(Circular) (Six-base) MAPSORT of: pFN21AE1118.seq Check: 6587 from: 1 to: 5686 pFN21AE1118 5686 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2649 2649 Size: 5686 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3749 5133 5401 1632 Size: 2117 1384 268 1917 Fragments arranged by size: 2117 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 2816 4585 5562 1203 Size: 1613 1769 977 1327 Fragments arranged by size: 1769 1613 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 2422 4415 2422 Size: 1993 3693 AleI CACnn'nnGTG Cuts at: 1166 2744 1166 Size: 1578 4108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 2078 2560 3429 5001 2078 Size: 482 869 1572 2763 Fragments arranged by size: 2763 1572 869 482 ApaLI G'TGCA_C Cuts at: 2620 3743 4899 2620 Size: 1123 1156 3407 Fragments arranged by size: 3407 1156 1123 ApoI r'AATT_y Cuts at: 1493 2603 2995 3221 3373 5482 1493 Size: 1110 392 226 152 2109 1697 Fragments arranged by size: 2109 1697 1110 392 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BamHI G'GATC_C Cuts at: 2475 3016 3383 2475 Size: 541 367 4778 Fragments arranged by size: 4778 541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3007 4462 618 Size: 325 217 627 1220 1455 1842 Fragments arranged by size: 1842 1455 1220 627 325 217 BanII G_rGCy'C Cuts at: 1391 2241 3005 1391 Size: 850 764 4072 Fragments arranged by size: 4072 850 764 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2421 2937 928 Size: 971 522 516 3677 Fragments arranged by size: 3677 971 522 516 BbvCI CC'TCA_GC Cuts at: 2912 2912 Size: 5686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 2922 1548 Size: 1374 4312 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2973 4297 136 Size: 107 122 71 631 605 1301 1324 1525 Fragments arranged by size: 1525 1324 1301 631 605 122 107 71 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 2963 3103 2963 Size: 140 5546 Bme1580I G_kGCm'C Cuts at: 1790 2624 3747 4903 1790 Size: 834 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 834 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3417 4375 442 Size: 416 53 83 399 24 2000 958 1753 Fragments arranged by size: 2000 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2489 2744 4346 1239 Size: 1214 36 255 1602 2579 Fragments arranged by size: 2579 1602 1214 255 36 Bpu10I CC'TnA_GC Cuts at: 2329 2912 2329 Size: 583 5103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 2216 2702 4349 882 Size: 617 582 135 486 1647 2219 Fragments arranged by size: 2219 1647 617 582 486 135 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 3493 3875 4510 Size: 53 83 186 936 783 1177 382 635 Cuts at: 4510 275 Size: 1451 Fragments arranged by size: 1451 1177 936 783 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2526 2556 1178 Size: 30 165 30 1123 30 4308 Fragments arranged by size: 4308 1123 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2450 2741 1186 Size: 1264 291 4131 Fragments arranged by size: 4131 1264 291 BseYI C'CCAG_C Cuts at: 4889 5385 4889 Size: 496 5190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2241 2624 3747 3832 4903 2148 Size: 93 383 1123 85 1071 2931 Fragments arranged by size: 2931 1123 1071 383 93 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2241 2624 3005 3747 3832 4903 Size: 399 358 93 383 381 742 85 1071 Cuts at: 4903 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 383 381 358 93 85 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2649 3041 844 Size: 1805 392 3489 Fragments arranged by size: 3489 1805 392 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4181 4355 65 Size: 1183 2933 174 1396 Fragments arranged by size: 2933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4330 4517 1700 Size: 110 2520 187 2869 Fragments arranged by size: 2869 2520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2358 2475 2557 3016 3383 3768 3785 5237 Size: 386 117 82 459 367 385 17 1452 Cuts at: 5237 5323 5335 5681 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 459 386 385 367 346 117 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2713 513 Size: 552 939 709 3486 Fragments arranged by size: 3486 939 709 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 2635 3202 3996 4016 2635 Size: 567 794 20 4305 Fragments arranged by size: 4305 794 567 20 ClaI AT'CG_AT Cuts at: 1554 2139 1554 Size: 585 5101 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2230 4693 809 Size: 507 914 2463 1802 Fragments arranged by size: 2463 1802 914 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3133 4022 5651 Size: 54 1425 50 275 18 1303 889 1629 Cuts at: 5651 8 Size: 43 Fragments arranged by size: 1629 1425 1303 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 2652 3616 1353 Size: 692 607 964 3423 Fragments arranged by size: 3423 964 692 607 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4273 4647 4793 387 Size: 1064 138 2684 374 146 1280 Fragments arranged by size: 2684 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2239 2239 Size: 5686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2489 2744 3749 4346 5133 5401 Size: 393 821 36 255 1005 597 787 268 Cuts at: 5401 1239 Size: 1524 Fragments arranged by size: 1524 1005 821 787 597 393 268 255 36 EcoNI CCTnn'n_nnAGG Cuts at: 2435 2492 2435 Size: 57 5629 EcoO109I rG'GnC_Cy Cuts at: 2212 2826 2212 Size: 614 5072 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 4833 1678 Size: 3155 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2408 2440 2526 2558 4327 Size: 32 135 32 836 32 86 32 1769 Cuts at: 4327 4359 4401 4433 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1769 836 135 86 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 2816 5562 2816 Size: 2746 2940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3864 Fragments arranged by size: 3864 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2744 2924 3644 4003 4162 518 Size: 648 975 603 180 720 359 159 2042 Fragments arranged by size: 2042 975 720 648 603 359 180 159 MspA1I CmG'CkG Cuts at: 1606 2170 2386 2940 3102 3779 4927 5172 1606 Size: 564 216 554 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 564 554 245 216 162 NcoI C'CATG_G Cuts at: 513 1065 2004 2713 513 Size: 552 939 709 3486 Fragments arranged by size: 3486 939 709 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 3044 4589 1132 Size: 97 1815 1545 2229 Fragments arranged by size: 2229 1815 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4784 PfoI T'CCnGG_A Cuts at: 2021 2021 Size: 5686 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2826 2212 Size: 614 5072 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 2496 3038 830 Size: 1666 542 3478 Fragments arranged by size: 3478 1666 542 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 PvuII CAG'CTG Cuts at: 2386 2386 Size: 5686 SacI G_AGCT'C Cuts at: 2241 2241 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SanDI GG'GwC_CC Cuts at: 2826 2826 Size: 5686 SapI GCTCTTCn'nnn_ Cuts at: 2045 2045 Size: 5686 SbfI CC_TGCA'GG Cuts at: 2496 3038 2496 Size: 542 5144 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2492 3034 4169 4850 5041 826 Size: 219 1447 542 1135 681 191 1471 Fragments arranged by size: 1471 1447 1135 681 542 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5686 StyI C'CwwG_G Cuts at: 513 1065 2004 2301 2337 2713 3125 513 Size: 552 939 297 36 376 412 3074 Fragments arranged by size: 3074 939 552 412 376 297 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2771 3713 3730 3883 4068 4513 325 Size: 1255 1191 942 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1191 942 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5646 5678 5646 Size: 32 5654 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2740 2740 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI