################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BciVI BclI BglI BglII BlnI BmyI BpmI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 111 GAATTT [GA]^AATT[CT] AfaI 303 GTAC GT^AC AflII 286 CTTAAG C^TTAAG ApoI 111 GAATTT [GA]^AATT[CT] AvaI 347 CCCGGG C^[CT]CG[GA]G BamHI 483 GGATCC G^GATCC BcgI 596 GCACCACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 577 CTAG C^TAG BfrI 286 CTTAAG C^TTAAG BsgI 594 CTGCAC GTGCAG(16/14) BsoBI 347 CCCGGG C^[CT]CG[GA]G BspMI 592 ACCTGC ACCTGC(4/8) BsrDI 716 GCAATG GCAATG(2/0) CfoI 638 GCGC GCG^C DraIII 475 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 566 GACTGGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 390 GAAGAG CTCTTC(1/4) HaeII 637 GGCGCT [GA]GCGC^[CT] HhaI 638 GCGC GCG^C HinP1I 638 GCGC G^CGC HincII 418 GTTAAC GT[CT]^[GA]AC HindII 418 GTTAAC GT[CT]^[GA]AC HinfI 84 GAATC G^A[ACGT]TC HpaI 418 GTTAAC GTT^AAC Ksp632I 390 GAAGAG CTCTTC(1/4) MaeI 577 CTAG C^TAG PflMI 252 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 303 GTAC GT^AC SmaI 347 CCCGGG CCC^GGG SmlI 286 CTTAAG C^T[CT][GA]AG StuI 103 AGGCCT AGG^CCT TfiI 84 GAATC G^A[AT]TC Van91I 252 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 347 CCCGGG C^CCGGG XmaI 347 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AatII 379 GACGTC GACGT^C 572 GACGTC AcyI 379 GACGTC G[GA]^CG[CT]C 572 GACGTC AhdI 395 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 572 GACGTCTAGTC AlwNI 253 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 334 CAGAGACTG AspEI 395 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 572 GACGTCTAGTC BcnI 347 CCCGG CC^[GC]GG 348 CCGGG BlpI 454 GCTCAGC GC^T[ACGT]AGC 646 GCTAAGC Bpu1102I 454 GCTCAGC GC^T[ACGT]AGC 646 GCTAAGC BsaHI 379 GACGTC G[GA]^CG[CT]C 572 GACGTC BsaI 146 GAGACC GGTCTC(1/5) 393 GAGACC BsiYI 252 CCAGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 702 CCGTCACTGGG BslI 252 CCAGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 702 CCGTCACTGGG BstF5I 132 CATCC GGATG(2/0) 204 CATCC BstYI 483 GGATCC [GA]^GATC[CT] 695 AGATCC CelII 454 GCTCAGC GC^T[ACGT]AGC 646 GCTAAGC DpnII 484 GATC ^GATC 696 GATC Eam1105I 395 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 572 GACGTCTAGTC EcoT14I 20 CCAAGG C^C[AT][AT]GG 434 CCTTGG FauI 155 CCCGC CCCGC(4/6) 173 CCCGC FokI 132 CATCC GGATG(9/13) 204 CATCC HapII 40 CCGG C^CGG 348 CCGG Hin1I 379 GACGTC G[GA]^CG[CT]C 572 GACGTC HpaII 40 CCGG C^CGG 348 CCGG MaeIII 427 GTCAC ^GT[ACGT]AC 704 GTCAC MboI 484 GATC ^GATC 696 GATC MflI 483 GGATCC [GA]^GATC[CT] 695 AGATCC MspI 40 CCGG C^CGG 348 CCGG NciI 347 CCCGG CC^[GC]GG 348 CCGGG NdeII 484 GATC ^GATC 696 GATC PpuMI 533 GGGTCCC [GA]G^G[AT]CC[CT] 726 GGGTCCT Sau3AI 484 GATC ^GATC 696 GATC StyI 20 CCAAGG C^C[AT][AT]GG 434 CCTTGG Tsp45I 427 GTCAC ^GT[GC]AC 704 GTCAC Tsp509I 112 AATT ^AATT 673 AATT TspRI 600 CACTG CA[GC]TG[ACGT][ACGT]^ 706 CACTG XhoII 483 GGATCC [GA]^GATC[CT] 695 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI AlwI AvaII BbsI BbvI BpuAI BsaJI BsmAI BsmFI BsrI BssKI BstNI BstXI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI MaeII MboII MnlI MseI MslI MspA1I MvaI MwoI NlaIV PvuII Sau96I ScrFI TaqI Tru9I TseI TthHB8I