################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI Ksp632I KspI MaeII MamI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 638 GGTACC G^GTACC AfaI 639 GTAC GT^AC AflII 37 CTTAAG C^TTAAG Asp700I 105 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 638 GGTACC G^GTACC BbvCI 919 GCTGAGG CCTCAGC(-5/-2) BfaI 511 CTAG C^TAG BfrI 37 CTTAAG C^TTAAG BsgI 827 GTGCAG GTGCAG(16/14) BspHI 191 TCATGA T^CATGA BssSI 530 CACGAG CACGAG(-5/-1) BstAPI 469 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 815 CCATGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 977 AGATCC [GA]^GATC[CT] DraII 786 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO109I 786 AGGCCCC [GA]G^G[ACGT]CC[CT] HincII 628 GTTGAC GT[CT]^[GA]AC HindII 628 GTTGAC GT[CT]^[GA]AC HindIII 172 AAGCTT A^AGCTT KpnI 638 GGTACC GGTAC^C MaeI 511 CTAG C^TAG MboII 930 GAAGA GAAGA(8/7) MfeI 633 CAATTG C^AATTG MflI 977 AGATCC [GA]^GATC[CT] MseI 38 TTAA T^TAA MunI 633 CAATTG C^AATTG NdeI 875 CATATG CA^TATG NspI 729 GCATGT [GA]CATG^[CT] RcaI 191 TCATGA T^CATGA RsaI 639 GTAC GT^AC SacI 151 GAGCTC GAGCT^C SpeI 510 ACTAGT A^CTAGT Tru9I 38 TTAA T^TAA XcmI 341 CCATTGCCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 977 AGATCC [GA]^GATC[CT] XmnI 105 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 978 GATCC GGATC(4/5) 279 GGATC AspHI 151 GAGCTC G[AT]GC[AT]^C 439 GTGCTC BpmI 836 CTGGAG CTGGAG(16/14) 949 CTGGAG BsiHKAI 151 GAGCTC G[AT]GC[AT]^C 439 GTGCTC BspMI 493 ACCTGC ACCTGC(4/8) 321 GCAGGT BsrDI 342 CATTGC GCAATG(2/0) 799 GCAATG BsrI 451 ACTGG ACTGG(1/-1) 835 ACTGG EcoNI 303 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 700 CCTCAGTTAGG MaeIII 182 GTCAC ^GT[ACGT]AC 646 GTCAC PleI 880 GAGTC GAGTC(4/5) 913 GAGTC PstI 495 CTGCAG CTGCA^G 991 CTGCAG SfcI 495 CTGCAG C^T[GA][CT]AG 991 CTGCAG Tsp45I 182 GTCAC ^GT[GC]AC 646 GTCAC Tsp509I 521 AATT ^AATT 634 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AlwNI AvaII BanI BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MnlI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SmlI TfiI TseI TspRI