(Linear) (Six-base) MAPSORT of: KIEE5304.seq Check: 5722 from: 1 to: 798 KIEE5304.orf With 182 enzymes: SgfI * January 29, 2014 11:54 .. AatII G_ACGT'C Cuts at: 0 383 576 798 Size: 383 193 222 Fragments arranged by size: 383 222 193 AflII C'TTAA_G Cuts at: 0 286 798 Size: 286 512 AhdI GACnn_n'nnGTC Cuts at: 0 400 577 798 Size: 400 177 221 Fragments arranged by size: 400 221 177 AleI CACnn'nnGTG Cuts at: 0 436 616 710 798 Size: 436 180 94 88 Fragments arranged by size: 436 180 94 88 AlwNI CAG_nnn'CTG Cuts at: 0 258 339 798 Size: 258 81 459 Fragments arranged by size: 459 258 81 ApoI r'AATT_y Cuts at: 0 111 798 Size: 111 687 AvaI C'yCGr_G Cuts at: 0 347 798 Size: 347 451 BamHI G'GATC_C Cuts at: 0 483 798 Size: 483 315 BbsI GAAGACnn'nnnn_ Cuts at: 0 495 595 635 798 Size: 495 100 40 163 Fragments arranged by size: 495 163 100 40 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 585 619 798 Size: 585 34 179 Fragments arranged by size: 585 179 34 BlpI GC'TnA_GC Cuts at: 0 455 647 798 Size: 455 192 151 Fragments arranged by size: 455 192 151 BmgBI CAC'GTC Cuts at: 0 480 798 Size: 480 318 BmrI ACTGGGnnnn_n' Cuts at: 0 577 717 798 Size: 577 140 81 Fragments arranged by size: 577 140 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 223 798 Size: 223 575 BsaI GGTCTCn'nnnn_ Cuts at: 0 140 387 798 Size: 140 247 411 Fragments arranged by size: 411 247 140 BsaHI Gr'CG_yC Cuts at: 0 380 573 798 Size: 380 193 225 Fragments arranged by size: 380 225 193 BseYI C'CCAG_C Cuts at: 0 45 798 Size: 45 753 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 579 798 Size: 579 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 601 798 Size: 601 197 BsrDI GCAATG_nn' Cuts at: 0 723 798 Size: 723 75 BstXI CCAn_nnnn'nTGG Cuts at: 0 438 495 792 798 Size: 438 57 297 6 Fragments arranged by size: 438 297 57 6 BstYI r'GATC_y Cuts at: 0 483 695 798 Size: 483 212 103 Fragments arranged by size: 483 212 103 DraIII CAC_nnn'GTG Cuts at: 0 480 798 Size: 480 318 DrdI GACnn_nn'nnGTC Cuts at: 0 572 798 Size: 572 226 EarI CTCTTCn'nnn_ Cuts at: 0 385 798 Size: 385 413 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 688 798 Size: 688 110 EcoO109I rG'GnC_Cy Cuts at: 0 255 534 727 798 Size: 255 279 193 71 Fragments arranged by size: 279 255 193 71 HaeII r_GCGC'y Cuts at: 0 641 798 Size: 641 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 116 148 386 418 563 595 687 719 Size: 116 32 238 32 145 32 92 32 Cuts at: 719 798 Size: 79 Fragments arranged by size: 238 145 116 92 79 32 32 32 32 HincII GTy'rAC Cuts at: 0 420 798 Size: 420 378 HpaI GTT'AAC Cuts at: 0 420 798 Size: 420 378 MslI CAynn'nnrTG Cuts at: 0 436 616 710 798 Size: 436 180 94 88 Fragments arranged by size: 436 180 94 88 MspA1I CmG'CkG Cuts at: 0 49 192 282 459 798 Size: 49 143 90 177 339 Fragments arranged by size: 339 177 143 90 49 PflMI CCAn_nnn'nTGG Cuts at: 0 258 798 Size: 258 540 PpuMI rG'GwC_Cy Cuts at: 0 534 727 798 Size: 534 193 71 Fragments arranged by size: 534 193 71 PvuII CAG'CTG Cuts at: 0 49 282 459 798 Size: 49 233 177 339 Fragments arranged by size: 339 233 177 49 SanDI GG'GwC_CC Cuts at: 0 534 798 Size: 534 264 SmaI CCC'GGG Cuts at: 0 349 798 Size: 349 449 SmlI C'TyrA_G Cuts at: 0 286 798 Size: 286 512 SrfI GCCC'GGGC Cuts at: 0 349 798 Size: 349 449 StuI AGG'CCT Cuts at: 0 105 798 Size: 105 693 StyI C'CwwG_G Cuts at: 0 20 434 798 Size: 20 414 364 Fragments arranged by size: 414 364 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 265 798 Size: 265 533 TatI w'GTAC_w Cuts at: 0 302 798 Size: 302 496 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 17 49 798 Size: 17 32 749 Fragments arranged by size: 749 32 17 XmaI C'CCGG_G Cuts at: 0 347 798 Size: 347 451 ZraI GAC'GTC Cuts at: 0 381 574 798 Size: 381 193 224 Fragments arranged by size: 381 224 193 Enzymes that do cut: AatII AflII AhdI AleI AlwNI ApoI AvaI BamHI BbsI BcgI BlpI BmgBI BmrI BplI BsaI BsaHI BseYI BsgI BspMI BsrDI BstXI BstYI DraIII DrdI EarI EciI EcoO109I HaeII Hin4I HincII HpaI MslI MspA1I PflMI PpuMI PvuII SanDI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EaeI EagI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XcmI XhoI XmnI