################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmBI BsmI BspDI BsrDI BsrFI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 74 TCCGGA T^CCGGA AcsI 706 AAATTC [GA]^AATT[CT] AflIII 1094 ACATGT A^C[GA][CT]GT AhdI 973 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 706 AAATTC [GA]^AATT[CT] AspEI 973 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 289 GAGCTC G[AT]GC[AT]^C AviII 1034 TGCGCA TGC^GCA BalI 720 TGGCCA TGG^CCA BbeI 379 GGCGCC GGCGC^C BbsI 264 GAAGAC GAAGAC(2/6) BfaI 202 CTAG C^TAG BglII 1001 AGATCT A^GATCT BpuAI 264 GAAGAC GAAGAC(2/6) BsaAI 652 TACGTG [CT]AC^GT[GA] BsaI 514 GAGACC GGTCTC(1/5) BsaWI 74 TCCGGA [AT]^CCGG[AT] BseAI 74 TCCGGA T^CCGGA BsgI 154 CTGCAC GTGCAG(16/14) BsiHKAI 289 GAGCTC G[AT]GC[AT]^C Bsp1407I 554 TGTACA T^GTACA BspEI 74 TCCGGA T^CCGGA BspLU11I 1094 ACATGT A^CATGT BsrBI 1275 CCGCTC CCGCTC(-3/-3) BsrGI 554 TGTACA T^GTACA BstAPI 1191 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 55 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 973 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 55 AGGACCC [GA]G^G[ACGT]CC[CT] FauI 32 CCCGC CCCGC(4/6) FspI 1034 TGCGCA TGC^GCA KasI 379 GGCGCC G^GCGCC KspI 245 CCGCGG CCGC^GG MaeI 202 CTAG C^TAG MluNI 720 TGGCCA TGG^CCA MroI 74 TCCGGA T^CCGGA MscI 720 TGGCCA TGG^CCA MseI 1121 TTAA T^TAA NarI 379 GGCGCC GG^CGCC NspI 1094 ACATGT [GA]CATG^[CT] PpuMI 55 AGGACCC [GA]G^G[AT]CC[CT] PshAI 612 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 85 CAGCTG CAG^CTG SacI 289 GAGCTC GAGCT^C SacII 245 CCGCGG CCGC^GG SapI 1248 GAAGAGC GCTCTTC(1/4) SfoI 379 GGCGCC GGC^GCC SmaI 836 CCCGGG CCC^GGG SspBI 554 TGTACA T^GTACA TfiI 851 GAATC G^A[AT]TC Tru9I 1121 TTAA T^TAA XmaCI 836 CCCGGG C^CCGGG XmaI 836 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 267 GACGCC G[GA]^CG[CT]C 379 GGCGCC Asp700I 804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1248 GAAGAGCTTC BamHI 77 GGATCC G^GATCC 1231 GGATCC BsaHI 267 GACGCC G[GA]^CG[CT]C 379 GGCGCC BspHI 587 TCATGA T^CATGA 710 TCATGA BspMI 152 ACCTGC ACCTGC(4/8) 727 ACCTGC BssHII 27 GCGCGC G^CGCGC 137 GCGCGC EarI 639 GAAGAG CTCTTC(1/4) 1248 GAAGAG EcoT14I 371 CCATGG C^C[AT][AT]GG 1239 CCATGG HgaI 267 GACGC GACGC(5/10) 689 GACGC Hin1I 267 GACGCC G[GA]^CG[CT]C 379 GGCGCC Ksp632I 639 GAAGAG CTCTTC(1/4) 1248 GAAGAG NcoI 371 CCATGG C^CATGG 1239 CCATGG PleI 276 GACTC GAGTC(4/5) 642 GAGTC RcaI 587 TCATGA T^CATGA 710 TCATGA StuI 1291 AGGCCT AGG^CCT 1311 AGGCCT StyI 371 CCATGG C^C[AT][AT]GG 1239 CCATGG Tsp509I 707 AATT ^AATT 777 AATT XcmI 362 CCATCCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1145 CCAGTGGTGGGGTGG XmnI 804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1248 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoNI EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII