Restriction map for pFN21AE5111
 (Circular) (Six-base) MAPSORT of: pFN21AE5111.seq  Check: 2489  from: 1  to: 5044



pFN21AE5111  5044 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:12  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2854     278 
   Size:         53      83     186     936    1318    2468

  Fragments arranged by size: 

                2468    1318     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2387    1937 
   Size:        450    4594
 
Acc65I G'GTAC_C

Cuts at:   2365    2365 
   Size:       5044
 
AclI AA'CG_TT

Cuts at:   1563    3171    3544    1563 
   Size:       1608     373    3063

  Fragments arranged by size: 

                3063    1608     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2348    3107    4491    4759    1632 
   Size:        716     759    1384     268    1917

  Fragments arranged by size: 

                1917    1384     759     716     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4847
 
AflIII A'CryG_T

Cuts at:   1203    3943    4920    1203 
   Size:       2740     977    1327

  Fragments arranged by size: 

                2740    1327     977
 
AgeI A'CCGG_T

Cuts at:   3875    3875 
   Size:       5044
 
AhdI GACnn_n'nnGTC

Cuts at:   3773    3773 
   Size:       5044
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5044
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5044
 
AlwNI CAG_nnn'CTG

Cuts at:   2787    4359    2787 
   Size:       1572    3472
 
ApaLI G'TGCA_C

Cuts at:   3101    4257    3101 
   Size:       1156    3888
 
ApoI r'AATT_y

Cuts at:   1493    2106    2353    2579    2731    4840    1493 
   Size:        613     247     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     613     247     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3598     160 
   Size:        872    2566    1606

  Fragments arranged by size: 

                2566    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5044
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2369    1870 
   Size:         73     426    4545

  Fragments arranged by size: 

                4545     426      73
 
BamHI G'GATC_C

Cuts at:   2374    2741    2374 
   Size:        367    4677
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2365    3820     618 
   Size:        325     217     627     578    1455    1842

  Fragments arranged by size: 

                1842    1455     627     578     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2363    1391 
   Size:        972    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2135     928 
   Size:        971     236    3837

  Fragments arranged by size: 

                3837     971     236
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2024    2058    3235    3269    1903 
   Size:         34      87      34    1177      34    3678

  Fragments arranged by size: 

                3678    1177      87      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2936    4146    2936 
   Size:       1210    3834
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5044
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3655     136 
   Size:        107     122      71     631     605    1983    1525

  Fragments arranged by size: 

                1983    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5039    5039 
   Size:       5044
 
BlpI GC'TnA_GC

Cuts at:   2461    2461 
   Size:       5044
 
Bme1580I G_kGCm'C

Cuts at:   1790    3105    4261    1790 
   Size:       1315    1156    2573

  Fragments arranged by size: 

                2573    1315    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5044
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2775    3733     442 
   Size:        416      53      83     399      24    1358     958    1753

  Fragments arranged by size: 

                1753    1358     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5044
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3704    1239 
   Size:       2465    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2313    2313 
   Size:       5044
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3169    4034    4332    3169 
   Size:        865     298    3881

  Fragments arranged by size: 

                3881     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3707     882 
   Size:        617    2208    2219

  Fragments arranged by size: 

                2219    2208     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2835     493 
   Size:        711    1631    2702

  Fragments arranged by size: 

                2702    1631     711
 
BsaBI GATnn'nnATC

Cuts at:   5038    5038 
   Size:       5044
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2851    3233    3868     275 
   Size:         53      83     186     936    1318     382     635    1451

  Fragments arranged by size: 

                1451    1318     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2233    3477    3875    4149    4296    4707    2233 
   Size:       1244     398     274     147     411    2570

  Fragments arranged by size: 

                2570    1244     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4819

  Fragments arranged by size: 

                4819     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5044
 
BseYI C'CCAG_C

Cuts at:   4247    4743    4247 
   Size:        496    4548
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4471
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2494    3255    4283    1540 
   Size:        275     186     493     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     493     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3105    3190    4261    3105 
   Size:         85    1071    3888

  Fragments arranged by size: 

                3888    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2555    2648    2555 
   Size:         93    4951
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2363    3105    3190    4261    1391 
   Size:        399     573     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     573     399      85
 
BspEI T'CCGG_A

Cuts at:   2233    4707    2233 
   Size:       2474    2570
 
BspHI T'CATG_A

Cuts at:   2933    4663    2933 
   Size:       1730    3314
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2076    2399     844 
   Size:       1232     323    3489

  Fragments arranged by size: 

                3489    1232     323
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2931    1394 
   Size:        429    1108    3507

  Fragments arranged by size: 

                3507    1108     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2218    3539    3713      65 
   Size:       1183     970    1321     174    1396

  Fragments arranged by size: 

                1396    1321    1183     970     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3688    3875    1700 
   Size:        110    1878     187    2869

  Fragments arranged by size: 

                2869    1878     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5044
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5044
 
BssSI C'ACGA_G

Cuts at:   3104    4116    3104 
   Size:       1012    4032
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5044
 
BstBI TT'CG_AA

Cuts at:   3848    3848 
   Size:       5044
 
BstEII G'GTnAC_C

Cuts at:   1184    2264    1184 
   Size:       1080    3964
 
BstYI r'GATC_y

Cuts at:   1972    2374    2741    3126    3143    4595    4681    4693    5039 
   Size:        402     367     385      17    1452      86      12     346

Cuts at:   5039    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     402     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4492
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4305

  Fragments arranged by size: 

                4305     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2560    3354    3374    2560 
   Size:        794      20    4230

  Fragments arranged by size: 

                4230     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5044
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5009
 
DraI TTT'AAA

Cuts at:   2348    2348 
   Size:       5044
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4051     809 
   Size:        507    2735    1802

  Fragments arranged by size: 

                2735    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2491    3380    5009 
   Size:         54    1425      50     275      18     661     889    1629

Cuts at:   5009       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     661     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2491    1537 
   Size:        275     679    4090

  Fragments arranged by size: 

                4090     679     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2974    1353 
   Size:       1621    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3631    4005    4151     387 
   Size:       1064     138    2042     374     146    1280

  Fragments arranged by size: 

                2042    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2348    3107    3704    4491    4759    1239 
   Size:        393     716     759     597     787     268    1524

  Fragments arranged by size: 

                1524     787     759     716     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2106    2353    2731    2106 
   Size:        247     378    4419

  Fragments arranged by size: 

                4419     378     247
 
FspI TGC'GCA

Cuts at:   3550    3550 
   Size:       5044
 
HaeII r_GCGC'y

Cuts at:   1678    4191    1678 
   Size:       2513    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3685    3717    3759    3791    1373 
   Size:         32     135      32    2113      32      42      32    2626

  Fragments arranged by size: 

                2626    2113     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2388    2634     669 
   Size:        988     281     450     246    3079

  Fragments arranged by size: 

                3079     988     450     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5044
 
HpaI GTT'AAC

Cuts at:   2634    2634 
   Size:       5044
 
KpnI G_GTAC'C

Cuts at:   2369    2369 
   Size:       5044
 
MfeI C'AATT_G

Cuts at:   2643    2643 
   Size:       5044
 
MluI A'CGCG_T

Cuts at:   4920    4920 
   Size:       5044
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4158    4342    1305 
   Size:       2853     184    2007

  Fragments arranged by size: 

                2853    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3222

  Fragments arranged by size: 

                3222    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3002    3361    3520     518 
   Size:        648    1836     359     159    2042

  Fragments arranged by size: 

                2042    1836     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2326    2460    3137    4285    4530    1606 
   Size:        720     134     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     720     677     245     134
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4492
 
NdeI CA'TA_TG

Cuts at:    387    2282     387 
   Size:       1895    3149
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5044
 
NotI GC'GGCC_GC

Cuts at:   2491    2491 
   Size:       5044
 
NspI r_CATG'y

Cuts at:   1132    1229    2402    3947    1132 
   Size:         97    1173    1545    2229

  Fragments arranged by size: 

                2229    1545    1173      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5044
 
PciI A'CATG_T

Cuts at:   3943    3943 
   Size:       5044
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5044
 
PmeI GTTT'AAAC

Cuts at:   2348    2348 
   Size:       5044
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2012    2044    3113    3145    4626    4658    2012 
   Size:         32    1069      32    1481      32    2398

  Fragments arranged by size: 

                2398    1481    1069      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5044
 
PsiI TTA'TAA

Cuts at:   2614    2614 
   Size:       5044
 
PstI C_TGCA'G

Cuts at:    830    2396     830 
   Size:       1566    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5044
 
PvuII CAG'CTG

Cuts at:   2326    2326 
   Size:       5044
 
SalI G'TCGA_C

Cuts at:   1936    2386    1936 
   Size:        450    4594
 
SbfI CC_TGCA'GG

Cuts at:   2396    2396 
   Size:       5044
 
ScaI AGT'ACT

Cuts at:   3292    5004    3292 
   Size:       1712    3332
 
SfcI C'TryA_G

Cuts at:    826    1045    2258    2392    3527    4208    4399     826 
   Size:        219    1213     134    1135     681     191    1471

  Fragments arranged by size: 

                1471    1213    1135     681     219     191     134
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5044
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5044
 
SmaI CCC'GGG

Cuts at:   1872    2371    1872 
   Size:        499    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3148    4049    4311     820 
   Size:        197     926    1205     901     262    1553

  Fragments arranged by size: 

                1553    1205     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2835     493 
   Size:       2342    2702
 
SpeI A'CTAG_T

Cuts at:    152    2179     152 
   Size:       2027    3017
 
SphI G_CATG'C

Cuts at:   1132    2402    1132 
   Size:       1270    3774
 
SspI AAT'ATT

Cuts at:      5      52    2968       5 
   Size:         47    2916    2081

  Fragments arranged by size: 

                2916    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5044
 
StyI C'CwwG_G

Cuts at:    513    1065    2146    2483     513 
   Size:        552    1081     337    3074

  Fragments arranged by size: 

                3074    1081     552     337
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3071    3088    3241    3426    3871     325 
   Size:       1255    1491      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1491    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3290    5002      96 
   Size:        274      80      33      51    1443    1313    1712     138

  Fragments arranged by size: 

                1712    1443    1313     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5004    5036    5004 
   Size:         32    5012
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5044
 
XbaI T'CTAG_A

Cuts at:   2380    2380 
   Size:       5044
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5044
 
XmaI C'CCGG_G

Cuts at:   1870    2369    1870 
   Size:        499    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2250    3173    2250 
   Size:        923    4121
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2852     276 
   Size:         53      83     186     936    1318    2468

  Fragments arranged by size: 

                2468    1318     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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