(Circular) (Six-base) MAPSORT of: pFN21AE1519.seq Check: 6691 from: 1 to: 6097 pFN21AE1519 6097 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 3396 4224 4597 1563 Size: 1833 828 373 3063 Fragments arranged by size: 3063 1833 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4160 5544 5812 1632 Size: 2528 1384 268 1917 Fragments arranged by size: 2528 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2910 1184 Size: 1726 4371 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2570 2602 2570 Size: 32 6065 AlwNI CAG_nnn'CTG Cuts at: 2509 2810 2921 2942 2963 3005 3068 3840 5412 Size: 301 111 21 21 42 63 772 1572 Cuts at: 5412 2509 Size: 3194 Fragments arranged by size: 3194 1572 772 301 111 63 42 21 21 ApaI G_GGCC'C Cuts at: 2826 2826 Size: 6097 ApaLI G'TGCA_C Cuts at: 3140 3182 3308 4154 5310 3140 Size: 42 126 846 1156 3927 Fragments arranged by size: 3927 1156 846 126 42 ApoI r'AATT_y Cuts at: 1493 3406 3632 3784 5893 1493 Size: 1913 226 152 2109 1697 Fragments arranged by size: 2109 1913 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2347 2827 3026 3133 3217 3239 Size: 73 81 323 480 199 107 84 22 Cuts at: 3239 3422 1870 Size: 183 4545 Fragments arranged by size: 4545 480 323 199 183 107 84 81 73 22 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2551 3418 4873 618 Size: 325 217 627 764 867 1455 1842 Fragments arranged by size: 1842 1455 867 764 627 325 217 BanII G_rGCy'C Cuts at: 1391 2162 2529 2826 3416 1391 Size: 771 367 297 590 4072 Fragments arranged by size: 4072 771 590 367 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5126 BbvCI CC'TCA_GC Cuts at: 3273 3338 3273 Size: 65 6032 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2522 2548 2556 2582 4288 4322 1903 Size: 34 585 26 8 26 1706 34 3678 Fragments arranged by size: 3678 1706 585 34 34 26 26 8 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2028 2270 2279 Size: 107 122 71 631 605 356 242 9 Cuts at: 2279 2349 2734 2821 4708 136 Size: 70 385 87 1887 1525 Fragments arranged by size: 1887 1525 631 605 385 356 242 122 107 87 71 70 9 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 2826 3144 3186 3312 4158 5314 1790 Size: 1036 318 42 126 846 1156 2573 Fragments arranged by size: 2573 1156 1036 846 318 126 42 BmgBI CAC'GTC Cuts at: 1318 2447 1318 Size: 1129 4968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2873 3828 4786 Size: 416 53 83 399 24 1456 955 958 Cuts at: 4786 442 Size: 1753 Fragments arranged by size: 1753 1456 958 955 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2612 2841 3003 3027 4757 1239 Size: 890 483 229 162 24 1730 2579 Fragments arranged by size: 2579 1730 890 483 229 162 24 Bpu10I CC'TnA_GC Cuts at: 3273 3338 3273 Size: 65 6032 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2105 2528 2603 4760 882 Size: 617 606 423 75 2157 2219 Fragments arranged by size: 2219 2157 617 606 423 75 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2925 2946 3904 4286 Size: 53 83 186 936 1392 21 958 382 Cuts at: 4286 4921 275 Size: 635 1451 Fragments arranged by size: 1451 1392 958 936 635 382 186 83 53 21 BsaWI w'CCGG_w Cuts at: 2488 4530 4928 5202 5349 5760 2488 Size: 2042 398 274 147 411 2825 Fragments arranged by size: 2825 2042 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2570 2600 2655 Size: 30 165 30 741 30 396 30 55 Cuts at: 2655 2685 1178 Size: 30 4590 Fragments arranged by size: 4590 741 396 165 55 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2050 2390 1186 Size: 861 3 340 4893 Fragments arranged by size: 4893 861 340 3 BseYI C'CCAG_C Cuts at: 2289 2666 2838 2914 2935 2956 2998 3145 5300 Size: 377 172 76 21 21 42 147 2155 Cuts at: 5300 5796 2289 Size: 496 2590 Fragments arranged by size: 2590 2155 496 377 172 147 76 42 21 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2344 3547 4308 5336 1540 Size: 275 186 343 1203 761 1028 2301 Fragments arranged by size: 2301 1203 1028 761 343 275 186 BsiHKAI G_wGCw'C Cuts at: 3144 3186 3312 4158 4243 5314 3144 Size: 42 126 846 85 1071 3927 Fragments arranged by size: 3927 1071 846 126 85 42 BsiWI C'GTAC_G Cuts at: 2466 2466 Size: 6097 BsmI GAATG_Cn' Cuts at: 2174 3608 3701 2174 Size: 1434 93 4570 Fragments arranged by size: 4570 1434 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2529 2826 3144 3186 3312 3416 Size: 399 372 367 297 318 42 126 104 Cuts at: 3416 4158 4243 5314 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 367 318 297 126 104 85 42 BspEI T'CCGG_A Cuts at: 2488 5760 2488 Size: 3272 2825 BspHI T'CATG_A Cuts at: 2142 3986 5716 2142 Size: 1844 1730 2523 Fragments arranged by size: 2523 1844 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 3452 844 Size: 1673 935 3489 Fragments arranged by size: 3489 1673 935 BsrBI CCG'CTC Cuts at: 1394 1823 2043 3984 1394 Size: 429 220 1941 3507 Fragments arranged by size: 3507 1941 429 220 BsrDI GCAATG_nn' Cuts at: 65 1248 2538 4592 4766 65 Size: 1183 1290 2054 174 1396 Fragments arranged by size: 2054 1396 1290 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2493 4741 4928 1700 Size: 110 683 2248 187 2869 Fragments arranged by size: 2869 2248 683 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 2178 2509 794 Size: 1384 331 4382 Fragments arranged by size: 4382 1384 331 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092 Size: 1455 367 385 17 1452 86 12 346 Cuts at: 6092 1972 Size: 1977 Fragments arranged by size: 1977 1455 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2818 3168 3210 2818 Size: 350 42 5705 Fragments arranged by size: 5705 350 42 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2003 487 Size: 683 56 777 4581 Fragments arranged by size: 4581 777 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2341 2517 2735 Size: 54 1425 50 275 18 511 176 218 Cuts at: 2735 3544 4433 6062 8 Size: 809 889 1629 43 Fragments arranged by size: 1629 1425 889 809 511 275 218 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2341 3544 1537 Size: 275 529 1203 4090 Fragments arranged by size: 4090 1203 529 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2408 4027 1353 Size: 1055 1619 3423 Fragments arranged by size: 3423 1619 1055 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2591 4684 5058 5204 387 Size: 1064 138 1002 2093 374 146 1280 Fragments arranged by size: 2093 1280 1064 1002 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2612 2841 3003 3027 4160 4757 Size: 393 497 483 229 162 24 1133 597 Cuts at: 4757 5544 5812 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1133 787 597 497 483 393 268 229 162 24 EcoO109I rG'GnC_Cy Cuts at: 2131 2281 2807 2822 2823 3130 3172 3214 3342 Size: 150 526 15 1 307 42 42 128 Cuts at: 3342 2131 Size: 4886 Fragments arranged by size: 4886 526 307 150 128 42 42 15 1 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2638 2638 Size: 6097 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2092 2500 5244 1678 Size: 414 408 2744 2531 Fragments arranged by size: 2744 2531 414 408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2502 2707 2739 3193 Size: 32 135 32 898 32 205 32 454 Cuts at: 3193 3225 4738 4770 4812 4844 1373 Size: 32 1513 32 42 32 2626 Fragments arranged by size: 2626 1513 898 454 205 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5211 5395 1305 Size: 3906 184 2007 Fragments arranged by size: 3906 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 4055 4414 4573 518 Size: 648 1030 1859 359 159 2042 Fragments arranged by size: 2042 1859 1030 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 2713 2743 3113 3513 4190 5338 5583 Size: 495 612 30 370 400 677 1148 245 Cuts at: 5583 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 612 495 400 370 245 30 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 3455 5000 1132 Size: 97 2226 1545 2229 Fragments arranged by size: 2229 2226 1545 97 PasI CC'CwG_GG Cuts at: 1348 2687 2963 3068 3089 3176 3197 3365 1348 Size: 1339 276 105 21 87 21 168 4080 Fragments arranged by size: 4080 1339 276 168 105 87 21 21 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 2921 2942 2963 2984 3005 3068 1108 Size: 1813 21 21 21 21 63 4137 Fragments arranged by size: 4137 1813 63 21 21 21 21 PfoI T'CCnGG_A Cuts at: 3004 3280 3004 Size: 276 5821 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2570 2602 4166 4198 5679 5711 2570 Size: 32 1564 32 1481 32 2956 Fragments arranged by size: 2956 1564 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2131 2131 Size: 6097 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2822 2822 Size: 6097 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2101 2101 Size: 6097 RsrII CG'GwC_CG Cuts at: 2491 2491 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 3445 4580 5261 5452 826 Size: 219 2400 1135 681 191 1471 Fragments arranged by size: 2400 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2279 2279 Size: 6097 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 2026 2349 2829 3028 3135 3219 3241 3424 Size: 154 323 480 199 107 84 22 183 Cuts at: 3424 1872 Size: 4545 Fragments arranged by size: 4545 480 323 199 183 154 107 84 22 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SrfI GCCC'GGGC Cuts at: 2349 2349 Size: 6097 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 2778 1517 Size: 1261 4836 StyI C'CwwG_G Cuts at: 513 1065 2004 2265 2274 3536 513 Size: 552 939 261 9 1262 3074 Fragments arranged by size: 3074 1262 939 552 261 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2680 2806 2988 3051 3072 3093 3159 Size: 1255 1100 126 182 63 21 21 66 Cuts at: 3159 3201 4124 4141 4294 4479 4924 325 Size: 42 923 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1100 923 445 185 182 153 126 66 63 42 21 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2144 2176 2655 2687 6057 6089 2144 Size: 32 479 32 3370 32 2152 Fragments arranged by size: 3370 2152 479 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 2024 2347 2827 3026 3133 3217 3239 3422 Size: 154 323 480 199 107 84 22 183 Cuts at: 3422 1870 Size: 4545 Fragments arranged by size: 4545 480 323 199 183 154 107 84 22 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SapI SexAI SfoI SwaI XcmI