################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MaeI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1150 GACGTC GACGT^C AlwNI 403 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1342 GGGCCC GGGCC^C Asp700I 115 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 1555 GGCGCC GGCGC^C BbrPI 520 CACGTG CAC^GTG BclI 776 TGATCA T^GATCA BglI 1435 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1527 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1527 GCTCAGC GC^T[ACGT]AGC BsaAI 520 CACGTG [CT]AC^GT[GA] BsgI 232 GTGCAG GTGCAG(16/14) BsmBI 130 CGTCTC CGTCTC(1/5) BsmI 1722 GCATTC GAATGC(1/-1) BspLU11I 1415 ACATGT A^CATGT BspMI 922 ACCTGC ACCTGC(4/8) BstEII 460 GGTGACC G^GT[ACGT]ACC BstPI 460 GGTGACC G^GT[ACGT]ACC CelII 1527 GCTCAGC GC^T[ACGT]AGC DraIII 797 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 904 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 530 CGGCCA [CT]^GGCC[GA] EcoO65I 460 GGTGACC G^GT[ACGT]ACC FbaI 776 TGATCA T^GATCA HaeII 1555 GGCGCC [GA]GCGC^[CT] HinfI 150 GAGTC G^A[ACGT]TC KasI 1555 GGCGCC G^GCGCC KspI 1662 CCGCGG CCGC^GG MseI 719 TTAA T^TAA NarI 1555 GGCGCC GG^CGCC NcoI 1373 CCATGG C^CATGG NspI 1415 ACATGT [GA]CATG^[CT] PleI 150 GAGTC GAGTC(4/5) PmaCI 520 CACGTG CAC^GTG PmlI 520 CACGTG CAC^GTG SacII 1662 CCGCGG CCGC^GG SapI 1473 GCTCTTC GCTCTTC(1/4) SfoI 1555 GGCGCC GGC^GCC SgrAI 1551 CACCGGCG C[GA]^CCGG[CT]G SmaI 58 CCCGGG CCC^GGG StuI 1613 AGGCCT AGG^CCT Tru9I 719 TTAA T^TAA XcmI 1247 CCACACTGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 58 CCCGGG C^CCGGG XmaI 58 CCCGGG C^CCGGG XmnI 115 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 670 AAATTC [GA]^AATT[CT] 1045 GAATTT AcyI 1150 GACGTC G[GA]^CG[CT]C 1555 GGCGCC AflIII 422 ACGTGT A^C[GA][CT]GT 1415 ACATGT ApoI 670 AAATTC [GA]^AATT[CT] 1045 GAATTT AspHI 1087 GTGCTC G[AT]GC[AT]^C 1525 GTGCTC BamHI 585 GGATCC G^GATCC 809 GGATCC BanII 250 GGGCTC G[GA]GC[CT]^C 1342 GGGCCC BsaBI 810 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1116 GATCGGCATC BsaHI 1150 GACGTC G[GA]^CG[CT]C 1555 GGCGCC BsaI 31 GAGACC GGTCTC(1/5) 826 GAGACC BsiHKAI 1087 GTGCTC G[AT]GC[AT]^C 1525 GTGCTC BspHI 146 TCATGA T^CATGA 1109 TCATGA BstAPI 182 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1722 GCATTCGCTGC BstXI 815 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1130 CCAAGAACTTGG Hin1I 1150 GACGTC G[GA]^CG[CT]C 1555 GGCGCC MamI 810 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1116 GATCGGCATC PpuMI 67 GGGACCC [GA]G^G[AT]CC[CT] 1706 GGGACCC PvuII 262 CAGCTG CAG^CTG 370 CAGCTG RcaI 146 TCATGA T^CATGA 1109 TCATGA Tsp509I 671 AATT ^AATT 1046 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI AvaII BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII