################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbsI BcgI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AspHI 199 GAGCTC G[AT]GC[AT]^C BamHI 170 GGATCC G^GATCC BanII 199 GAGCTC G[GA]GC[CT]^C BbvCI 136 CCTCAGC CCTCAGC(-5/-2) BbvI 268 GCTGC GCAGC(8/12) BciVI 259 GGATAC GTATCC(6/5) BglI 22 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 199 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 199 GAGCTC G[AT]GC[AT]^C Bsp1286I 199 GAGCTC G[AGT]GC[ACT]^C BssKI 27 CCTGG ^CC[ACGT]GG BssSI 196 CACGAG CACGAG(-5/-1) BstF5I 24 CATCC GGATG(2/0) BstNI 27 CCTGG CC^[AT]GG BstYI 170 GGATCC [GA]^GATC[CT] CfoI 294 GCGC GCG^C Cfr13I 302 GGGCC G^G[ACGT]CC DsaI 248 CCATGG C^C[GA][CT]GG EaeI 29 TGGCCG [CT]^GGCC[GA] EarI 3 GAAGAG CTCTTC(1/4) EcoRII 27 CCTGG ^CC[AT]GG EcoT14I 248 CCATGG C^C[AT][AT]GG FokI 24 CATCC GGATG(9/13) HaeII 293 AGCGCC [GA]GCGC^[CT] HhaI 294 GCGC GCG^C HinP1I 294 GCGC G^CGC Ksp632I 3 GAAGAG CTCTTC(1/4) MaeIII 46 GTAAC ^GT[ACGT]AC MboII 3 GAAGA GAAGA(8/7) MflI 170 GGATCC [GA]^GATC[CT] MvaI 27 CCTGG CC^[AT]GG NcoI 248 CCATGG C^CATGG SacI 199 GAGCTC GAGCT^C SapI 3 GAAGAGC GCTCTTC(1/4) Sau96I 302 GGGCC G^G[ACGT]CC ScrFI 27 CCTGG CC^[ACGT]GG SfcI 225 CTACAG C^T[GA][CT]AG StyI 248 CCATGG C^C[AT][AT]GG TseI 268 GCTGC G^C[AT]GC XhoII 170 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 32 CCGC CCGC(-3/-1) 40 GCGG AluI 153 AGCT AG^CT 200 AGCT BsaJI 248 CCATGG C^C[ACGT][ACGT]GG 297 CCGAGG DpnII 12 GATC ^GATC 171 GATC Fnu4HI 31 GCCGC GC^[ACGT]GC 268 GCTGC HaeIII 30 GGCC GG^CC 303 GGCC HinfI 61 GAATC G^A[ACGT]TC 79 GAATC ItaI 31 GCCGC GC^[ACGT]GC 268 GCTGC MboI 12 GATC ^GATC 171 GATC MseI 92 TTAA T^TAA 104 TTAA MwoI 22 GCCATCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 31 GCCGCCTTAGC NdeII 12 GATC ^GATC 171 GATC NlaIV 170 GGATCC GG[ACGT]^[ACGT]CC 301 GGGGCC Sau3AI 12 GATC ^GATC 171 GATC TfiI 61 GAATC G^A[AT]TC 79 GAATC Tru9I 92 TTAA T^TAA 104 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AlwI DdeI MnlI NlaIII