(Circular) (Six-base) MAPSORT of: pFN21AE1354.seq Check: 6912 from: 1 to: 7438 pFN21AE1354 7438 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2202 3960 2202 Size: 1758 5680 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4458 4781 1937 Size: 2521 323 4594 Fragments arranged by size: 4594 2521 323 Acc65I G'GTAC_C Cuts at: 4759 4759 Size: 7438 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3332 4094 5501 6885 7153 1632 Size: 1700 762 1407 1384 268 1917 Fragments arranged by size: 1917 1700 1407 1384 762 268 AfeI AGC'GCT Cuts at: 2349 2349 Size: 7438 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 3663 6337 7314 1203 Size: 2460 2674 977 1327 Fragments arranged by size: 2674 2460 1327 977 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 3534 4507 4699 6167 3534 Size: 973 192 1468 4805 Fragments arranged by size: 4805 1468 973 192 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7438 AlwNI CAG_nnn'CTG Cuts at: 2998 3889 5181 6753 2998 Size: 891 1292 1572 3683 Fragments arranged by size: 3683 1572 1292 891 ApaI G_GGCC'C Cuts at: 3993 4365 4670 3993 Size: 372 305 6761 Fragments arranged by size: 6761 372 305 ApaLI G'TGCA_C Cuts at: 2321 3026 5495 6651 2321 Size: 705 2469 1156 3108 Fragments arranged by size: 3108 2469 1156 705 ApoI r'AATT_y Cuts at: 1493 4747 4973 5125 7234 1493 Size: 3254 226 152 2109 1697 Fragments arranged by size: 3254 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 4364 4763 1870 Size: 73 2421 399 4545 Fragments arranged by size: 4545 2421 399 73 BamHI G'GATC_C Cuts at: 2928 3996 4768 5135 2928 Size: 1068 772 367 5231 Fragments arranged by size: 5231 1068 772 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2520 3486 3892 4654 4685 Size: 325 217 627 733 966 406 762 31 Cuts at: 4685 4759 6214 618 Size: 74 1455 1842 Fragments arranged by size: 1842 1455 966 762 733 627 406 325 217 74 31 BanII G_rGCy'C Cuts at: 1391 2250 2511 3444 3993 4365 4670 4757 1391 Size: 859 261 933 549 372 305 87 4072 Fragments arranged by size: 4072 933 859 549 372 305 261 87 BbeI G_GCGC'C Cuts at: 4658 4658 Size: 7438 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6467 BbvCI CC'TCA_GC Cuts at: 2648 3101 4596 2648 Size: 453 1495 5490 Fragments arranged by size: 5490 1495 453 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2197 2231 3377 3411 5629 5663 1903 Size: 34 260 34 1146 34 2218 34 3678 Fragments arranged by size: 3678 2218 1146 260 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5330 6540 5330 Size: 1210 6228 BclI T'GATC_A Cuts at: 1548 1548 Size: 7438 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3767 3974 4652 Size: 107 122 71 631 605 2095 207 678 Cuts at: 4652 6049 136 Size: 1397 1525 Fragments arranged by size: 2095 1525 1397 678 631 605 207 122 107 71 BglII A'GATC_T Cuts at: 3495 7433 3495 Size: 3938 3500 BlpI GC'TnA_GC Cuts at: 4064 4855 4064 Size: 791 6647 Bme1580I G_kGCm'C Cuts at: 1790 2325 2967 3030 3993 4365 4670 4683 4690 Size: 535 642 63 963 372 305 13 7 Cuts at: 4690 5499 6655 1790 Size: 809 1156 2573 Fragments arranged by size: 2573 1156 963 809 642 535 372 305 63 13 7 BmgBI CAC'GTC Cuts at: 1318 4405 1318 Size: 3087 4351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2125 5169 6127 Size: 416 53 83 399 24 708 3044 958 Cuts at: 6127 442 Size: 1753 Fragments arranged by size: 3044 1753 958 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2264 2264 Size: 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2942 2972 3020 3083 3248 3572 3593 Size: 1049 654 30 48 63 165 324 21 Cuts at: 3593 3641 3938 4073 4145 6098 1239 Size: 48 297 135 72 1953 2579 Fragments arranged by size: 2579 1953 1049 654 324 297 165 135 72 63 48 48 30 21 Bpu10I CC'TnA_GC Cuts at: 2295 2648 3101 4596 2295 Size: 353 453 1495 5137 Fragments arranged by size: 5137 1495 453 353 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 5563 6428 6726 2234 Size: 3329 865 298 2946 Fragments arranged by size: 3329 2946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2389 2483 3148 4511 6101 882 Size: 617 890 94 665 1363 1590 2219 Fragments arranged by size: 2219 1590 1363 890 665 617 94 BsaAI yAC'GTr Cuts at: 493 1204 5229 493 Size: 711 4025 2702 Fragments arranged by size: 4025 2702 711 BsaBI GATnn'nnATC Cuts at: 7432 7432 Size: 7438 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2373 3513 3741 4278 Size: 53 83 186 936 840 1140 228 537 Cuts at: 4278 4319 4655 4674 5245 5627 6262 275 Size: 41 336 19 571 382 635 1451 Fragments arranged by size: 1451 1140 936 840 635 571 537 382 336 228 186 83 53 41 19 BsaWI w'CCGG_w Cuts at: 2841 5871 6269 6543 6690 7101 2841 Size: 3030 398 274 147 411 3178 Fragments arranged by size: 3178 3030 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3449 3479 1178 Size: 30 165 30 2046 30 5137 Fragments arranged by size: 5137 2046 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3212 3701 3935 4028 4382 4613 1186 Size: 2026 489 234 93 354 231 4011 Fragments arranged by size: 4011 2026 489 354 234 231 93 BseYI C'CCAG_C Cuts at: 2048 4209 4357 6641 7137 2048 Size: 2161 148 2284 496 2349 Fragments arranged by size: 2349 2284 2161 496 148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2579 3065 3575 3902 1163 Size: 573 843 486 510 327 4699 Fragments arranged by size: 4699 843 573 510 486 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4417 4888 5649 6677 1540 Size: 275 186 2416 471 761 1028 2301 Fragments arranged by size: 2416 2301 1028 761 471 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2883 3030 3444 3951 4197 5499 5584 6655 Size: 558 147 414 507 246 1302 85 1071 Cuts at: 6655 2325 Size: 3108 Fragments arranged by size: 3108 1302 1071 558 507 414 246 147 85 BsmI GAATG_Cn' Cuts at: 4949 5042 4949 Size: 93 7345 BsmBI CGTCTCn'nnnn_ Cuts at: 2235 3323 2235 Size: 1088 6350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2325 2511 2883 2967 3030 3444 Size: 399 460 75 186 372 84 63 414 Cuts at: 3444 3951 3993 4197 4365 4670 4683 4690 4757 Size: 507 42 204 168 305 13 7 67 Cuts at: 4757 5499 5584 6655 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 507 460 414 399 372 305 204 186 168 85 84 75 67 63 42 13 7 BspEI T'CCGG_A Cuts at: 7101 7101 Size: 7438 BspHI T'CATG_A Cuts at: 4446 5327 7057 4446 Size: 881 1730 4827 Fragments arranged by size: 4827 1730 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2202 3960 4128 4793 844 Size: 1292 66 1758 168 665 3489 Fragments arranged by size: 3489 1758 1292 665 168 66 BsrBI CCG'CTC Cuts at: 1394 1823 2287 2305 3232 5325 1394 Size: 429 464 18 927 2093 3507 Fragments arranged by size: 3507 2093 927 464 429 18 BsrDI GCAATG_nn' Cuts at: 65 1248 2068 3429 4230 5933 6107 65 Size: 1183 820 1361 801 1703 174 1396 Fragments arranged by size: 1703 1396 1361 1183 820 801 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2613 3018 3108 4416 6082 6269 1700 Size: 110 803 405 90 1308 1666 187 2869 Fragments arranged by size: 2869 1666 1308 803 405 187 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7438 BssHII G'CGCG_C Cuts at: 1925 3610 1925 Size: 1685 5753 BssSI C'ACGA_G Cuts at: 3278 3371 5498 6510 3278 Size: 93 2127 1012 4206 Fragments arranged by size: 4206 2127 1012 93 BstAPI GCAn_nnn'nTGC Cuts at: 794 3892 794 Size: 3098 4340 BstBI TT'CG_AA Cuts at: 2688 6242 2688 Size: 3554 3884 BstEII G'GTnAC_C Cuts at: 1184 2289 1184 Size: 1105 6333 BstXI CCAn_nnnn'nTGG Cuts at: 2725 3985 2725 Size: 1260 6178 BstYI r'GATC_y Cuts at: 1972 2844 2928 3216 3495 3996 4263 4768 5135 Size: 872 84 288 279 501 267 505 367 Cuts at: 5135 5520 5537 6989 7075 7087 7433 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 872 505 501 385 367 346 288 279 267 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2456 2456 Size: 7438 BtgI C'CryG_G Cuts at: 513 1065 2004 2481 3645 4188 4710 513 Size: 552 939 477 1164 543 522 3241 Fragments arranged by size: 3241 1164 939 552 543 522 477 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4534 4627 487 Size: 683 56 3308 93 3298 Fragments arranged by size: 3308 3298 683 93 56 BtsI GCAGTG_nn' Cuts at: 4954 5748 5768 4954 Size: 794 20 6624 Fragments arranged by size: 6624 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7403 DraI TTT'AAA Cuts at: 4742 4742 Size: 7438 DraIII CAC_nnn'GTG Cuts at: 3486 3486 Size: 7438 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2663 2785 6445 809 Size: 507 1347 122 3660 1802 Fragments arranged by size: 3660 1802 1347 507 122 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2496 2907 3333 Size: 54 1425 50 275 18 666 411 426 Cuts at: 3333 3768 4885 5774 7403 8 Size: 435 1117 889 1629 43 Fragments arranged by size: 1629 1425 1117 889 666 435 426 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2481 5368 1353 Size: 1041 87 2887 3423 Fragments arranged by size: 3423 2887 1041 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6025 6399 6545 387 Size: 1064 138 4436 374 146 1280 Fragments arranged by size: 4436 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3442 3442 Size: 7438 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2942 2972 3020 3083 3248 3332 Size: 393 656 654 30 48 63 165 84 Cuts at: 3332 3572 3593 3641 3938 4073 4094 4145 5501 Size: 240 21 48 297 135 21 51 1356 Cuts at: 5501 6098 6885 7153 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1356 787 656 654 597 393 297 268 240 165 135 84 63 51 48 48 30 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 2198 2888 3917 2198 Size: 690 1029 5719 Fragments arranged by size: 5719 1029 690 EcoO109I rG'GnC_Cy Cuts at: 2125 3652 3697 3989 3990 4047 4153 4361 4666 Size: 1527 45 292 1 57 106 208 305 Cuts at: 4666 4667 2125 Size: 1 4896 Fragments arranged by size: 4896 1527 305 292 208 106 57 45 1 1 EcoRI G'AATT_C Cuts at: 4747 5125 4747 Size: 378 7060 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2528 2528 Size: 7438 FspI TGC'GCA Cuts at: 3399 5944 3399 Size: 2545 4893 HaeII r_GCGC'y Cuts at: 1678 2070 2351 3645 4358 4658 6585 1678 Size: 392 281 1294 713 300 1927 2531 Fragments arranged by size: 2531 1927 1294 713 392 300 281 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 3520 3552 4493 Size: 32 135 32 689 32 1227 32 941 Cuts at: 4493 4525 4685 4717 6079 6111 6153 6185 1373 Size: 32 160 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1362 1227 941 689 160 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2866 3478 4459 4782 5028 669 Size: 988 281 928 612 981 323 246 3079 Fragments arranged by size: 3079 988 981 928 612 323 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7438 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KasI G'GCGC_C Cuts at: 4654 4654 Size: 7438 KpnI G_GTAC'C Cuts at: 4763 4763 Size: 7438 MfeI C'AATT_G Cuts at: 5037 5037 Size: 7438 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3966 6552 6736 1305 Size: 2661 2586 184 2007 Fragments arranged by size: 2661 2586 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2909 3770 10 Size: 54 1425 343 1077 861 3678 Fragments arranged by size: 3678 1425 1077 861 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 4037 4202 5396 5755 5914 518 Size: 648 1314 1557 165 1194 359 159 2042 Fragments arranged by size: 2042 1557 1314 1194 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2577 3061 3571 3589 3889 4063 4219 4595 Size: 971 484 510 18 300 174 156 376 Cuts at: 4595 4854 5531 6679 6924 1606 Size: 259 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 677 510 484 376 300 259 245 174 156 18 NarI GG'CG_CC Cuts at: 4655 4655 Size: 7438 NcoI C'CATG_G Cuts at: 513 1065 2004 3645 4188 513 Size: 552 939 1641 543 3763 Fragments arranged by size: 3763 1641 939 552 543 NdeI CA'TA_TG Cuts at: 387 387 Size: 7438 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 3667 4796 6341 1132 Size: 97 2438 1129 1545 2229 Fragments arranged by size: 2438 2229 1545 1129 97 PasI CC'CwG_GG Cuts at: 1348 2431 1348 Size: 1083 6355 PciI A'CATG_T Cuts at: 3663 6337 3663 Size: 2674 4764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2946 1108 Size: 1838 5600 PfoI T'CCnGG_A Cuts at: 2265 2469 2919 2979 3657 3915 4146 2265 Size: 204 450 60 678 258 231 5557 Fragments arranged by size: 5557 678 450 258 231 204 60 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5507 5539 7020 7052 4418 Size: 32 1057 32 1481 32 4804 Fragments arranged by size: 4804 1481 1057 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 3652 4047 2125 Size: 1527 395 5516 Fragments arranged by size: 5516 1527 395 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 5008 5008 Size: 7438 PspOMI G'GGCC_C Cuts at: 3989 4361 4666 3989 Size: 372 305 6761 Fragments arranged by size: 6761 372 305 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2018 2050 2018 Size: 32 7406 PstI C_TGCA'G Cuts at: 830 3087 4790 830 Size: 2257 1703 3478 Fragments arranged by size: 3478 2257 1703 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 2577 3061 3571 3589 3889 4063 4219 4595 2577 Size: 484 510 18 300 174 156 376 5420 Fragments arranged by size: 5420 510 484 376 300 174 156 18 SacI G_AGCT'C Cuts at: 3444 3444 Size: 7438 SalI G'TCGA_C Cuts at: 1936 4457 4780 1936 Size: 2521 323 4594 Fragments arranged by size: 4594 2521 323 SanDI GG'GwC_CC Cuts at: 4047 4047 Size: 7438 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 4637 5686 7398 4637 Size: 1049 1712 4677 Fragments arranged by size: 4677 1712 1049 SexAI A'CCwGG_T Cuts at: 4542 4734 4542 Size: 192 7246 SfcI C'TryA_G Cuts at: 826 1045 3083 4786 5921 6602 6793 826 Size: 219 2038 1703 1135 681 191 1471 Fragments arranged by size: 2038 1703 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3767 3767 Size: 7438 SfoI GGC'GCC Cuts at: 4656 4656 Size: 7438 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 4765 1872 Size: 2893 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 5542 6443 6705 820 Size: 197 926 306 3293 901 262 1553 Fragments arranged by size: 3293 1553 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4796 1132 Size: 3664 3774 SspI AAT'ATT Cuts at: 5 52 5362 5 Size: 47 5310 2081 Fragments arranged by size: 5310 2081 47 StuI AGG'CCT Cuts at: 1517 3269 3284 3377 4379 1517 Size: 1752 15 93 1002 4576 Fragments arranged by size: 4576 1752 1002 93 15 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 3039 3645 4188 4281 4877 Size: 552 939 495 540 606 543 93 596 Cuts at: 4877 513 Size: 3074 Fragments arranged by size: 3074 939 606 596 552 543 540 495 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5465 5482 5635 5820 6265 325 Size: 1255 3885 17 153 185 445 1498 Fragments arranged by size: 3885 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4635 5684 7396 Size: 274 80 33 51 1443 2658 1049 1712 Cuts at: 7396 96 Size: 138 Fragments arranged by size: 2658 1712 1443 1049 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7398 7430 7398 Size: 32 7406 Tth111I GACn'n_nGTC Cuts at: 1316 2663 4544 1316 Size: 1347 1881 4210 Fragments arranged by size: 4210 1881 1347 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2917 2947 4186 4249 2917 Size: 30 1239 63 6106 Fragments arranged by size: 6106 1239 63 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7438 XmaI C'CCGG_G Cuts at: 1870 4763 1870 Size: 2893 4545 XmnI GAAnn'nnTTC Cuts at: 5567 5567 Size: 7438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SapI SrfI SwaI