(Linear) (Six-base) MAPSORT of: KIBB9982.seq Check: 3153 from: 1 to: 2127 KIBB9982.orf With 182 enzymes: SgfI * December 27, 2011 16:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 680 2127 Size: 680 1447 AatII G_ACGT'C Cuts at: 0 29 2127 Size: 29 2098 AccI GT'mk_AC Cuts at: 0 63 212 1201 2127 Size: 63 149 989 926 Fragments arranged by size: 989 926 149 63 Acc65I G'GTAC_C Cuts at: 0 1144 2127 Size: 1144 983 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1191 2084 2127 Size: 1191 893 43 Fragments arranged by size: 1191 893 43 AfeI AGC'GCT Cuts at: 0 889 2127 Size: 889 1238 AflIII A'CryG_T Cuts at: 0 914 2127 Size: 914 1213 AhdI GACnn_n'nnGTC Cuts at: 0 2109 2127 Size: 2109 18 AlwNI CAG_nnn'CTG Cuts at: 0 621 2127 Size: 621 1506 ApaI G_GGCC'C Cuts at: 0 1014 2127 Size: 1014 1113 ApaLI G'TGCA_C Cuts at: 0 414 1099 2127 Size: 414 685 1028 Fragments arranged by size: 1028 685 414 ApoI r'AATT_y Cuts at: 0 337 445 2127 Size: 337 108 1682 Fragments arranged by size: 1682 337 108 BamHI G'GATC_C Cuts at: 0 777 922 2116 2127 Size: 777 145 1194 11 Fragments arranged by size: 1194 777 145 11 BanI G'GyrC_C Cuts at: 0 158 1144 2127 Size: 158 986 983 Fragments arranged by size: 986 983 158 BanII G_rGCy'C Cuts at: 0 1014 1939 2014 2127 Size: 1014 925 75 113 Fragments arranged by size: 1014 925 113 75 BbeI G_GCGC'C Cuts at: 0 162 2127 Size: 162 1965 BbsI GAAGACnn'nnnn_ Cuts at: 0 1853 2127 Size: 1853 274 BbvCI CC'TCA_GC Cuts at: 0 397 1799 2127 Size: 397 1402 328 Fragments arranged by size: 1402 397 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1303 1337 2127 Size: 1303 34 790 Fragments arranged by size: 1303 790 34 BclI T'GATC_A Cuts at: 0 767 2127 Size: 767 1360 BglI GCCn_nnn'nGGC Cuts at: 0 83 1573 2127 Size: 83 1490 554 Fragments arranged by size: 1490 554 83 BglII A'GATC_T Cuts at: 0 1118 1748 2127 Size: 1118 630 379 Fragments arranged by size: 1118 630 379 BlpI GC'TnA_GC Cuts at: 0 1966 2127 Size: 1966 161 Bme1580I G_kGCm'C Cuts at: 0 418 1004 1014 1103 1195 2127 Size: 418 586 10 89 92 932 Fragments arranged by size: 932 586 418 92 89 10 BmrI ACTGGGnnnn_n' Cuts at: 0 333 405 1008 2127 Size: 333 72 603 1119 Fragments arranged by size: 1119 603 333 72 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 270 2127 Size: 270 1857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1084 1494 2127 Size: 34 1050 410 633 Fragments arranged by size: 1050 633 410 34 Bpu10I CC'TnA_GC Cuts at: 0 397 1044 1799 2127 Size: 397 647 755 328 Fragments arranged by size: 755 647 397 328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 1941 2127 Size: 304 1637 186 Fragments arranged by size: 1637 304 186 BsaI GGTCTCn'nnnn_ Cuts at: 0 1174 2127 Size: 1174 953 BsaHI Gr'CG_yC Cuts at: 0 26 159 2127 Size: 26 133 1968 Fragments arranged by size: 1968 133 26 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 601 631 2127 Size: 601 30 1496 Fragments arranged by size: 1496 601 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 617 814 1240 1405 1474 1492 1497 1795 Size: 617 197 426 165 69 18 5 298 Cuts at: 1795 2058 2061 2064 2127 Size: 263 3 3 63 Fragments arranged by size: 617 426 298 263 197 165 69 63 18 5 3 3 BseYI C'CCAG_C Cuts at: 0 133 2092 2127 Size: 133 1959 35 Fragments arranged by size: 1959 133 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 652 2127 Size: 652 1475 BsiHKAI G_wGCw'C Cuts at: 0 418 1103 2014 2127 Size: 418 685 911 113 Fragments arranged by size: 911 685 418 113 BsmI GAATG_Cn' Cuts at: 0 1401 2127 Size: 1401 726 Bsp1286I G_dGCh'C Cuts at: 0 418 1004 1014 1103 1195 1939 2014 2127 Size: 418 586 10 89 92 744 75 113 Fragments arranged by size: 744 586 418 113 92 89 75 10 BspHI T'CATG_A Cuts at: 0 950 2127 Size: 950 1177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 680 2127 Size: 680 1447 BsrBI CCG'CTC Cuts at: 0 1068 2127 Size: 1068 1059 BsrDI GCAATG_nn' Cuts at: 0 408 2127 Size: 408 1719 BssSI C'ACGA_G Cuts at: 0 629 1139 2127 Size: 629 510 988 Fragments arranged by size: 988 629 510 BstEII G'GTnAC_C Cuts at: 0 370 2127 Size: 370 1757 BstXI CCAn_nnnn'nTGG Cuts at: 0 517 2127 Size: 517 1610 BstYI r'GATC_y Cuts at: 0 650 698 777 922 1118 1382 1725 1748 Size: 650 48 79 145 196 264 343 23 Cuts at: 1748 2116 2127 Size: 368 11 Fragments arranged by size: 650 368 343 264 196 145 79 48 23 11 BstZ17I