################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BcnI BfaI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluNI MroI MscI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 598 AAATTC [GA]^AATT[CT] AcyI 310 GACGCC G[GA]^CG[CT]C AflIII 811 ACGCGT A^C[GA][CT]GT AhdI 412 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 615 GTGCAC G^TGCAC ApaLI 615 GTGCAC G^TGCAC ApoI 598 AAATTC [GA]^AATT[CT] AspEI 412 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 470 GGATCC G^GATCC BanII 232 GAGCTC G[GA]GC[CT]^C BbvCI 906 CCTCAGC CCTCAGC(-5/-2) BclI 917 TGATCA T^GATCA BglI 962 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 957 GCTGAGC GC^T[ACGT]AGC Bpu1102I 957 GCTGAGC GC^T[ACGT]AGC BsaHI 310 GACGCC G[GA]^CG[CT]C BspDI 133 ATCGAT AT^CGAT BspMI 635 ACCTGC ACCTGC(4/8) CelII 957 GCTGAGC GC^T[ACGT]AGC CfoI 621 GCGC GCG^C ClaI 133 ATCGAT AT^CGAT DrdI 219 GACCTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 708 CCATGG C^C[GA][CT]GG Eam1105I 412 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 917 TGATCA T^GATCA HhaI 621 GCGC GCG^C Hin1I 310 GACGCC G[GA]^CG[CT]C HinP1I 621 GCGC G^CGC MluI 811 ACGCGT A^CGCGT MseI 862 TTAA T^TAA NcoI 708 CCATGG C^CATGG PleI 782 GACTC GAGTC(4/5) PstI 487 CTGCAG CTGCA^G PvuII 379 CAGCTG CAG^CTG SacI 232 GAGCTC GAGCT^C SapI 45 GAAGAGC GCTCTTC(1/4) SbfI 486 CCTGCAGG CCTGCA^GG SfcI 487 CTGCAG C^T[GA][CT]AG Sse8387I 486 CCTGCAGG CCTGCA^GG TaqI 134 TCGA T^CGA TfiI 576 GATTC G^A[AT]TC Tru9I 862 TTAA T^TAA TthHB8I 134 TCGA T^CGA XcmI 728 CCATGCTCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 620 CGCG CG^CG 812 CGCG AfaI 273 GTAC GT^AC 894 GTAC AlwNI 68 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 550 CAGGATCTG BbsI 409 GAAGAC GAAGAC(2/6) 925 GAAGAC BpuAI 409 GAAGAC GAAGAC(2/6) 925 GAAGAC BseRI 430 GAGGAG GAGGAG(10/8) 721 GAGGAG BsrI 531 ACTGG ACTGG(1/-1) 717 ACTGG BstUI 620 CGCG CG^CG 812 CGCG DraII 206 AGGACCT [GA]G^G[ACGT]CC[CT] 820 GGGACCC EarI 45 GAAGAG CTCTTC(1/4) 652 GAAGAG EcoNI 426 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 483 CCTCCTGCAGG EcoO109I 206 AGGACCT [GA]G^G[ACGT]CC[CT] 820 GGGACCC FauI 276 CCCGC CCCGC(4/6) 384 GCGGG HinfI 576 GATTC G^A[ACGT]TC 782 GACTC Ksp632I 45 GAAGAG CTCTTC(1/4) 652 GAAGAG MvnI 620 CGCG CG^CG 812 CGCG MwoI 162 GCAGCGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 962 GCCTGGTGGGC PpuMI 206 AGGACCT [GA]G^G[AT]CC[CT] 820 GGGACCC RsaI 273 GTAC GT^AC 894 GTAC SfaNI 76 GATGC GCATC(5/9) 692 GCATC Tsp509I 599 AATT ^AATT 666 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BbvI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspA1I MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI Tsp45I TspRI XhoII