGC PstI 83 CTGCAG CTGCA^G 174 CTGCAG SfcI 83 CTGCAG C^T[GA][CT]AG 174 CTGCAG SphI 314 GCATGC GCATG^C 474 GCATGC TfiI 443 GAATC G^A[AT]TC 564 GAATC XhoII 56 GGATCC [GA]^GATC[CT] 735 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AccII AciI AluI AlwI BanI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI HaeIII HhaI HinP1I ItaI MboI MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI TaqI Tru9I TseI Tsp509I TspRI TthHB8I