GTA'TAC Cuts at: 0 1202 2127 Size: 1202 925 BtgI C'CryG_G Cuts at: 0 177 581 2127 Size: 177 404 1546 Fragments arranged by size: 1546 404 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1427 2127 Size: 1427 700 BtsI GCAGTG_nn' Cuts at: 0 622 2127 Size: 622 1505 DrdI GACnn_nn'nnGTC Cuts at: 0 379 2127 Size: 379 1748 EaeI y'GGCC_r Cuts at: 0 204 1574 2127 Size: 204 1370 553 Fragments arranged by size: 1370 553 204 EarI CTCTTCn'nnn_ Cuts at: 0 832 1451 2019 2035 2127 Size: 832 619 568 16 92 Fragments arranged by size: 832 619 568 92 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 580 2127 Size: 580 1547 EcoICRI GAG'CTC Cuts at: 0 2012 2127 Size: 2012 115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1084 1191 1494 2084 2127 Size: 34 1050 107 303 590 43 Fragments arranged by size: 1050 590 303 107 43 34 EcoO109I rG'GnC_Cy Cuts at: 0 300 1010 2127 Size: 300 710 1117 Fragments arranged by size: 1117 710 300 EcoRI G'AATT_C Cuts at: 0 445 2127 Size: 445 1682 HaeII r_GCGC'y Cuts at: 0 162 753 891 2127 Size: 162 591 138 1236 Fragments arranged by size: 1236 591 162 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 578 610 1105 1137 1676 1708 1981 1996 Size: 578 32 495 32 539 32 273 15 Cuts at: 1996 2013 2028 2095 2127 Size: 17 15 67 32 Fragments arranged by size: 578 539 495 273 67 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 0 64 143 854 1131 1254 2127 Size: 64 79 711 277 123 873 Fragments arranged by size: 873 711 277 123 79 64 HpaI GTT'AAC Cuts at: 0 143 2127 Size: 143 1984 KasI G'GCGC_C Cuts at: 0 158 2127 Size: 158 1969 KpnI G_GTAC'C Cuts at: 0 1148 2127 Size: 1148 979 MscI TGG'CCA Cuts at: 0 206 1576 2127 Size: 206 1370 551 Fragments arranged by size: 1370 551 206 MslI CAynn'nnrTG Cuts at: 0 484 674 2127 Size: 484 190 1453 Fragments arranged by size: 1453 484 190 MspA1I CmG'CkG Cuts at: 0 351 1062 2127 Size: 351 711 1065 Fragments arranged by size: 1065 711 351 NarI GG'CG_CC Cuts at: 0 159 2127 Size: 159 1968 NcoI C'CATG_G Cuts at: 0 177 2127 Size: 177 1950 NspI r_CATG'y Cuts at: 0 918 1665 2127 Size: 918 747 462 Fragments arranged by size: 918 747 462 PciI A'CATG_T Cuts at: 0 914 2127 Size: 914 1213 PfoI T'CCnGG_A Cuts at: 0 605 1385 2127 Size: 605 780 742 Fragments arranged by size: 780 742 605 PpuMI rG'GwC_Cy Cuts at: 0 300 2127 Size: 300 1827 PspOMI G'GGCC_C Cuts at: 0 1010 2127 Size: 1010 1117 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1294 1326 2127 Size: 1294 32 801 Fragments arranged by size: 1294 801 32 PstI C_TGCA'G Cuts at: 0 617 2127 Size: 617 1510 PvuII CAG'CTG Cuts at: 0 351 2127 Size: 351 1776 SacI G_AGCT'C Cuts at: 0 2014 2127 Size: 2014 113 SalI G'TCGA_C Cuts at: 0 62 2127 Size: 62 2065 SapI GCTCTTCn'nnn_ Cuts at: 0 2019 2127 Size: 2019 108 ScaI AGT'ACT Cuts at: 0 716 2085 2127 Size: 716 1369 42 Fragments arranged by size: 1369 716 42 SfcI C'TryA_G Cuts at: 0 613 2127 Size: 613 1514 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1573 2127 Size: 1573 554 SfoI GGC'GCC Cuts at: 0 160 2127 Size: 160 1967 SmlI C'TyrA_G Cuts at: 0 283 1956 2127 Size: 283 1673 171 Fragments arranged by size: 1673 283 171 StuI AGG'CCT Cuts at: 0 538 2127 Size: 538 1589 StyI C'CwwG_G Cuts at: 0 177 833 996 1577 2127 Size: 177 656 163 581 550 Fragments arranged by size: 656 581 550 177 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 519 523 2127 Size: 519 4 1604 Fragments arranged by size: 1604 519 4 TatI w'GTAC_w Cuts at: 0 714 2083 2127 Size: 714 1369 44 Fragments arranged by size: 1369 714 44 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 431 463 601 633 2127 Size: 431 32 138 32 1494 Fragments arranged by size: 1494 431 138 32 32 ZraI GAC'GTC Cuts at: 0 27 2127 Size: 27 2100 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HpaI KasI KpnI MscI MslI MspA1I NarI NcoI NspI PciI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SalI SapI ScaI SfcI SfiI SfoI SmlI StuI StyI TaqII TatI TstI ZraI Enzymes that do not cut: AclI AflII AgeI AleI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BciVI BfrBI BmgBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstAPI BstBI Bsu36I ClaI CspCI DraI DraIII EagI EcoNI EcoRV FalI FseI FspI FspAI HindIII MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII SanDI SbfI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI