################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MboI MflI MluI MluNI MroI MscI MseI MslI MspA1I MvnI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 420 ACACGT A^C[GA][CT]GT AlwNI 292 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AvrII 450 CCTAGG C^CTAGG BcnI 503 CCCGG CC^[GC]GG BglI 437 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 450 CCTAGG C^CTAGG BsmI 315 GAATGC GAATGC(1/-1) BstF5I 561 CATCC GGATG(2/0) DraII 21 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 389 CTCTTC CTCTTC(1/4) EcoNI 450 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 21 AGGTCCT [GA]G^G[ACGT]CC[CT] FauI 464 CCCGC CCCGC(4/6) FokI 561 CATCC GGATG(9/13) HapII 504 CCGG C^CGG HgaI 260 GACGC GACGC(5/10) HincII 475 GTCGAC GT[CT]^[GA]AC HindII 475 GTCGAC GT[CT]^[GA]AC HpaII 504 CCGG C^CGG Ksp632I 389 CTCTTC CTCTTC(1/4) MfeI 369 CAATTG C^AATTG MspI 504 CCGG C^CGG MunI 369 CAATTG C^AATTG NciI 503 CCCGG CC^[GC]GG NcoI 209 CCATGG C^CATGG PflMI 603 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 268 GACTC GAGTC(4/5) PpuMI 21 AGGTCCT [GA]G^G[AT]CC[CT] PstI 298 CTGCAG CTGCA^G SalI 475 GTCGAC G^TCGAC SapI 388 GCTCTTC GCTCTTC(1/4) ScaI 326 AGTACT AGT^ACT SfcI 298 CTGCAG C^T[GA][CT]AG TaqI 476 TCGA T^CGA TfiI 620 GATTC G^A[AT]TC Tsp509I 370 AATT ^AATT TspRI 493 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 476 TCGA T^CGA Van91I 603 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 209 CCATGGGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 424 GTCTAC GT^[AC][GT]AC 475 GTCGAC AfaI 327 GTAC GT^AC 613 GTAC AluI 109 AGCT AG^CT 230 AGCT AvaII 22 GGTCC G^G[AT]CC 556 GGTCC BfaI 200 CTAG C^TAG 451 CTAG BseRI 70 GAGGAG GAGGAG(10/8) 94 GAGGAG BsrDI 498 CATTGC GCAATG(2/0) 551 GCAATG BsrI 442 ACTGG ACTGG(1/-1) 603 CCAGT BstAPI 291 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 310 GCAGAGAATGC BstEII 180 GGTCACC G^GT[ACGT]ACC 459 GGTGACC BstPI 180 GGTCACC G^GT[ACGT]ACC 459 GGTGACC Cac8I 48 GCCTGC GC[ACGT]^[ACGT]GC 231 GCTCGC DdeI 68 CTGAG C^T[ACGT]AG 270 CTCAG DsaI 209 CCATGG C^C[GA][CT]GG 305 CCACGG EcoO65I 180 GGTCACC G^GT[ACGT]ACC 459 GGTGACC EcoT14I 209 CCATGG C^C[AT][AT]GG 450 CCTAGG HinfI 268 GACTC G^A[ACGT]TC 620 GATTC MaeI 200 CTAG C^TAG 451 CTAG MaeIII 181 GTCAC ^GT[ACGT]AC 460 GTGAC NlaIV 374 GGGGCC GG[ACGT]^[ACGT]CC 556 GGTCCC RsaI 327 GTAC GT^AC 613 GTAC SfaNI 365 GCATC GCATC(5/9) 489 GCATC StyI 209 CCATGG C^C[AT][AT]GG 450 CCTAGG Tsp45I 181 GTCAC ^GT[GC]AC 460 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BsmFI BspMI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII HphI ItaI MaeII MboII MnlI MvaI MwoI NlaIII Sau96I ScrFI TseI