(Circular) (Six-base) MAPSORT of: pFN21AB9982.seq Check: 7089 from: 1 to: 6832 pFN21AB9982 6832 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2685 2685 Size: 6832 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2034 4642 278 Size: 53 83 186 936 498 2608 2468 Fragments arranged by size: 2608 2468 936 498 186 83 53 AccI GT'mk_AC Cuts at: 1937 2068 2217 3206 4175 1937 Size: 131 149 989 969 4594 Fragments arranged by size: 4594 989 969 149 131 Acc65I G'GTAC_C Cuts at: 3149 4153 3149 Size: 1004 5828 AclI AA'CG_TT Cuts at: 1563 4959 5332 1563 Size: 3396 373 3063 Fragments arranged by size: 3396 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3196 4089 4895 6279 6547 1632 Size: 1564 893 806 1384 268 1917 Fragments arranged by size: 1917 1564 1384 893 806 268 AfeI AGC'GCT Cuts at: 2894 2894 Size: 6832 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6635 AflIII A'CryG_T Cuts at: 1203 2919 5731 6708 1203 Size: 1716 2812 977 1327 Fragments arranged by size: 2812 1716 1327 977 AgeI A'CCGG_T Cuts at: 5663 5663 Size: 6832 AhdI GACnn_n'nnGTC Cuts at: 4114 5561 4114 Size: 1447 5385 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6832 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6832 AlwNI CAG_nnn'CTG Cuts at: 2626 4575 6147 2626 Size: 1949 1572 3311 Fragments arranged by size: 3311 1949 1572 ApaI G_GGCC'C Cuts at: 3019 3019 Size: 6832 ApaLI G'TGCA_C Cuts at: 2419 3104 4889 6045 2419 Size: 685 1785 1156 3206 Fragments arranged by size: 3206 1785 1156 685 ApoI r'AATT_y Cuts at: 1493 2342 2450 4141 4367 4519 6628 1493 Size: 849 108 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 849 226 152 108 AseI AT'TA_AT Cuts at: 160 1032 5386 160 Size: 872 4354 1606 Fragments arranged by size: 4354 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6832 AvaI C'yCGr_G Cuts at: 1870 1943 4157 1870 Size: 73 2214 4545 Fragments arranged by size: 4545 2214 73 BamHI G'GATC_C Cuts at: 2782 2927 4121 4162 4529 2782 Size: 145 1194 41 367 5085 Fragments arranged by size: 5085 1194 367 145 41 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2163 3149 4153 5608 618 Size: 325 217 627 376 986 1004 1455 1842 Fragments arranged by size: 1842 1455 1004 986 627 376 325 217 BanII G_rGCy'C Cuts at: 1391 3019 3944 4019 4151 1391 Size: 1628 925 75 132 4072 Fragments arranged by size: 4072 1628 925 132 75 BbeI G_GCGC'C Cuts at: 2167 2167 Size: 6832 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3858 928 Size: 971 1959 3902 Fragments arranged by size: 3902 1959 971 BbvCI CC'TCA_GC Cuts at: 2402 3804 2402 Size: 1402 5430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3308 3342 5023 5057 1903 Size: 34 1371 34 1681 34 3678 Fragments arranged by size: 3678 1681 1371 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4724 5934 4724 Size: 1210 5622 BclI T'GATC_A Cuts at: 1548 2772 1548 Size: 1224 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2088 3578 5443 Size: 107 122 71 631 605 416 1490 1865 Cuts at: 5443 136 Size: 1525 Fragments arranged by size: 1865 1525 1490 631 605 416 122 107 71 BglII A'GATC_T Cuts at: 3123 3753 6827 3123 Size: 630 3074 3128 Fragments arranged by size: 3128 3074 630 BlpI GC'TnA_GC Cuts at: 3971 4249 3971 Size: 278 6554 Bme1580I G_kGCm'C Cuts at: 1790 2423 3009 3019 3108 3200 4893 6049 1790 Size: 633 586 10 89 92 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 633 586 92 89 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6832 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2338 2410 3013 Size: 416 53 83 399 24 921 72 603 Cuts at: 3013 4563 5521 442 Size: 1550 958 1753 Fragments arranged by size: 1753 1550 958 921 603 416 399 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6832 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2275 2275 Size: 6832 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 3089 3499 5492 1239 Size: 800 1050 410 1993 2579 Fragments arranged by size: 2579 1993 1050 800 410 Bpu10I CC'TnA_GC Cuts at: 2402 3049 3804 2402 Size: 647 755 5430 Fragments arranged by size: 5430 755 647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2309 3946 4957 5822 6120 2309 Size: 1637 1011 865 298 3021 Fragments arranged by size: 3021 1637 1011 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3179 5495 882 Size: 617 1680 2316 2219 Fragments arranged by size: 2316 2219 1680 617 BsaAI yAC'GTr Cuts at: 493 1204 4623 493 Size: 711 3419 2702 Fragments arranged by size: 3419 2702 711 BsaBI GATnn'nnATC Cuts at: 6826 6826 Size: 6832 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2164 4639 5021 Size: 53 83 186 936 498 133 2475 382 Cuts at: 5021 5656 275 Size: 635 1451 Fragments arranged by size: 2475 1451 936 635 498 382 186 133 83 53 BsaWI w'CCGG_w Cuts at: 5265 5663 5937 6084 6495 5265 Size: 398 274 147 411 5602 Fragments arranged by size: 5602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2606 2636 1178 Size: 30 165 30 1203 30 5374 Fragments arranged by size: 5374 1203 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2622 2819 3245 3410 3479 3497 3502 3800 Size: 1436 197 426 165 69 18 5 298 Cuts at: 3800 4063 4066 4069 1186 Size: 263 3 3 3949 Fragments arranged by size: 3949 1436 426 298 263 197 165 69 18 5 3 3 BseYI C'CCAG_C Cuts at: 2138 4097 6035 6531 2138 Size: 1959 1938 496 2439 Fragments arranged by size: 2439 1959 1938 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 1163 Size: 573 921 5338 Fragments arranged by size: 5338 921 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4282 5043 6071 1540 Size: 275 186 2281 761 1028 2301 Fragments arranged by size: 2301 2281 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2423 3108 4019 4893 4978 6049 2423 Size: 685 911 874 85 1071 3206 Fragments arranged by size: 3206 1071 911 874 685 85 BsmI GAATG_Cn' Cuts at: 3406 4343 4436 3406 Size: 937 93 5802 Fragments arranged by size: 5802 937 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2423 3009 3019 3108 3200 3944 4019 Size: 399 633 586 10 89 92 744 75 Cuts at: 4019 4151 4893 4978 6049 1391 Size: 132 742 85 1071 2174 Fragments arranged by size: 2174 1071 744 742 633 586 399 132 92 89 85 75 10 BspEI T'CCGG_A Cuts at: 6495 6495 Size: 6832 BspHI T'CATG_A Cuts at: 2955 4721 6451 2955 Size: 1766 1730 3336 Fragments arranged by size: 3336 1766 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 4187 844 Size: 1841 1502 3489 Fragments arranged by size: 3489 1841 1502 BsrBI CCG'CTC Cuts at: 1394 1823 3073 4719 1394 Size: 429 1250 1646 3507 Fragments arranged by size: 3507 1646 1250 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2413 5327 5501 65 Size: 1183 1165 2914 174 1396 Fragments arranged by size: 2914 1396 1183 1165 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5476 5663 1700 Size: 110 3666 187 2869 Fragments arranged by size: 3666 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6832 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6832 BssSI C'ACGA_G Cuts at: 2634 3144 4892 5904 2634 Size: 510 1748 1012 3562 Fragments arranged by size: 3562 1748 1012 510 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6832 BstBI TT'CG_AA Cuts at: 5636 5636 Size: 6832 BstEII G'GTnAC_C Cuts at: 1184 2375 1184 Size: 1191 5641 BstXI CCAn_nnnn'nTGG Cuts at: 2522 2522 Size: 6832 BstYI r'GATC_y Cuts at: 1972 2655 2703 2782 2927 3123 3387 3730 3753 Size: 683 48 79 145 196 264 343 23 Cuts at: 3753 4121 4162 4529 4914 4931 6383 6469 6481 Size: 368 41 367 385 17 1452 86 12 Cuts at: 6481 6827 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 683 385 368 367 346 343 264 196 145 86 79 48 41 23 17 12 BstZ17I GTA'TAC Cuts at: 3207 3207 Size: 6832 BtgI C'CryG_G Cuts at: 513 1065 2004 2182 2586 513 Size: 552 939 178 404 4759 Fragments arranged by size: 4759 939 552 404 178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3432 487 Size: 683 56 2206 3887 Fragments arranged by size: 3887 2206 683 56 BtsI GCAGTG_nn' Cuts at: 2627 4348 5142 5162 2627 Size: 1721 794 20 4297 Fragments arranged by size: 4297 1721 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6832 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6797 DraI TTT'AAA Cuts at: 4136 4136 Size: 6832 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2384 5839 809 Size: 507 1068 3455 1802 Fragments arranged by size: 3455 1802 1068 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 3579 4279 Size: 54 1425 50 275 18 379 1370 700 Cuts at: 4279 5168 6797 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1370 889 700 379 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4279 1537 Size: 275 2467 4090 Fragments arranged by size: 4090 2467 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 3456 4024 4040 4762 1353 Size: 1484 619 568 16 722 3423 Fragments arranged by size: 3423 1484 722 619 568 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2585 5419 5793 5939 387 Size: 1064 138 996 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 996 374 146 138 EcoICRI GAG'CTC Cuts at: 4017 4017 Size: 6832 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 3089 3196 3499 4089 4895 5492 Size: 393 407 1050 107 303 590 806 597 Cuts at: 5492 6279 6547 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1050 806 787 597 590 407 393 303 268 107 EcoO109I rG'GnC_Cy Cuts at: 2305 3015 2305 Size: 710 6122 EcoRI G'AATT_C Cuts at: 2450 4141 4519 2450 Size: 1691 378 4763 Fragments arranged by size: 4763 1691 378 FspI TGC'GCA Cuts at: 5338 5338 Size: 6832 HaeII r_GCGC'y Cuts at: 1678 2167 2758 2896 5979 1678 Size: 489 591 138 3083 2531 Fragments arranged by size: 3083 2531 591 489 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2583 2615 3110 3142 3681 Size: 32 135 32 1011 32 495 32 539 Cuts at: 3681 3713 3986 4001 4018 4033 4100 4132 5473 Size: 32 273 15 17 15 67 32 1341 Cuts at: 5473 5505 5547 5579 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1341 1011 539 495 273 135 67 42 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2069 2148 2859 3136 3259 4176 Size: 988 281 131 79 711 277 123 917 Cuts at: 4176 4422 669 Size: 246 3079 Fragments arranged by size: 3079 988 917 711 281 277 246 131 123 79 HindIII A'AGCT_T Cuts at: 748 748 Size: 6832 HpaI GTT'AAC Cuts at: 2148 4422 2148 Size: 2274 4558 KasI G'GCGC_C Cuts at: 2163 2163 Size: 6832 KpnI G_GTAC'C Cuts at: 3153 4157 3153 Size: 1004 5828 MfeI C'AATT_G Cuts at: 4431 4431 Size: 6832 MluI A'CGCG_T Cuts at: 6708 6708 Size: 6832 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5946 6130 1305 Size: 4641 184 2007 Fragments arranged by size: 4641 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2211 3581 10 Size: 54 1425 343 379 1370 3261 Fragments arranged by size: 3261 1425 1370 379 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 2679 4790 5149 5308 518 Size: 648 1323 190 2111 359 159 2042 Fragments arranged by size: 2111 2042 1323 648 359 190 159 MspA1I CmG'CkG Cuts at: 1606 2356 3067 4248 4925 6073 6318 1606 Size: 750 711 1181 677 1148 245 2120 Fragments arranged by size: 2120 1181 1148 750 711 677 245 NarI GG'CG_CC Cuts at: 2164 2164 Size: 6832 NcoI C'CATG_G Cuts at: 513 1065 2004 2182 513 Size: 552 939 178 5163 Fragments arranged by size: 5163 939 552 178 NdeI CA'TA_TG Cuts at: 387 387 Size: 6832 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6832 NotI GC'GGCC_GC Cuts at: 4279 4279 Size: 6832 NspI r_CATG'y Cuts at: 1132 1229 2923 3670 4190 5735 1132 Size: 97 1694 747 520 1545 2229 Fragments arranged by size: 2229 1694 1545 747 520 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6832 PciI A'CATG_T Cuts at: 2919 5731 2919 Size: 2812 4020 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6832 PfoI T'CCnGG_A Cuts at: 2610 3390 2610 Size: 780 6052 PmeI GTTT'AAAC Cuts at: 4136 4136 Size: 6832 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4901 4933 6414 6446 4901 Size: 32 1481 32 5287 Fragments arranged by size: 5287 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2305 Size: 6832 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6832 PsiI TTA'TAA Cuts at: 4402 4402 Size: 6832 PspOMI G'GGCC_C Cuts at: 3015 3015 Size: 6832 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3299 3331 3299 Size: 32 6800 PstI C_TGCA'G Cuts at: 830 2622 4184 830 Size: 1792 1562 3478 Fragments arranged by size: 3478 1792 1562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6832 PvuII CAG'CTG Cuts at: 2356 2356 Size: 6832 SacI G_AGCT'C Cuts at: 4019 4019 Size: 6832 SalI G'TCGA_C Cuts at: 1936 2067 4174 1936 Size: 131 2107 4594 Fragments arranged by size: 4594 2107 131 SapI GCTCTTCn'nnn_ Cuts at: 4024 4024 Size: 6832 SbfI CC_TGCA'GG Cuts at: 4184 4184 Size: 6832 ScaI AGT'ACT Cuts at: 2721 4090 5080 6792 2721 Size: 1369 990 1712 2761 Fragments arranged by size: 2761 1712 1369 990 SfcI C'TryA_G Cuts at: 826 1045 2618 4180 5315 5996 6187 826 Size: 219 1573 1562 1135 681 191 1471 Fragments arranged by size: 1573 1562 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3578 3578 Size: 6832 SfoI GGC'GCC Cuts at: 2165 2165 Size: 6832 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6832 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6832 SmaI CCC'GGG Cuts at: 1872 4159 1872 Size: 2287 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 3961 4936 5837 6099 820 Size: 197 926 345 1673 975 901 262 1553 Fragments arranged by size: 1673 1553 975 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 4623 493 Size: 4130 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6832 SphI G_CATG'C Cuts at: 1132 4190 1132 Size: 3058 3774 SspI AAT'ATT Cuts at: 5 52 4756 5 Size: 47 4704 2081 Fragments arranged by size: 4704 2081 47 StuI AGG'CCT Cuts at: 1517 2543 1517 Size: 1026 5806 StyI C'CwwG_G Cuts at: 513 1065 2004 2182 2838 3001 3582 4271 513 Size: 552 939 178 656 163 581 689 3074 Fragments arranged by size: 3074 939 689 656 581 552 178 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2524 2528 4859 4876 5029 5214 5659 Size: 1255 944 4 2331 17 153 185 445 Cuts at: 5659 325 Size: 1498 Fragments arranged by size: 2331 1498 1255 944 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2719 4088 5078 Size: 274 80 33 51 1443 742 1369 990 Cuts at: 5078 6790 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1369 990 742 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2436 2468 2606 2638 6792 6824 2436 Size: 32 138 32 4154 32 2444 Fragments arranged by size: 4154 2444 138 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6832 XbaI T'CTAG_A Cuts at: 4168 4168 Size: 6832 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6832 XmaI C'CCGG_G Cuts at: 1870 4157 1870 Size: 2287 4545 XmnI GAAnn'nnTTC Cuts at: 4961 4961 Size: 6832 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2032 4640 276 Size: 53 83 186 936 498 2608 2468 Fragments arranged by size: 2608 2468 936 498 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SexAI SrfI SwaI XcmI