(Circular) (Six-base) MAPSORT of: pFN21AE1362.seq Check: 1003 from: 1 to: 5641 pFN21AE1362 5641 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2605 2605 Size: 5641 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2510 2672 3704 5088 5356 1632 Size: 578 300 162 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1032 578 300 268 162 AfeI AGC'GCT Cuts at: 2405 2405 Size: 5641 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2188 2809 2857 3384 4956 2188 Size: 621 48 527 1572 2873 Fragments arranged by size: 2873 1572 621 527 48 ApaI G_GGCC'C Cuts at: 2509 2509 Size: 5641 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2500 2508 2870 2966 1870 Size: 73 557 8 362 96 4545 Fragments arranged by size: 4545 557 362 96 73 8 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2338 2366 2554 2962 4417 Size: 325 217 627 551 28 188 408 1455 Cuts at: 4417 618 Size: 1842 Fragments arranged by size: 1842 1455 627 551 408 325 217 188 28 BanII G_rGCy'C Cuts at: 1391 2137 2171 2509 2886 2960 1391 Size: 746 34 338 377 74 4072 Fragments arranged by size: 4072 746 377 338 74 34 BbeI G_GCGC'C Cuts at: 2370 2370 Size: 5641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2440 4252 136 Size: 107 122 71 631 605 768 1812 1525 Fragments arranged by size: 1812 1525 768 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2463 2645 3058 2463 Size: 182 413 5046 Fragments arranged by size: 5046 413 182 Bme1580I G_kGCm'C Cuts at: 1790 2028 2509 2559 3702 4858 1790 Size: 238 481 50 1143 1156 2573 Fragments arranged by size: 2573 1156 1143 481 238 50 BmgBI CAC'GTC Cuts at: 1318 2176 1318 Size: 858 4783 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 2489 4301 1239 Size: 1133 117 1812 2579 Fragments arranged by size: 2579 1812 1133 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2690 4304 882 Size: 617 1191 1614 2219 Fragments arranged by size: 2219 1614 1191 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3448 3830 4465 Size: 53 83 186 936 834 1081 382 635 Cuts at: 4465 275 Size: 1451 Fragments arranged by size: 1451 1081 936 834 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2320 2414 2888 1186 Size: 1134 94 474 3939 Fragments arranged by size: 3939 1134 474 94 BseYI C'CCAG_C Cuts at: 2012 2109 2328 2427 2908 4844 5340 2012 Size: 97 219 99 481 1936 496 2313 Fragments arranged by size: 2313 1936 496 481 219 99 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 2843 1163 Size: 573 507 600 3961 Fragments arranged by size: 3961 600 573 507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2319 2440 2642 3091 3852 4880 Size: 275 186 318 121 202 449 761 1028 Cuts at: 4880 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 449 318 275 202 186 121 BsiHKAI G_wGCw'C Cuts at: 2886 3702 3787 4858 2886 Size: 816 85 1071 3669 Fragments arranged by size: 3669 1071 816 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2137 2171 2509 2559 2886 2960 Size: 399 238 109 34 338 50 327 74 Cuts at: 2960 3702 3787 4858 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 338 327 238 109 85 74 50 34 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2605 2996 844 Size: 1761 391 3489 Fragments arranged by size: 3489 1761 391 BsrBI CCG'CTC Cuts at: 1394 1823 2160 2374 3528 1394 Size: 429 337 214 1154 3507 Fragments arranged by size: 3507 1154 429 337 214 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2363 2439 4285 4472 1700 Size: 110 553 76 1846 187 2869 Fragments arranged by size: 2869 1846 553 187 110 76 BsrGI T'GTAC_A Cuts at: 96 2127 96 Size: 2031 3610 BssHII G'CGCG_C Cuts at: 1925 2290 2359 2672 1925 Size: 365 69 313 4894 Fragments arranged by size: 4894 365 313 69 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 2338 794 Size: 1544 4097 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2193 2971 3338 3723 3740 5192 5278 5290 Size: 221 778 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 778 385 367 346 221 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4150 Fragments arranged by size: 4150 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2130 2711 3157 3951 3971 2130 Size: 581 446 794 20 3800 Fragments arranged by size: 3800 794 581 446 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2338 2338 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2081 2525 4648 809 Size: 507 765 444 2123 1802 Fragments arranged by size: 2123 1802 765 507 444 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2254 2316 2639 Size: 54 1425 50 275 18 424 62 323 Cuts at: 2639 2937 3088 3977 5606 8 Size: 298 151 889 1629 43 Fragments arranged by size: 1629 1425 889 424 323 298 275 151 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2316 2639 3088 1537 Size: 275 504 323 449 4090 Fragments arranged by size: 4090 504 449 323 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 3571 1353 Size: 672 1546 3423 Fragments arranged by size: 3423 1546 672 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2408 4228 4602 4748 387 Size: 1064 138 819 1820 374 146 1280 Fragments arranged by size: 1820 1280 1064 819 374 146 138 EcoICRI GAG'CTC Cuts at: 2884 2884 Size: 5641 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2372 2489 2510 2672 3704 4301 Size: 393 578 162 117 21 162 1032 597 Cuts at: 4301 5088 5356 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1032 787 597 578 393 268 162 162 117 21 EcoNI CCTnn'n_nnAGG Cuts at: 2861 2897 2861 Size: 36 5605 EcoO109I rG'GnC_Cy Cuts at: 2505 2740 2505 Size: 235 5406 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 2288 2370 2407 2710 2735 2750 2818 2857 Size: 610 82 37 303 25 15 68 39 Cuts at: 2857 4788 1678 Size: 1931 2531 Fragments arranged by size: 2531 1931 610 303 82 68 39 37 25 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KasI G'GCGC_C Cuts at: 2366 2366 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2070 2547 4755 4939 1305 Size: 765 477 2208 184 2007 Fragments arranged by size: 2208 2007 765 477 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3599 3958 4117 518 Size: 648 2433 359 159 2042 Fragments arranged by size: 2433 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2215 2227 2344 2644 2806 3057 3734 Size: 406 203 12 117 300 162 251 677 Cuts at: 3734 4882 5127 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 406 300 251 245 203 162 117 12 NaeI GCC'GGC Cuts at: 2365 2365 Size: 5641 NarI GG'CG_CC Cuts at: 2367 2367 Size: 5641 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4150 Fragments arranged by size: 4150 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NgoMIV G'CCGG_C Cuts at: 2363 2363 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 2316 3088 2316 Size: 772 4869 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PfoI T'CCnGG_A Cuts at: 2445 2445 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2740 2740 Size: 5641 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2505 2505 Size: 5641 PspXI vC'TCGA_Gb Cuts at: 2870 2870 Size: 5641 PstI C_TGCA'G Cuts at: 830 2142 2184 2304 2718 2853 2993 830 Size: 1312 42 120 414 135 140 3478 Fragments arranged by size: 3478 1312 414 140 135 120 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2012 2215 2227 2806 2012 Size: 203 12 579 4847 Fragments arranged by size: 4847 579 203 12 SacI G_AGCT'C Cuts at: 2886 2886 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SanDI GG'GwC_CC Cuts at: 2740 2740 Size: 5641 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2138 2180 2300 2628 2714 2849 2989 Size: 219 1093 42 120 328 86 135 140 Cuts at: 2989 4124 4805 4996 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1093 681 328 219 191 140 135 120 86 42 SfoI GGC'GCC Cuts at: 2368 2368 Size: 5641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 2363 1700 Size: 663 4978 SmaI CCC'GGG Cuts at: 1872 2510 2968 1872 Size: 638 458 4545 Fragments arranged by size: 4545 638 458 SmlI C'TyrA_G Cuts at: 820 1017 1943 2870 3745 4646 4908 820 Size: 197 926 927 875 901 262 1553 Fragments arranged by size: 1553 927 926 901 875 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 2798 1517 Size: 1281 4360 StyI C'CwwG_G Cuts at: 513 1065 2004 2148 3080 513 Size: 552 939 144 932 3074 Fragments arranged by size: 3074 939 932 552 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2933 3668 3685 3838 4023 4468 Size: 1255 446 907 735 17 153 185 445 Cuts at: 4468 325 Size: 1498 Fragments arranged by size: 1498 1255 907 735 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2127 3887 5599 Size: 274 80 33 51 1443 150 1760 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1760 1712 1443 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2932 2932 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 2870 1943 Size: 927 4714 XmaI C'CCGG_G Cuts at: 1870 2508 2966 1870 Size: 638 458 4545 Fragments arranged by size: 4545 638 458 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI