(Linear) (Six-base) MAPSORT of: KIEE1519.seq Check: 8496 from: 1 to: 1392 KIEE1519.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 905 1392 Size: 905 487 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 565 597 1392 Size: 565 32 795 Fragments arranged by size: 795 565 32 AlwNI CAG_nnn'CTG Cuts at: 0 504 805 916 937 958 1000 1063 1392 Size: 504 301 111 21 21 42 63 329 Fragments arranged by size: 504 329 301 111 63 42 21 21 ApaI G_GGCC'C Cuts at: 0 821 1392 Size: 821 571 ApaLI G'TGCA_C Cuts at: 0 1135 1177 1303 1392 Size: 1135 42 126 89 Fragments arranged by size: 1135 126 89 42 AvaI C'yCGr_G Cuts at: 0 19 342 822 1021 1128 1212 1234 1392 Size: 19 323 480 199 107 84 22 158 Fragments arranged by size: 480 323 199 158 107 84 22 19 BanI G'GyrC_C Cuts at: 0 546 1392 Size: 546 846 BanII G_rGCy'C Cuts at: 0 157 524 821 1392 Size: 157 367 297 571 Fragments arranged by size: 571 367 297 157 BbvCI CC'TCA_GC Cuts at: 0 1268 1333 1392 Size: 1268 65 59 Fragments arranged by size: 1268 65 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 517 543 551 577 1392 Size: 517 26 8 26 815 Fragments arranged by size: 815 517 26 26 8 BglI GCCn_nnn'nGGC Cuts at: 0 23 265 274 344 729 816 1392 Size: 23 242 9 70 385 87 576 Fragments arranged by size: 576 385 242 87 70 23 9 Bme1580I G_kGCm'C Cuts at: 0 821 1139 1181 1307 1392 Size: 821 318 42 126 85 Fragments arranged by size: 821 318 126 85 42 BmgBI CAC'GTC Cuts at: 0 442 1392 Size: 442 950 BmrI ACTGGGnnnn_n' Cuts at: 0 868 1392 Size: 868 524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 607 836 998 1022 1392 Size: 124 483 229 162 24 370 Fragments arranged by size: 483 370 229 162 124 24 Bpu10I CC'TnA_GC Cuts at: 0 1268 1333 1392 Size: 1268 65 59 Fragments arranged by size: 1268 65 59 BsaI GGTCTCn'nnnn_ Cuts at: 0 100 523 598 1392 Size: 100 423 75 794 Fragments arranged by size: 794 423 100 75 BsaHI Gr'CG_yC Cuts at: 0 920 941 1392 Size: 920 21 451 Fragments arranged by size: 920 451 21 BsaWI w'CCGG_w Cuts at: 0 483 1392 Size: 483 909 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 139 169 565 595 650 680 1392 Size: 139 30 396 30 55 30 712 Fragments arranged by size: 712 396 139 55 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 42 45 385 1392 Size: 42 3 340 1007 Fragments arranged by size: 1007 340 42 3 BseYI C'CCAG_C Cuts at: 0 284 661 833 909 930 951 993 1140 Size: 284 377 172 76 21 21 42 147 Cuts at: 1140 1392 Size: 252 Fragments arranged by size: 377 284 252 172 147 76 42 21 21 BsiEI CG_ry'CG Cuts at: 0 339 1392 Size: 339 1053 BsiHKAI G_wGCw'C Cuts at: 0 1139 1181 1307 1392 Size: 1139 42 126 85 Fragments arranged by size: 1139 126 85 42 BsiWI C'GTAC_G Cuts at: 0 461 1392 Size: 461 931 BsmI GAATG_Cn' Cuts at: 0 169 1392 Size: 169 1223 Bsp1286I G_dGCh'C Cuts at: 0 157 524 821 1139 1181 1307 1392 Size: 157 367 297 318 42 126 85 Fragments arranged by size: 367 318 297 157 126 85 42 BspEI T'CCGG_A Cuts at: 0 483 1392 Size: 483 909 BspHI T'CATG_A Cuts at: 0 137 1392 Size: 137 1255 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 512 1392 Size: 512 880 BsrBI CCG'CTC Cuts at: 0 38 1392 Size: 38 1354 BsrDI GCAATG_nn' Cuts at: 0 533 1392 Size: 533 859 BsrFI r'CCGG_y Cuts at: 0 488 1392 Size: 488 904 BstAPI GCAn_nnn'nTGC Cuts at: 0 173 504 1392 Size: 173 331 888 Fragments arranged by size: 888 331 173 Bsu36I CC'TnA_GG Cuts at: 0 813 1163 1205 1392 Size: 813 350 42 187 Fragments arranged by size: 813 350 187 42 EaeI y'GGCC_r Cuts at: 0 336 512 730 1392 Size: 336 176 218 662 Fragments arranged by size: 662 336 218 176 EagI C'GGCC_G Cuts at: 0 336 1392 Size: 336 1056 EarI CTCTTCn'nnn_ Cuts at: 0 403 1392 Size: 403 989 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 586 1392 Size: 586 806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 607 836 998 1022 1392 Size: 124 483 229 162 24 370 Fragments arranged by size: 483 370 229 162 124 24 EcoO109I rG'GnC_Cy Cuts at: 0 126 276 802 817 818 1125 1167 1209 Size: 126 150 526 15 1 307 42 42 Cuts at: 1209 1337 1392 Size: 128 55 Fragments arranged by size: 526 307 150 128 126 55 42 42 15 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 633 1392 Size: 633 759 HaeII r_GCGC'y Cuts at: 0 87 495 1392 Size: 87 408 897 Fragments arranged by size: 897 408 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 465 497 702 734 1188 1220 1392 Size: 465 32 205 32 454 32 172 Fragments arranged by size: 465 454 205 172 32 32 32 MslI CAynn'nnrTG Cuts at: 0 191 1392 Size: 191 1201 MspA1I CmG'CkG Cuts at: 0 96 708 738 1108 1392 Size: 96 612 30 370 284 Fragments arranged by size: 612 370 284 96 30 PasI CC'CwG_GG Cuts at: 0 682 958 1063 1084 1171 1192 1360 1392 Size: 682 276 105 21 87 21 168 32 Fragments arranged by size: 682 276 168 105 87 32 21 21 PflMI CCAn_nnn'nTGG Cuts at: 0 916 937 958 979 1000 1063 1392 Size: 916 21 21 21 21 63 329 Fragments arranged by size: 916 329 63 21 21 21 21 PfoI T'CCnGG_A Cuts at: 0 999 1275 1392 Size: 999 276 117 Fragments arranged by size: 999 276 117 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 565 597 1392 Size: 565 32 795 Fragments arranged by size: 795 565 32 PpuMI rG'GwC_Cy Cuts at: 0 126 1392 Size: 126 1266 PspOMI G'GGCC_C Cuts at: 0 817 1392 Size: 817 575 PvuII CAG'CTG Cuts at: 0 96 1392 Size: 96 1296 RsrII CG'GwC_CG Cuts at: 0 486 1392 Size: 486 906 SfiI GGCCn_nnn'nGGCC Cuts at: 0 274 1392 Size: 274 1118 SmaI CCC'GGG Cuts at: 0 21 344 824 1023 1130 1214 1236 1392 Size: 21 323 480 199 107 84 22 156 Fragments arranged by size: 480 323 199 156 107 84 22 21 SrfI GCCC'GGGC Cuts at: 0 344 1392 Size: 344 1048 StuI AGG'CCT Cuts at: 0 773 1392 Size: 773 619 StyI C'CwwG_G Cuts at: 0 260 269 1392 Size: 260 9 1123 Fragments arranged by size: 1123 260 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 675 801 983 1046 1067 1088 1154 1196 Size: 675 126 182 63 21 21 66 42 Cuts at: 1196 1392 Size: 196 Fragments arranged by size: 675 196 182 126 66 63 42 21 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 139 171 650 682 1392 Size: 139 32 479 32 710 Fragments arranged by size: 710 479 139 32 32 XmaI C'CCGG_G Cuts at: 0 19 342 822 1021 1128 1212 1234 1392 Size: 19 323 480 199 107 84 22 158 Fragments arranged by size: 480 323 199 158 107 84 22 19 Enzymes that do cut: AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbvCI BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI Bsu36I EaeI EagI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I MslI MspA1I PasI PflMI PfoI PpiI PpuMI PspOMI PvuII RsrII SfiI SmaI SrfI StuI StyI TaqII TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI BsaAI BsaBI BsgI BsmBI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI