(Circular) (Six-base) MAPSORT of: pFN21AE1534.seq Check: 8407 from: 1 to: 4987 pFN21AE1534 4987 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2797 278 Size: 53 83 186 936 1261 2468 Fragments arranged by size: 2468 1261 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2330 1937 Size: 393 4594 Acc65I G'GTAC_C Cuts at: 2308 2308 Size: 4987 AclI AA'CG_TT Cuts at: 1563 3114 3487 1563 Size: 1551 373 3063 Fragments arranged by size: 3063 1551 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3050 4434 4702 1632 Size: 1418 1384 268 1917 Fragments arranged by size: 1917 1418 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4790 AflIII A'CryG_T Cuts at: 1203 3886 4863 1203 Size: 2683 977 1327 Fragments arranged by size: 2683 1327 977 AgeI A'CCGG_T Cuts at: 3818 3818 Size: 4987 AhdI GACnn_n'nnGTC Cuts at: 3716 3716 Size: 4987 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4987 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4987 AlwNI CAG_nnn'CTG Cuts at: 2730 4302 2730 Size: 1572 3415 ApaLI G'TGCA_C Cuts at: 3044 4200 3044 Size: 1156 3831 ApoI r'AATT_y Cuts at: 1493 2281 2296 2522 2674 4783 1493 Size: 788 15 226 152 2109 1697 Fragments arranged by size: 2109 1697 788 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 3541 160 Size: 872 2509 1606 Fragments arranged by size: 2509 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4987 AvaI C'yCGr_G Cuts at: 1870 1943 2312 1870 Size: 73 369 4545 Fragments arranged by size: 4545 369 73 BamHI G'GATC_C Cuts at: 2317 2684 2317 Size: 367 4620 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2308 3763 618 Size: 325 217 627 521 1455 1842 Fragments arranged by size: 1842 1455 627 521 325 217 BanII G_rGCy'C Cuts at: 1391 2306 1391 Size: 915 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4016 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3178 3212 1903 Size: 34 1241 34 3678 Fragments arranged by size: 3678 1241 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2879 4089 2879 Size: 1210 3777 BclI T'GATC_A Cuts at: 1548 1548 Size: 4987 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3598 136 Size: 107 122 71 631 605 1926 1525 Fragments arranged by size: 1926 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4982 4982 Size: 4987 BlpI GC'TnA_GC Cuts at: 2404 2404 Size: 4987 Bme1580I G_kGCm'C Cuts at: 1790 3048 4204 1790 Size: 1258 1156 2573 Fragments arranged by size: 2573 1258 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4987 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2718 3676 442 Size: 416 53 83 399 24 1301 958 1753 Fragments arranged by size: 1753 1301 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4987 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 4987 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3647 1239 Size: 2408 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3112 3977 4275 3112 Size: 865 298 3824 Fragments arranged by size: 3824 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3650 882 Size: 617 2151 2219 Fragments arranged by size: 2219 2151 617 BsaAI yAC'GTr Cuts at: 493 1204 2778 493 Size: 711 1574 2702 Fragments arranged by size: 2702 1574 711 BsaBI GATnn'nnATC Cuts at: 4981 4981 Size: 4987 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2794 3176 3811 275 Size: 53 83 186 936 1261 382 635 1451 Fragments arranged by size: 1451 1261 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3420 3818 4092 4239 4650 3420 Size: 398 274 147 411 3757 Fragments arranged by size: 3757 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4762 Fragments arranged by size: 4762 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4987 BseYI C'CCAG_C Cuts at: 4190 4686 4190 Size: 496 4491 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2437 3198 4226 1540 Size: 275 186 436 761 1028 2301 Fragments arranged by size: 2301 1028 761 436 275 186 BsiHKAI G_wGCw'C Cuts at: 3048 3133 4204 3048 Size: 85 1071 3831 Fragments arranged by size: 3831 1071 85 BsmI GAATG_Cn' Cuts at: 2498 2591 2498 Size: 93 4894 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2306 3048 3133 4204 1391 Size: 399 516 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 516 399 85 BspEI T'CCGG_A Cuts at: 4650 4650 Size: 4987 BspHI T'CATG_A Cuts at: 2876 4606 2876 Size: 1730 3257 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2342 844 Size: 1498 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2874 1394 Size: 429 1051 3507 Fragments arranged by size: 3507 1051 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3482 3656 65 Size: 1183 2234 174 1396 Fragments arranged by size: 2234 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3631 3818 1700 Size: 110 1821 187 2869 Fragments arranged by size: 2869 1821 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4987 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4987 BssSI C'ACGA_G Cuts at: 3047 4059 3047 Size: 1012 3975 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4987 BstBI TT'CG_AA Cuts at: 3791 3791 Size: 4987 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4987 BstYI r'GATC_y Cuts at: 1972 2317 2684 3069 3086 4538 4624 4636 4982 Size: 345 367 385 17 1452 86 12 346 Cuts at: 4982 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 345 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4435 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4248 Fragments arranged by size: 4248 683 56 BtsI GCAGTG_nn' Cuts at: 2127 2503 3297 3317 2127 Size: 376 794 20 3797 Fragments arranged by size: 3797 794 376 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4987 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4952 DraI TTT'AAA Cuts at: 2291 2291 Size: 4987 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3994 809 Size: 507 2678 1802 Fragments arranged by size: 2678 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2434 3323 4952 Size: 54 1425 50 275 18 604 889 1629 Cuts at: 4952 8 Size: 43 Fragments arranged by size: 1629 1425 889 604 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2434 1537 Size: 275 622 4090 Fragments arranged by size: 4090 622 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2917 1353 Size: 1564 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3574 3948 4094 387 Size: 1064 138 1985 374 146 1280 Fragments arranged by size: 1985 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3050 3647 4434 4702 1239 Size: 393 1418 597 787 268 1524 Fragments arranged by size: 1524 1418 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2105 2105 Size: 4987 EcoRI G'AATT_C Cuts at: 2296 2674 2296 Size: 378 4609 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2270 2270 Size: 4987 FspI TGC'GCA Cuts at: 3493 3493 Size: 4987 HaeII r_GCGC'y Cuts at: 1678 4134 1678 Size: 2456 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3628 3660 3702 3734 1373 Size: 32 135 32 2056 32 42 32 2626 Fragments arranged by size: 2626 2056 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2331 2577 669 Size: 988 281 393 246 3079 Fragments arranged by size: 3079 988 393 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4987 HpaI GTT'AAC Cuts at: 2577 2577 Size: 4987 KpnI G_GTAC'C Cuts at: 2312 2312 Size: 4987 MfeI C'AATT_G Cuts at: 2586 2586 Size: 4987 MluI A'CGCG_T Cuts at: 4863 4863 Size: 4987 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4101 4285 1305 Size: 2796 184 2007 Fragments arranged by size: 2796 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3165 Fragments arranged by size: 3165 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2945 3304 3463 518 Size: 648 843 936 359 159 2042 Fragments arranged by size: 2042 936 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2403 3080 4228 4473 1606 Size: 797 677 1148 245 2120 Fragments arranged by size: 2120 1148 797 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4435 NdeI CA'TA_TG Cuts at: 387 387 Size: 4987 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4987 NotI GC'GGCC_GC Cuts at: 2434 2434 Size: 4987 NspI r_CATG'y Cuts at: 1132 1229 2345 3890 1132 Size: 97 1116 1545 2229 Fragments arranged by size: 2229 1545 1116 97 PasI CC'CwG_GG Cuts at: 1348 2108 1348 Size: 760 4227 PciI A'CATG_T Cuts at: 3886 3886 Size: 4987 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4987 PmeI GTTT'AAAC Cuts at: 2291 2291 Size: 4987 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3056 3088 4569 4601 3056 Size: 32 1481 32 3442 Fragments arranged by size: 3442 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4987 PsiI TTA'TAA Cuts at: 2258 2557 2258 Size: 299 4688 PstI C_TGCA'G Cuts at: 830 2339 830 Size: 1509 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4987 SalI G'TCGA_C Cuts at: 1936 2329 1936 Size: 393 4594 SbfI CC_TGCA'GG Cuts at: 2339 2339 Size: 4987 ScaI AGT'ACT Cuts at: 3235 4947 3235 Size: 1712 3275 SfcI C'TryA_G Cuts at: 826 1045 2025 2052 2335 3470 4151 4342 826 Size: 219 980 27 283 1135 681 191 1471 Fragments arranged by size: 1471 1135 980 681 283 219 191 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4987 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4987 SmaI CCC'GGG Cuts at: 1872 2314 1872 Size: 442 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3091 3992 4254 820 Size: 197 926 1148 901 262 1553 Fragments arranged by size: 1553 1148 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2778 493 Size: 2285 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4987 SphI G_CATG'C Cuts at: 1132 2345 1132 Size: 1213 3774 SspI AAT'ATT Cuts at: 5 52 2911 5 Size: 47 2859 2081 Fragments arranged by size: 2859 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4987 StyI C'CwwG_G Cuts at: 513 1065 2031 2426 513 Size: 552 966 395 3074 Fragments arranged by size: 3074 966 552 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3014 3031 3184 3369 3814 325 Size: 1255 1434 17 153 185 445 1498 Fragments arranged by size: 1498 1434 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3233 4945 96 Size: 274 80 33 51 1443 1256 1712 138 Fragments arranged by size: 1712 1443 1256 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4947 4979 4947 Size: 32 4955 Tth111I GACn'n_nGTC Cuts at: 1316 2091 1316 Size: 775 4212 XbaI T'CTAG_A Cuts at: 2323 2323 Size: 4987 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4987 XmaI C'CCGG_G Cuts at: 1870 2312 1870 Size: 442 4545 XmnI GAAnn'nnTTC Cuts at: 3116 3116 Size: 4987 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2795 276 Size: 53 83 186 936 1261 2468 Fragments arranged by size: 2468 1261 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI