(Circular) (Six-base) MAPSORT of: pFN21AE1360.seq Check: 8653 from: 1 to: 5422 pFN21AE1360 5422 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2047 3485 4869 5137 1632 Size: 415 1438 1384 268 1917 Fragments arranged by size: 1917 1438 1384 415 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 4321 5298 1203 Size: 3118 977 1327 Fragments arranged by size: 3118 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AlwNI CAG_nnn'CTG Cuts at: 3165 4737 3165 Size: 1572 3850 ApaI G_GGCC'C Cuts at: 2191 2191 Size: 5422 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2093 2731 2957 3109 5218 1493 Size: 600 638 226 152 2109 1697 Fragments arranged by size: 2109 1697 638 600 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2747 1870 Size: 73 804 4545 Fragments arranged by size: 4545 804 73 BamHI G'GATC_C Cuts at: 2376 2752 3119 2376 Size: 376 367 4679 Fragments arranged by size: 4679 376 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2743 4198 618 Size: 325 217 627 956 1455 1842 Fragments arranged by size: 1842 1455 956 627 325 217 BanII G_rGCy'C Cuts at: 1391 2191 2610 2741 1391 Size: 800 419 131 4072 Fragments arranged by size: 4072 800 419 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2053 928 Size: 971 154 4297 Fragments arranged by size: 4297 971 154 BbvCI CC'TCA_GC Cuts at: 2088 2088 Size: 5422 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BfrBI ATG'CAT Cuts at: 2358 2358 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4033 136 Size: 107 122 71 631 605 2361 1525 Fragments arranged by size: 2361 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2646 2839 2646 Size: 193 5229 Bme1580I G_kGCm'C Cuts at: 1790 2191 3483 4639 1790 Size: 401 1292 1156 2573 Fragments arranged by size: 2573 1292 1156 401 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3153 4111 442 Size: 416 53 83 399 24 1736 958 1753 Fragments arranged by size: 1753 1736 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2696 4082 1239 Size: 1226 231 1386 2579 Fragments arranged by size: 2579 1386 1226 231 Bpu10I CC'TnA_GC Cuts at: 2030 2088 2609 2030 Size: 58 521 4843 Fragments arranged by size: 4843 521 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3547 4412 4710 3547 Size: 865 298 4259 Fragments arranged by size: 4259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4085 882 Size: 617 2586 2219 Fragments arranged by size: 2586 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3213 493 Size: 711 2009 2702 Fragments arranged by size: 2702 2009 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3229 3611 4246 275 Size: 53 83 186 936 1696 382 635 1451 Fragments arranged by size: 1696 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5197 Fragments arranged by size: 5197 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5422 BseYI C'CCAG_C Cuts at: 4625 5121 4625 Size: 496 4926 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4849 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2872 3633 4661 1540 Size: 275 186 871 761 1028 2301 Fragments arranged by size: 2301 1028 871 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2610 3483 3568 4639 2610 Size: 873 85 1071 3393 Fragments arranged by size: 3393 1071 873 85 BsmI GAATG_Cn' Cuts at: 2358 2461 2933 3026 2358 Size: 103 472 93 4754 Fragments arranged by size: 4754 472 103 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2191 2610 2741 3483 3568 4639 1391 Size: 399 401 419 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 419 401 399 131 85 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 844 Size: 1933 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2034 3309 1394 Size: 429 211 1275 3507 Fragments arranged by size: 3507 1275 429 211 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4066 4253 1700 Size: 110 2256 187 2869 Fragments arranged by size: 2869 2256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 3482 4494 3482 Size: 1012 4410 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5422 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstXI CCAn_nnnn'nTGG Cuts at: 2337 2623 2337 Size: 286 5136 BstYI r'GATC_y Cuts at: 1972 2376 2752 3119 3504 3521 4973 5059 5071 Size: 404 376 367 385 17 1452 86 12 Cuts at: 5071 5417 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 404 385 376 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2630 2630 Size: 5422 BtgI C'CryG_G Cuts at: 513 1065 2004 2616 513 Size: 552 939 612 3319 Fragments arranged by size: 3319 939 612 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4683 Fragments arranged by size: 4683 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3732 3752 2938 Size: 794 20 4608 Fragments arranged by size: 4608 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 1999 2034 529 Size: 35 1435 35 3917 Fragments arranged by size: 3917 1435 35 35 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2529 2869 Size: 54 1425 50 275 18 177 522 340 Cuts at: 2869 3758 5387 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 522 340 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 1537 Size: 275 1057 4090 Fragments arranged by size: 4090 1057 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3352 1353 Size: 1999 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4009 4383 4529 387 Size: 1064 138 2420 374 146 1280 Fragments arranged by size: 2420 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2608 2608 Size: 5422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2465 2696 3485 4082 4869 5137 Size: 393 415 418 231 789 597 787 268 Cuts at: 5137 1239 Size: 1524 Fragments arranged by size: 1524 789 787 597 418 415 393 268 231 EcoO109I rG'GnC_Cy Cuts at: 2188 2634 2188 Size: 446 4976 EcoRI G'AATT_C Cuts at: 2093 2731 3109 2093 Size: 638 378 4406 Fragments arranged by size: 4406 638 378 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 4569 1678 Size: 2891 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373 Size: 32 135 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2689 2766 3012 669 Size: 988 281 751 77 246 3079 Fragments arranged by size: 3079 988 751 281 246 77 HindIII A'AGCT_T Cuts at: 748 2478 748 Size: 1730 3692 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2404 4536 4720 1305 Size: 1099 2132 184 2007 Fragments arranged by size: 2132 2007 1099 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2531 10 Size: 54 1425 343 177 522 2901 Fragments arranged by size: 2901 1425 522 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 3380 3739 3898 518 Size: 648 1197 1017 359 159 2042 Fragments arranged by size: 2042 1197 1017 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2838 3515 4663 4908 1606 Size: 1232 677 1148 245 2120 Fragments arranged by size: 2120 1232 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2616 513 Size: 552 939 612 3319 Fragments arranged by size: 3319 939 612 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NsiI A_TGCA'T Cuts at: 2360 2360 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2780 4325 1132 Size: 97 1551 1545 2229 Fragments arranged by size: 2229 1551 1545 97 PasI CC'CwG_GG Cuts at: 1348 2288 1348 Size: 940 4482 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5422 PfoI T'CCnGG_A Cuts at: 2079 2430 2079 Size: 351 5071 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PspOMI G'GGCC_C Cuts at: 2187 2187 Size: 5422 PstI C_TGCA'G Cuts at: 830 2774 830 Size: 1944 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 SacI G_AGCT'C Cuts at: 2610 2610 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 2504 3670 5382 2504 Size: 1166 1712 2544 Fragments arranged by size: 2544 1712 1166 SfcI C'TryA_G Cuts at: 826 1045 2101 2770 3905 4586 4777 826 Size: 219 1056 669 1135 681 191 1471 Fragments arranged by size: 1471 1135 1056 681 669 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4427 4689 820 Size: 197 926 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 2537 1517 Size: 1020 4402 StyI C'CwwG_G Cuts at: 513 1065 2004 2223 2532 2541 2571 2616 2861 Size: 552 939 219 309 9 30 45 245 Cuts at: 2861 513 Size: 3074 Fragments arranged by size: 3074 939 552 309 245 219 45 30 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2236 3449 3466 3619 3804 4249 325 Size: 1255 656 1213 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1213 656 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2502 3668 5380 Size: 274 80 33 51 1443 525 1166 1712 Cuts at: 5380 96 Size: 138 Fragments arranged by size: 1712 1443 1166 525 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 5382 5414 2184 Size: 32 3166 32 2192 Fragments arranged by size: 3166 2192 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5422 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 2075 3551 2075 Size: 1476 3946 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI