(Circular) (Six-base) MAPSORT of: pFN21AB8864.seq Check: 564 from: 1 to: 5035 pFN21AB8864 5035 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2179 2188 2179 Size: 9 5026 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2845 278 Size: 53 83 186 936 1309 2468 Fragments arranged by size: 2468 1309 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2378 1937 Size: 441 4594 Acc65I G'GTAC_C Cuts at: 2356 2356 Size: 5035 AclI AA'CG_TT Cuts at: 1563 2334 3162 3535 1563 Size: 771 828 373 3063 Fragments arranged by size: 3063 828 771 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3098 4482 4750 1632 Size: 1466 1384 268 1917 Fragments arranged by size: 1917 1466 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4838 AflIII A'CryG_T Cuts at: 1203 3934 4911 1203 Size: 2731 977 1327 Fragments arranged by size: 2731 1327 977 AgeI A'CCGG_T Cuts at: 3866 3866 Size: 5035 AhdI GACnn_n'nnGTC Cuts at: 3764 3764 Size: 5035 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5035 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5035 AlwNI CAG_nnn'CTG Cuts at: 2301 2778 4350 2301 Size: 477 1572 2986 Fragments arranged by size: 2986 1572 477 ApaI G_GGCC'C Cuts at: 2219 2219 Size: 5035 ApaLI G'TGCA_C Cuts at: 3092 4248 3092 Size: 1156 3879 ApoI r'AATT_y Cuts at: 1493 2344 2570 2722 4831 1493 Size: 851 226 152 2109 1697 Fragments arranged by size: 2109 1697 851 226 152 AseI AT'TA_AT Cuts at: 160 1032 3589 160 Size: 872 2557 1606 Fragments arranged by size: 2557 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5035 AvaI C'yCGr_G Cuts at: 1870 1943 2360 1870 Size: 73 417 4545 Fragments arranged by size: 4545 417 73 BamHI G'GATC_C Cuts at: 2365 2732 2365 Size: 367 4668 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 3811 618 Size: 325 217 627 569 1455 1842 Fragments arranged by size: 1842 1455 627 569 325 217 BanII G_rGCy'C Cuts at: 1391 2219 2354 1391 Size: 828 135 4072 Fragments arranged by size: 4072 828 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4064 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3226 3260 1903 Size: 34 1289 34 3678 Fragments arranged by size: 3678 1289 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2927 4137 2927 Size: 1210 3825 BclI T'GATC_A Cuts at: 1548 1548 Size: 5035 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3646 136 Size: 107 122 71 631 605 1974 1525 Fragments arranged by size: 1974 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5030 5030 Size: 5035 BlpI GC'TnA_GC Cuts at: 2452 2452 Size: 5035 Bme1580I G_kGCm'C Cuts at: 1790 2219 3096 4252 1790 Size: 429 877 1156 2573 Fragments arranged by size: 2573 1156 877 429 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5035 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2766 3724 442 Size: 416 53 83 399 24 1349 958 1753 Fragments arranged by size: 1753 1349 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5035 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2339 3695 1239 Size: 1031 69 1356 2579 Fragments arranged by size: 2579 1356 1031 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3160 4025 4323 3160 Size: 865 298 3872 Fragments arranged by size: 3872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3698 882 Size: 617 2199 2219 Fragments arranged by size: 2219 2199 617 BsaAI yAC'GTr Cuts at: 493 1204 2826 493 Size: 711 1622 2702 Fragments arranged by size: 2702 1622 711 BsaBI GATnn'nnATC Cuts at: 5029 5029 Size: 5035 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2842 3224 3859 275 Size: 53 83 186 936 1309 382 635 1451 Fragments arranged by size: 1451 1309 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3468 3866 4140 4287 4698 3468 Size: 398 274 147 411 3805 Fragments arranged by size: 3805 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4810 Fragments arranged by size: 4810 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5035 BseYI C'CCAG_C Cuts at: 2065 2206 4238 4734 2065 Size: 141 2032 496 2366 Fragments arranged by size: 2366 2032 496 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2244 1163 Size: 573 508 3954 Fragments arranged by size: 3954 573 508 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2485 3246 4274 1540 Size: 275 186 484 761 1028 2301 Fragments arranged by size: 2301 1028 761 484 275 186 BsiHKAI G_wGCw'C Cuts at: 3096 3181 4252 3096 Size: 85 1071 3879 Fragments arranged by size: 3879 1071 85 BsmI GAATG_Cn' Cuts at: 2546 2639 2546 Size: 93 4942 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2354 3096 3181 4252 1391 Size: 399 429 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 135 85 BspEI T'CCGG_A Cuts at: 4698 4698 Size: 5035 BspHI T'CATG_A Cuts at: 2924 4654 2924 Size: 1730 3305 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2179 2188 2193 2390 844 Size: 1335 9 5 197 3489 Fragments arranged by size: 3489 1335 197 9 5 BsrBI CCG'CTC Cuts at: 1394 1823 2922 1394 Size: 429 1099 3507 Fragments arranged by size: 3507 1099 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3530 3704 65 Size: 1183 2282 174 1396 Fragments arranged by size: 2282 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3679 3866 1700 Size: 110 1869 187 2869 Fragments arranged by size: 2869 1869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5035 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5035 BssSI C'ACGA_G Cuts at: 3095 4107 3095 Size: 1012 4023 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5035 BstBI TT'CG_AA Cuts at: 3839 3839 Size: 5035 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5035 BstYI r'GATC_y Cuts at: 1972 2365 2732 3117 3134 4586 4672 4684 5030 Size: 393 367 385 17 1452 86 12 346 Cuts at: 5030 1972 Size: 1977 Fragments arranged by size: 1977 1452 393 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3544 Fragments arranged by size: 3544 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4296 Fragments arranged by size: 4296 683 56 BtsI GCAGTG_nn' Cuts at: 2551 3345 3365 2551 Size: 794 20 4221 Fragments arranged by size: 4221 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5035 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5000 DraI TTT'AAA Cuts at: 2339 2339 Size: 5035 DraIII CAC_nnn'GTG Cuts at: 2110 2110 Size: 5035 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4042 809 Size: 507 2726 1802 Fragments arranged by size: 2726 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 3371 5000 Size: 54 1425 50 275 18 652 889 1629 Cuts at: 5000 8 Size: 43 Fragments arranged by size: 1629 1425 889 652 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2482 1537 Size: 275 670 4090 Fragments arranged by size: 4090 670 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2965 1353 Size: 1612 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3622 3996 4142 387 Size: 1064 138 2033 374 146 1280 Fragments arranged by size: 2033 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2339 3098 3695 4482 4750 1239 Size: 393 638 69 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 638 597 393 268 69 EcoNI CCTnn'n_nnAGG Cuts at: 2223 2223 Size: 5035 EcoO109I rG'GnC_Cy Cuts at: 2057 2182 2215 2216 2256 2057 Size: 125 33 1 40 4836 Fragments arranged by size: 4836 125 40 33 1 EcoRI G'AATT_C Cuts at: 2344 2722 2344 Size: 378 4657 FspI TGC'GCA Cuts at: 3541 3541 Size: 5035 HaeII r_GCGC'y Cuts at: 1678 2042 4182 1678 Size: 364 2140 2531 Fragments arranged by size: 2531 2140 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3676 3708 3750 3782 1373 Size: 32 135 32 2104 32 42 32 2626 Fragments arranged by size: 2626 2104 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2379 2625 669 Size: 988 281 441 246 3079 Fragments arranged by size: 3079 988 441 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5035 HpaI GTT'AAC Cuts at: 2625 2625 Size: 5035 KpnI G_GTAC'C Cuts at: 2360 2360 Size: 5035 MfeI C'AATT_G Cuts at: 2634 2634 Size: 5035 MluI A'CGCG_T Cuts at: 4911 4911 Size: 5035 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4149 4333 1305 Size: 2844 184 2007 Fragments arranged by size: 2844 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3213 Fragments arranged by size: 3213 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2993 3352 3511 518 Size: 648 1827 359 159 2042 Fragments arranged by size: 2042 1827 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2317 2451 3128 4276 4521 1606 Size: 711 134 677 1148 245 2120 Fragments arranged by size: 2120 1148 711 677 245 134 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3544 Fragments arranged by size: 3544 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5035 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5035 NotI GC'GGCC_GC Cuts at: 2482 2482 Size: 5035 NspI r_CATG'y Cuts at: 1132 1229 2393 3938 1132 Size: 97 1164 1545 2229 Fragments arranged by size: 2229 1545 1164 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5035 PciI A'CATG_T Cuts at: 3934 3934 Size: 5035 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5035 PmeI GTTT'AAAC Cuts at: 2339 2339 Size: 5035 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 3136 4617 4649 3104 Size: 32 1481 32 3490 Fragments arranged by size: 3490 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2182 2256 2057 Size: 125 74 4836 Fragments arranged by size: 4836 125 74 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5035 PsiI TTA'TAA Cuts at: 2605 2605 Size: 5035 PspOMI G'GGCC_C Cuts at: 2215 2215 Size: 5035 PstI C_TGCA'G Cuts at: 830 2190 2238 2265 2387 830 Size: 1360 48 27 122 3478 Fragments arranged by size: 3478 1360 122 48 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5035 PvuII CAG'CTG Cuts at: 2317 2317 Size: 5035 SalI G'TCGA_C Cuts at: 1936 2377 1936 Size: 441 4594 SanDI GG'GwC_CC Cuts at: 2256 2256 Size: 5035 SbfI CC_TGCA'GG Cuts at: 2190 2387 2190 Size: 197 4838 ScaI AGT'ACT Cuts at: 3283 4995 3283 Size: 1712 3323 SexAI A'CCwGG_T Cuts at: 2106 2106 Size: 5035 SfcI C'TryA_G Cuts at: 826 1045 2186 2234 2261 2383 3518 4199 4390 Size: 219 1141 48 27 122 1135 681 191 Cuts at: 4390 826 Size: 1471 Fragments arranged by size: 1471 1141 1135 681 219 191 122 48 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5035 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5035 SmaI CCC'GGG Cuts at: 1872 2362 1872 Size: 490 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3139 4040 4302 820 Size: 197 926 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2826 493 Size: 2333 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5035 SphI G_CATG'C Cuts at: 1132 2393 1132 Size: 1261 3774 SspI AAT'ATT Cuts at: 5 52 2959 5 Size: 47 2907 2081 Fragments arranged by size: 2907 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5035 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2474 513 Size: 552 939 237 233 3074 Fragments arranged by size: 3074 939 552 237 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 3062 3079 3232 3417 3862 325 Size: 1255 593 889 17 153 185 445 1498 Fragments arranged by size: 1498 1255 889 593 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3281 4993 96 Size: 274 80 33 51 1443 1304 1712 138 Fragments arranged by size: 1712 1443 1304 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4995 5027 4995 Size: 32 5003 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5035 XbaI T'CTAG_A Cuts at: 2371 2371 Size: 5035 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5035 XmaI C'CCGG_G Cuts at: 1870 2360 1870 Size: 490 4545 XmnI GAAnn'nnTTC Cuts at: 3164 3164 Size: 5035 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2843 276 Size: 53 83 186 936 1309 2468 Fragments arranged by size: 2468 1309 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI