Restriction map for
pFN21AE3582
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# NO CUT ENZYMES ( 111 enzymes) #
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AclI AcsI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI AspI AviII AvrII BbeI
BbsI BbvCI BcgI BciVI BclI
BfaI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HindIII HpaI KasI
KspI MaeI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 451 GACGTC GACGT^C
Acc65I 47 GGTACC G^GTACC
AccIII 407 TCCGGA T^CCGGA
AflII 511 CTTAAG C^TTAAG
AflIII 785 ACATGT A^C[GA][CT]GT
AgeI 314 ACCGGT A^CCGGT
AhdI 1066 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 387 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 47 GGTACC G^GTACC
AspEI 1066 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 702 GGATCC G^GATCC
BfrI 511 CTTAAG C^TTAAG
BglII 1490 AGATCT A^GATCT
BsaI 649 GAGACC GGTCTC(1/5)
BseAI 407 TCCGGA T^CCGGA
BseRI 1327 GAGGAG GAGGAG(10/8)
BsmBI 667 GAGACG CGTCTC(1/5)
BsmI 660 GAATGC GAATGC(1/-1)
BspEI 407 TCCGGA T^CCGGA
BspLU11I 785 ACATGT A^CATGT
BsrFI 314 ACCGGT [GA]^CCGG[CT]
Cfr10I 314 ACCGGT [GA]^CCGG[CT]
DsaI 141 CCATGG C^C[GA][CT]GG
Eam1105I 1066 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 364 GTCAAC GT[CT]^[GA]AC
HindII 364 GTCAAC GT[CT]^[GA]AC
KpnI 47 GGTACC GGTAC^C
MroI 407 TCCGGA T^CCGGA
MseI 512 TTAA T^TAA
MslI 1194 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 141 CCATGG C^CATGG
PflMI 1130 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 314 ACCGGT A^CCGGT
SacI 1342 GAGCTC GAGCT^C
SexAI 173 ACCTGGT A^CC[AT]GGT
SphI 1164 GCATGC GCATG^C
Tru9I 512 TTAA T^TAA
Tsp509I 771 AATT ^AATT
Van91I 1130 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 387 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 337 GTAGAC GT^[AC][GT]AC
738 GTCTAC
AcyI 451 GACGTC G[GA]^CG[CT]C
927 GACGCC
AlwNI 31 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1027 CAGTACCTG
BanI 47 GGTACC G^G[CT][GA]CC
1192 GGCACC
BbrPI 298 CACGTG CAC^GTG
859 CACGTG
BsaAI 298 CACGTG [CT]AC^GT[GA]
859 CACGTG
BsaHI 451 GACGTC G[GA]^CG[CT]C
927 GACGCC
BsaWI 314 ACCGGT [AT]^CCGG[AT]
407 TCCGGA
BspMI 74 ACCTGC ACCTGC(4/8)
1031 ACCTGC
CfoI 78 GCGC GCG^C
1656 GCGC
DdeI 1565 CTAAG C^T[ACGT]AG
1580 CTGAG
EarI 85 CTCTTC CTCTTC(1/4)
522 CTCTTC
FauI 536 CCCGC CCCGC(4/6)
1354 GCGGG
HhaI 78 GCGC GCG^C
1656 GCGC
Hin1I 451 GACGTC G[GA]^CG[CT]C
927 GACGCC
HinP1I 78 GCGC G^CGC
1656 GCGC
Ksp632I 85 CTCTTC CTCTTC(1/4)
522 CTCTTC
NspI 785 ACATGT [GA]CATG^[CT]
1164 GCATGC
PmaCI 298 CACGTG CAC^GTG
859 CACGTG
PmlI 298 CACGTG CAC^GTG
859 CACGTG
PstI 37 CTGCAG CTGCA^G
710 CTGCAG
SfcI 37 CTGCAG C^T[GA][CT]AG
710 CTGCAG
SmaI 14 CCCGGG CCC^GGG
239 CCCGGG
XmaCI 14 CCCGGG C^CCGGG
239 CCCGGG
XmaI 14 CCCGGG C^CCGGG
239 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I Cfr13I DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII