(Circular) (Six-base) MAPSORT of: pFN21AE5109.seq Check: 9613 from: 1 to: 5182 pFN21AE5109 5182 bp With 182 enzymes: SgfI * January 30, 2014 17:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2992 278 Size: 53 83 186 936 1456 2468 Fragments arranged by size: 2468 1456 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2525 1937 Size: 588 4594 Acc65I G'GTAC_C Cuts at: 2503 2503 Size: 5182 AclI AA'CG_TT Cuts at: 1563 3309 3682 1563 Size: 1746 373 3063 Fragments arranged by size: 3063 1746 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 3245 4629 4897 1632 Size: 461 1152 1384 268 1917 Fragments arranged by size: 1917 1384 1152 461 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4985 AflIII A'CryG_T Cuts at: 1203 4081 5058 1203 Size: 2878 977 1327 Fragments arranged by size: 2878 1327 977 AgeI A'CCGG_T Cuts at: 4013 4013 Size: 5182 AhdI GACnn_n'nnGTC Cuts at: 3911 3911 Size: 5182 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5182 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2388 1184 Size: 1204 3978 AlwNI CAG_nnn'CTG Cuts at: 2925 4497 2925 Size: 1572 3610 ApaLI G'TGCA_C Cuts at: 3239 4395 3239 Size: 1156 4026 ApoI r'AATT_y Cuts at: 1493 2491 2717 2869 4978 1493 Size: 998 226 152 2109 1697 Fragments arranged by size: 2109 1697 998 226 152 AseI AT'TA_AT Cuts at: 160 1032 3736 160 Size: 872 2704 1606 Fragments arranged by size: 2704 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5182 AvaI C'yCGr_G Cuts at: 1870 1943 2507 1870 Size: 73 564 4545 Fragments arranged by size: 4545 564 73 AvrII C'CTAG_G Cuts at: 2044 2044 Size: 5182 BamHI G'GATC_C Cuts at: 2512 2879 2512 Size: 367 4815 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2503 3958 618 Size: 325 217 627 252 464 1455 1842 Fragments arranged by size: 1842 1455 627 464 325 252 217 BanII G_rGCy'C Cuts at: 1391 2059 2501 1391 Size: 668 442 4072 Fragments arranged by size: 4072 668 442 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3373 3407 1903 Size: 34 1436 34 3678 Fragments arranged by size: 3678 1436 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3074 4284 3074 Size: 1210 3972 BclI T'GATC_A Cuts at: 1548 1548 Size: 5182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3793 136 Size: 107 122 71 631 605 2121 1525 Fragments arranged by size: 2121 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5177 5177 Size: 5182 BlpI GC'TnA_GC Cuts at: 2599 2599 Size: 5182 Bme1580I G_kGCm'C Cuts at: 1790 2044 3243 4399 1790 Size: 254 1199 1156 2573 Fragments arranged by size: 2573 1199 1156 254 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5182 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2326 2913 3871 Size: 416 53 83 399 24 909 587 958 Cuts at: 3871 442 Size: 1753 Fragments arranged by size: 1753 958 909 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 2058 3842 1239 Size: 799 20 1784 2579 Fragments arranged by size: 2579 1784 799 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3307 4172 4470 3307 Size: 865 298 4019 Fragments arranged by size: 4019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3845 882 Size: 617 2346 2219 Fragments arranged by size: 2346 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2973 493 Size: 711 1769 2702 Fragments arranged by size: 2702 1769 711 BsaBI GATnn'nnATC Cuts at: 5176 5176 Size: 5182 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2989 3371 4006 275 Size: 53 83 186 936 1456 382 635 1451 Fragments arranged by size: 1456 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3615 4013 4287 4434 4845 3615 Size: 398 274 147 411 3952 Fragments arranged by size: 3952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2011 2041 1178 Size: 30 165 30 608 30 4319 Fragments arranged by size: 4319 608 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5182 BseYI C'CCAG_C Cuts at: 2414 4385 4881 2414 Size: 1971 496 2715 Fragments arranged by size: 2715 1971 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 1163 Size: 573 426 4183 Fragments arranged by size: 4183 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2632 3393 4421 1540 Size: 275 186 631 761 1028 2301 Fragments arranged by size: 2301 1028 761 631 275 186 BsiHKAI G_wGCw'C Cuts at: 2059 3243 3328 4399 2059 Size: 1184 85 1071 2842 Fragments arranged by size: 2842 1184 1071 85 BsmI GAATG_Cn' Cuts at: 2693 2786 2693 Size: 93 5089 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2059 2501 3243 3328 4399 1391 Size: 399 254 15 442 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 442 399 254 85 15 BspEI T'CCGG_A Cuts at: 4845 4845 Size: 5182 BspHI T'CATG_A Cuts at: 3071 4801 3071 Size: 1730 3452 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2095 2537 844 Size: 1251 442 3489 Fragments arranged by size: 3489 1251 442 BsrBI CCG'CTC Cuts at: 1394 1823 3069 1394 Size: 429 1246 3507 Fragments arranged by size: 3507 1246 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3677 3851 65 Size: 1183 2429 174 1396 Fragments arranged by size: 2429 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3826 4013 1700 Size: 110 2016 187 2869 Fragments arranged by size: 2869 2016 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5182 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5182 BssSI C'ACGA_G Cuts at: 3242 4254 3242 Size: 1012 4170 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5182 BstBI TT'CG_AA Cuts at: 2068 3986 2068 Size: 1918 3264 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5182 BstXI CCAn_nnnn'nTGG Cuts at: 2174 2174 Size: 5182 BstYI r'GATC_y Cuts at: 1972 2512 2879 3264 3281 4733 4819 4831 5177 Size: 540 367 385 17 1452 86 12 346 Cuts at: 5177 1972 Size: 1977 Fragments arranged by size: 1977 1452 540 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3691 Fragments arranged by size: 3691 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2288 487 Size: 683 56 1062 3381 Fragments arranged by size: 3381 1062 683 56 BtsI GCAGTG_nn' Cuts at: 2698 3492 3512 2698 Size: 794 20 4368 Fragments arranged by size: 4368 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5182 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5147 DraI TTT'AAA Cuts at: 2486 2486 Size: 5182 DraIII CAC_nnn'GTG Cuts at: 2443 2443 Size: 5182 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4189 809 Size: 507 2873 1802 Fragments arranged by size: 2873 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2629 3518 5147 Size: 54 1425 50 275 18 799 889 1629 Cuts at: 5147 8 Size: 43 Fragments arranged by size: 1629 1425 889 799 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2629 1537 Size: 275 817 4090 Fragments arranged by size: 4090 817 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 3112 1353 Size: 957 802 3423 Fragments arranged by size: 3423 957 802 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3769 4143 4289 387 Size: 1064 138 2180 374 146 1280 Fragments arranged by size: 2180 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2057 2057 Size: 5182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2058 2093 3245 3842 4629 4897 Size: 393 406 20 35 1152 597 787 268 Cuts at: 4897 1239 Size: 1524 Fragments arranged by size: 1524 1152 787 597 406 393 268 35 20 EcoO109I rG'GnC_Cy Cuts at: 2320 2320 Size: 5182 EcoRI G'AATT_C Cuts at: 2491 2869 2491 Size: 378 4804 FspI TGC'GCA Cuts at: 3688 3688 Size: 5182 HaeII r_GCGC'y Cuts at: 1678 4329 1678 Size: 2651 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3823 3855 3897 3929 1373 Size: 32 135 32 2251 32 42 32 2626 Fragments arranged by size: 2626 2251 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2526 2772 669 Size: 988 281 588 246 3079 Fragments arranged by size: 3079 988 588 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5182 HpaI GTT'AAC Cuts at: 2772 2772 Size: 5182 KpnI G_GTAC'C Cuts at: 2507 2507 Size: 5182 MfeI C'AATT_G Cuts at: 2781 2781 Size: 5182 MluI A'CGCG_T Cuts at: 5058 5058 Size: 5182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4296 4480 1305 Size: 2991 184 2007 Fragments arranged by size: 2991 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3360 Fragments arranged by size: 3360 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 3140 3499 3658 518 Size: 648 1189 785 359 159 2042 Fragments arranged by size: 2042 1189 785 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2598 3275 4423 4668 1606 Size: 992 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3691 Fragments arranged by size: 3691 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5182 NotI GC'GGCC_GC Cuts at: 2629 2629 Size: 5182 NspI r_CATG'y Cuts at: 1132 1229 2540 4085 1132 Size: 97 1311 1545 2229 Fragments arranged by size: 2229 1545 1311 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5182 PciI A'CATG_T Cuts at: 4081 4081 Size: 5182 PflMI CCAn_nnn'nTGG Cuts at: 1108 2472 1108 Size: 1364 3818 PmeI GTTT'AAAC Cuts at: 2486 2486 Size: 5182 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3251 3283 4764 4796 3251 Size: 32 1481 32 3637 Fragments arranged by size: 3637 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5182 PsiI TTA'TAA Cuts at: 2752 2752 Size: 5182 PstI C_TGCA'G Cuts at: 830 2296 2534 830 Size: 1466 238 3478 Fragments arranged by size: 3478 1466 238 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5182 SacI G_AGCT'C Cuts at: 2059 2059 Size: 5182 SalI G'TCGA_C Cuts at: 1936 2524 1936 Size: 588 4594 SbfI CC_TGCA'GG Cuts at: 2534 2534 Size: 5182 ScaI AGT'ACT Cuts at: 3430 5142 3430 Size: 1712 3470 SfcI C'TryA_G Cuts at: 826 1045 2292 2530 3665 4346 4537 826 Size: 219 1247 238 1135 681 191 1471 Fragments arranged by size: 1471 1247 1135 681 238 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5182 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5182 SmaI CCC'GGG Cuts at: 1872 2509 1872 Size: 637 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3286 4187 4449 820 Size: 197 926 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2973 493 Size: 2480 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5182 SphI G_CATG'C Cuts at: 1132 2540 1132 Size: 1408 3774 SspI AAT'ATT Cuts at: 5 52 3106 5 Size: 47 3054 2081 Fragments arranged by size: 3054 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5182 StyI C'CwwG_G Cuts at: 513 1065 2004 2044 2181 2323 2376 2621 513 Size: 552 939 40 137 142 53 245 3074 Fragments arranged by size: 3074 939 552 245 142 137 53 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2130 3209 3226 3379 3564 4009 325 Size: 1255 550 1079 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1079 550 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3428 5140 96 Size: 274 80 33 51 1443 1451 1712 138 Fragments arranged by size: 1712 1451 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2011 2043 5142 5174 2011 Size: 32 3099 32 2019 Fragments arranged by size: 3099 2019 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5182 XbaI T'CTAG_A Cuts at: 2518 2518 Size: 5182 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5182 XmaI C'CCGG_G Cuts at: 1870 2507 1870 Size: 637 4545 XmnI GAAnn'nnTTC Cuts at: 3311 3311 Size: 5182 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2990 276 Size: 53 83 186 936 1456 2468 Fragments arranged by size: 2468 1456 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI