################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRII EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MscI MslI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TfiI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 228 TCCGGA T^CCGGA AciI 5 CCGC CCGC(-3/-1) AcsI 101 GAATTC [GA]^AATT[CT] AfaI 212 GTAC GT^AC AlwI 146 GGATC GGATC(4/5) ApoI 101 GAATTC [GA]^AATT[CT] Asp700I 241 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 123 GAAGAC GAAGAC(2/6) BcgI 30 CGACCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 175 CTAG C^TAG BpuAI 123 GAAGAC GAAGAC(2/6) BsaJI 141 CCAAGG C^C[ACGT][ACGT]GG BsaWI 228 TCCGGA [AT]^CCGG[AT] BseAI 228 TCCGGA T^CCGGA BsiYI 229 CCGGAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 229 CCGGAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspEI 228 TCCGGA T^CCGGA BspMI 62 ACCTGC ACCTGC(4/8) BsrDI 206 GCAATG GCAATG(2/0) BstEII 259 GGTGACC G^GT[ACGT]ACC BstF5I 226 CATCC GGATG(2/0) BstPI 259 GGTGACC G^GT[ACGT]ACC EcoO65I 259 GGTGACC G^GT[ACGT]ACC EcoRI 101 GAATTC G^AATTC EcoT14I 141 CCAAGG C^C[AT][AT]GG FokI 226 CATCC GGATG(9/13) HapII 229 CCGG C^CGG HpaII 229 CCGG C^CGG MaeI 175 CTAG C^TAG MaeIII 260 GTGAC ^GT[ACGT]AC MboII 123 GAAGA GAAGA(8/7) MroI 228 TCCGGA T^CCGGA MseI 188 TTAA T^TAA MspA1I 319 CAGCTG C[AC]G^C[GT]G MspI 229 CCGG C^CGG NdeI 276 CATATG CA^TATG NlaIII 331 CATG CATG^ PvuII 319 CAGCTG CAG^CTG RsaI 212 GTAC GT^AC SfcI 253 CTACAG C^T[GA][CT]AG SpeI 174 ACTAGT A^CTAGT StyI 141 CCAAGG C^C[AT][AT]GG TaqI 29 TCGA T^CGA Tru9I 188 TTAA T^TAA Tsp45I 260 GTGAC ^GT[GC]AC TspRI 78 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 29 TCGA T^CGA XmnI 241 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 251 AGCT AG^CT 320 AGCT BsrI 303 ACTGG ACTGG(1/-1) 282 CCAGT Cac8I 73 GCTGGC GC[ACGT]^[ACGT]GC 202 GCCTGC DdeI 80 CTGAG C^T[ACGT]AG 308 CTAAG DpnII 147 GATC ^GATC 316 GATC HphI 55 GGTGA GGTGA(8/7) 259 GGTGA MboI 147 GATC ^GATC 316 GATC NdeII 147 GATC ^GATC 316 GATC Sau3AI 147 GATC ^GATC 316 GATC SfaNI 43 GATGC GCATC(5/9) 225 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MnlI Tsp509I