################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI DraI DraIII DrdI DsaI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 291 CGCG CG^CG AccIII 483 TCCGGA T^CCGGA AlwI 473 GGATC GGATC(4/5) ApaI 817 GGGCCC GGGCC^C BanI 546 GGTGCC G^G[CT][GA]CC BsaWI 483 TCCGGA [AT]^CCGG[AT] BseAI 483 TCCGGA T^CCGGA BsiEI 336 CGGCCG CG[GA][CT]^CG BsiWI 461 CGTACG C^GTACG BsmFI 825 GGGAC GGGAC(10/14) BsmI 163 GAATGC GAATGC(1/-1) BspEI 483 TCCGGA T^CCGGA BspHI 137 TCATGA T^CATGA BspMI 503 ACCTGC ACCTGC(4/8) BsrBI 36 GAGCGG CCGCTC(-3/-3) BsrDI 534 CATTGC GCAATG(2/0) BsrFI 488 ACCGGC [GA]^CCGG[CT] BstUI 291 CGCG CG^CG Cfr10I 488 ACCGGC [GA]^CCGG[CT] CpoI 485 CGGACCG CG^G[AT]CCG EagI 336 CGGCCG C^GGCCG EarI 397 CTCTTC CTCTTC(1/4) EclXI 336 CGGCCG C^GGCCG Eco52I 336 CGGCCG C^GGCCG Ksp632I 397 CTCTTC CTCTTC(1/4) MaeII 441 ACGT A^CGT MroI 483 TCCGGA T^CCGGA MseI 635 TTAA T^TAA MslI 187 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 291 CGCG CG^CG PpuMI 125 AGGACCC [GA]G^G[AT]CC[CT] PvuII 94 CAGCTG CAG^CTG RcaI 137 TCATGA T^CATGA RsrII 485 CGGACCG CG^G[AT]CCG SfiI 267 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 771 AGGCCT AGG^CCT Tru9I 635 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AcyI 919 GGCGTC G[GA]^CG[CT]C 940 GGCGTC BbvCI 1267 CCTCAGC CCTCAGC(-5/-2) 1332 GCTGAGG BcgI 528 CGAGACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 554 GCAGAGAGATCG BsaHI 919 GGCGTC G[GA]^CG[CT]C 940 GGCGTC BstAPI 167 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 498 GCAGTACCTGC EcoT14I 260 CCAAGG C^C[AT][AT]GG 269 CCAAGG HaeII 83 AGCGCC [GA]GCGC^[CT] 491 GGCGCT HgaI 920 GCGTC GACGC(5/10) 941 GCGTC Hin1I 919 GGCGTC G[GA]^CG[CT]C 940 GGCGTC StyI 260 CCAAGG C^C[AT][AT]GG 269 CCAAGG TspRI 592 CACTG CA[GC]TG[ACGT][ACGT]^ 875 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AfaI AluI Alw44I AlwNI AocI ApaLI AspHI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI TaqI TseI Tsp45I TthHB8I Van91I XmaCI XmaI